Invitrogen

CD326 (EpCAM) Monoclonal Antibody (G8.8), PE-Cyanine7, eBioscience™

CD326 (EpCAM) Antibody in Flow Cytometry (Flow)

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CD326 (EpCAM) Antibody (25-5791-80) in Flow

Staining of the TE-71 cell line with 0.06 µg of Rat IgG2a K Isotype Control PE-Cyanine7 (Product # 25-4321-82) (blue histogram) or 0.06 µg of Anti-Mouse CD326 (EpCAM) PE-Cyanine7 (purple histogram). Total viable cells were used for analysis.

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Product Details

25-5791-80

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Flow Cytometry (Flow)

0.125 µg/test

View 77 publications 77 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse, Pig

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

G8.8

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1724047

Product Specific Information

Description: The G8.8 monoclonal antibody reacts with the 40 kDa mouse EpCAM (epithelial cellular adhesion molecule), also known as EGP40 (epithelial glycoprotein 40), 17-1A antigen, TACSTD1 (tumor-associated calcium signal transducer 1), and CD326. The immunogen used to generate the G8.8 antibody was the TE-71 thymic epithelial cell line. CD326 is expressed on the majority of epithelial cells, and is considered a pan-carcinoma antigen. CD326 mediates calcium-independent, homophilic, cell-cell adhesion and may function as a growth factor receptor. The antigen is being used as a target for immunotherapy treatment of human carcinomas. CD326 binds LAIR-1 (CD305) and LAIR-2 (CD306) to inhibit cellular activation and inflammation. This epithelial glycoprotein is now recognized as having an important role in tumor biology.

Applications Reported: This G8.8 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This G8.8 antibody has been tested by flow cytometric analysis of the TE-71 cell line. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein expressed in the epithelium with a molecular weight of approximately 40 kDa, which functions as an epithelial cell adhesion molecule. Ep-CAM functions as a homotypic calcium-independent cell adhesion molecule, and has a direct impact on cell cycle, proliferation and metabolism of epithelial cells and fibroblasts due to its ability to rapidly induce the proto-oncogene c-myc and the cell cycle regulating genes cyclin A and E. Ep-CAM mediates Ca2+-independent homotypic interactions. Formation of Ep-CAM-mediated adhesions have a negative regulatory effect on adhesions mediated by classic cadherins, which may have strong effects on the differentiation and growth of epithelial cells. Ep-CAM overexpression was suggested to be associated with enhanced epithelial proliferation. Ep-CAM is highly expressed in human carcinomas, and is a marker for tumors of epithelial lineage. Ep-CAM is expressed on baso-lateral cell surface in most simple epithelia and many carcinoma types. Also, Ep-CAM reportedly distinguishes adenocarcinomas from pleural mesotheliomas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Dissociation and flow cytometric isolation of murine intestinal epithelial cells for multi-omic profiling.

STAR protocols

Ge Y,Zadeh M,Mohamadzadeh M

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-5791-80) in Flow Cytometry

Fri Mar 17 00:00:00 EDT 2023

Major depression-related factor NEGR1 controls salivary secretion in mouse submandibular glands.

iScience

Lee J,Kim S,Lee B,Kim YB,Kim KH,Chung G,Lee SJ,Lee S,Sun W,Park HK,Choi SY

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-5791-80) in Flow Cytometry

Fri May 19 00:00:00 EDT 2023

Endothelial AHR activity prevents lung barrier disruption in viral infection.

Nature

Major J,Crotta S,Finsterbusch K,Chakravarty P,Shah K,Frederico B,D'Antuono R,Green M,Meader L,Suarez-Bonnet A,Priestnall S,Stockinger B,Wack A

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-5791-80) in Flow Cytometry

Fri Sep 01 00:00:00 EDT 2023

Generation of liver mesenchyme and ductal cell organoid co-culture using cell self-aggregation and droplet microfluidics.

