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Invitrogen
CD336 (NKp44) Monoclonal Antibody (44.189), PE, eBioscience™
FIGURE: 1 / 1
CD336 (NKp44) Antibody (12-3369-42) in Flow
Normal human peripheral blood cells were stimulated with Human IL-2 Recombinant Protein (Product # 14-8029-81) for 15 days, then stained with Anti-Human CD56 (NCAM) FITC (Product # 11-0566-42) and Mouse IgG2b K Isotype Control PE (Product # 12-4732-81) (left) or Anti-Human CD336 (NKp44) APC (right). Viable cells in the lymphocyte gate, as determined by Fixable Viability Dye eFluor® 450 (Product # 65-0863-14), were used for analysis.
Product Details
12-3369-42
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
44.189
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2572607
Product Specific Information
Description: The monoclonal antibody 44.189 reacts with human CD336, which is also known as NKp44 or NCR2. CD336 is a 44-kDa transmembrane glycoprotein belonging to the natural cytotoxicity receptor (NCR) family and contains an ITIM domain located in the cytoplasmic tail. Expression of CD336 and NK cell activation by the receptor requires association with DAP12, an ITAM-containing transmembrane accessory protein. Once thought to be expressed exclusively on IL-2-activated NK cells and a subset of gamma delta T lymphocytes, studies have shown expression of CD336 on IL-3-stimulated plasmacytoid dendritic cells, alpha beta T cells in the small intestinal epithelium, and T cells in cord blood. CD336 has been shown to be involved in the innate response of activated NK cells against viral infection and tumor cell proliferation.
The 44.189 antibody has been reported to facilitate CD336 crosslinking.
Applications Reported: This 44.189 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 44.189 antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD336 is also known as NKp44 or NCR2. CD336 is a 44-kDa transmembrane glycoprotein belonging to the natural cytotoxicity receptor (NCR) family and contains an ITIM domain located in the cytoplasmic tail. Expression of CD336 and NK cell activation by the receptor requires association with DAP12, an ITAM-containing transmembrane accessory protein. Once thought to be expressed exclusively on IL-2-activated NK cells and a subset of gamma delta T lymphocytes, studies have shown expression of CD336 on IL-3-stimulated plasmacytoid dendritic cells, alpha beta T cells in the small intestinal epithelium, and T cells in cord blood. CD336 has been shown to be involved in the innate response of activated NK cells against viral infection and tumor cell proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD336; CD336 antigen; lymphocyte antigen 95 (activating NK-receptor; NK-p44); Lymphocyte antigen 95 homolog; lymphocyte antigen 95 homolog (activating NK-receptor; NK-p44); Natural cytotoxicity triggering receptor 2; Natural killer cell p44-related protein; natural killer cell receptor; NK cell activating receptor (NKp44); NK cell-activating receptor; NK-p44; NK-p44dJ149M18.1
Gene Aliases: CD336; dJ149M18.1; LY95; NCR2; NK-p44; NKP44
UniProt ID: (Human) O95944
Entrez Gene ID: (Human) 9436
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