STAR protocols

Dowbaj AM,Kohler TN,Cordero-Espinoza L,Hollfelder F,Huch M

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-5791-80) in Flow Cytometry

Fri Jun 02 00:00:00 EDT 2023

Developmental diversity and unique sensitivity to injury of lung endothelial subtypes during postnatal growth.

iScience

Zanini F,Che X,Knutsen C,Liu M,Suresh NE,Domingo-Gonzalez R,Dou SH,Zhang D,Pryhuber GS,Jones RC,Quake SR,Cornfield DN,Alvira CM

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-5791-80) in Flow Cytometry

Fri Mar 17 00:00:00 EDT 2023

Inhibition of antiapoptotic BCL-2 proteins with ABT-263 induces fibroblast apoptosis, reversing persistent pulmonary fibrosis.

JCI insight

Cooley JC,Javkhlan N,Wilson JA,Foster DG,Edelman BL,Ortiz LA,Schwartz DA,Riches DW,Redente EF

25-5791-80 was used in Flow cytometry/Cell sorting to demonstrate that antiapoptotic BCL-2 family members are expressed by fibroblasts in both human and murine progressive fibrotic lung disease, that these fibroblasts are highly primed, and that treatment with ABT-263 induced fibroblast apoptosis and reduced fibrosis in 2 preclinical models of PF-ILD.

Wed Feb 08 00:00:00 EST 2023

Enteric viruses replicate in salivary glands and infect through saliva.

Nature

Ghosh S,Kumar M,Santiana M,Mishra A,Zhang M,Labayo H,Chibly AM,Nakamura H,Tanaka T,Henderson W,Lewis E,Voss O,Su Y,Belkaid Y,Chiorini JA,Hoffman MP,Altan-Bonnet N

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-5791-80) in Flow Cytometry

Fri Jul 01 00:00:00 EDT 2022

IL-33 induces thymic involution-associated naive T cell aging and impairs host control of severe infection.

Nature communications

Xu L,Wei C,Chen Y,Wu Y,Shou X,Chen W,Lu D,Sun H,Li W,Yu B,Wang X,Zhang X,Yu Y,Lei Z,Tang R,Zhu J,Li Y,Lu L,Zhou H,Zhou S,Su C,Chen X

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-5791-80) in Flow Cytometry

Sat Nov 12 00:00:00 EST 2022

A mesenchymal to epithelial switch in Fgf10 expression specifies an evolutionary-conserved population of ionocytes in salivary glands.

Cell reports

Mauduit O,Aure MH,Delcroix V,Basova L,Srivastava A,Umazume T,Mays JW,Bellusci S,Tucker AS,Hajihosseini MK,Hoffman MP,Makarenkova HP

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-5791-80) in Flow Cytometry

Tue Apr 12 00:00:00 EDT 2022

Oncolytic vaccinia virus expressing a bispecific T-cell engager enhances immune responses in EpCAM positive solid tumors.

Frontiers in immunology

Wei M,Zuo S,Chen Z,Qian P,Zhang Y,Kong L,Gao H,Wei J,Dong J

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-5791-80) in Flow Cytometry

Fri Dec 02 00:00:00 EST 2022

A nonredundant role for T cell-derived interleukin 22 in antibacterial defense of colonic crypts.

Immunity

Zindl CL,Witte SJ,Laufer VA,Gao M,Yue Z,Janowski KM,Cai B,Frey BF,Silberger DJ,Harbour SN,Singer JR,Turner H,Lund FE,Vallance BA,Rosenberg AF,Schoeb TR,Chen JY,Hatton RD,Weaver CT

25-5791-80 was used in Immunohistochemistry (Paraffin) to demonstrate spatiotemporal differences in the production and action of IL-22 by ILCs and T cells during infection and reveal an indispensable role for IL-22-producing T cells in the protection of the intestinal crypts.

Tue Mar 08 00:00:00 EST 2022

A nonredundant role for T cell-derived interleukin 22 in antibacterial defense of colonic crypts.

Immunity

Zindl CL,Witte SJ,Laufer VA,Gao M,Yue Z,Janowski KM,Cai B,Frey BF,Silberger DJ,Harbour SN,Singer JR,Turner H,Lund FE,Vallance BA,Rosenberg AF,Schoeb TR,Chen JY,Hatton RD,Weaver CT

Tue Mar 08 00:00:00 EST 2022

Pulmonary neuroendocrine cells sense succinate to stimulate myoepithelial cell contraction.

Developmental cell

Yu W,Moninger TO,Rector MV,Stoltz DA,Welsh MJ

25-5791-80 was used in Immunohistochemistry to identify a local circuit in which rare PNECs within submucosal glands sense an environmental cue to orchestrate the function of airway glands.

Mon Sep 26 00:00:00 EDT 2022

Tumor collection/processing under physioxia uncovers highly relevant signaling networks and drug sensitivity.

Science advances

Kumar B,Adebayo AK,Prasad M,Capitano ML,Wang R,Bhat-Nakshatri P,Anjanappa M,Simpson E,Chen D,Liu Y,Schilder JM,Colter AB,Maguire C,Temm CJ,Sandusky G,Doud EH,Wijeratne AB,Mosley AL,Broxmeyer HE,Nakshatri H

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-5791-80) in Flow Cytometry

Fri Jan 14 00:00:00 EST 2022

Neuroendocrinology of the lung revealed by single-cell RNA sequencing.

eLife

Kuo CS,Darmanis S,Diaz de Arce A,Liu Y,Almanzar N,Wu TT,Quake SR,Krasnow MA

25-5791-80 was used in Cell Culture to show that PNECs are an extraordinarily rich and diverse signaling hub rivaling the enteroendocrine system.

Mon Dec 05 00:00:00 EST 2022

Nuclear respiratory factor-1 negatively regulates TGF-β1 and attenuates pulmonary fibrosis.

iScience

Suliman HB,Healy Z,Zobi F,Kraft BD,Welty-Wolf K,Smith J,Barkauskas C,Piantadosi CA

25-5791-80 was used in Flow cytometry/Cell sorting to show that protection of AT2 cell mitochondrial integrity via HO-1/NRF-1 presents an innovative therapeutic target.

Fri Jan 21 00:00:00 EST 2022

Thymocytes trigger self-antigen-controlling pathways in immature medullary thymic epithelial stages.

eLife

Lopes N,Boucherit N,Santamaria JC,Provin N,Charaix J,Ferrier P,Giraud M,Irla M

25-5791-80 was used in Flow cytometry/Cell sorting to reveal that self-reactive CD4+ thymocytes control multiple unsuspected facets from immature stages of mTECs, which determines their heterogeneity.

Mon Feb 21 00:00:00 EST 2022

Cells of the human intestinal tract mapped across space and time.

Nature

Elmentaite R,Kumasaka N,Roberts K,Fleming A,Dann E,King HW,Kleshchevnikov V,Dabrowska M,Pritchard S,Bolt L,Vieira SF,Mamanova L,Huang N,Perrone F,Goh Kai'En I,Lisgo SN,Katan M,Leonard S,Oliver TRW,Hook CE,Nayak K,Campos LS,Domínguez Conde C,Stephenson E,Engelbert J,Botting RA,Polanski K,van Dongen S,Patel M,Morgan MD,Marioni JC,Bayraktar OA,Meyer KB,He X,Barker RA,Uhlig HH,Mahbubani KT,Saeb-Parsy K,Zilbauer M,Clatworthy MR,Haniffa M,James KR,Teichmann SA

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-5791-80) in Flow Cytometry

Wed Sep 01 00:00:00 EDT 2021

Dynamic cell contacts between periportal mesenchyme and ductal epithelium act as a rheostat for liver cell proliferation.

Cell stem cell

Cordero-Espinoza L,Dowbaj AM,Kohler TN,Strauss B,Sarlidou O,Belenguer G,Pacini C,Martins NP,Dobie R,Wilson-Kanamori JR,Butler R,Prior N,Serup P,Jug F,Henderson NC,Hollfelder F,Huch M

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-5791-80) in Flow Cytometry

Thu Nov 04 00:00:00 EDT 2021

Gut epithelial IL-27 confers intestinal immunity through the induction of intraepithelial lymphocytes.

The Journal of experimental medicine

Lin CH,Chen MC,Lin LL,Christian DA,Min B,Hunter CA,Lu LF

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-5791-80) in Flow Cytometry

Mon Nov 01 00:00:00 EDT 2021

Plant-derived exosomal microRNAs inhibit lung inflammation induced by exosomes SARS-CoV-2 Nsp12.

Molecular therapy : the journal of the American Society of Gene Therapy

Teng Y,Xu F,Zhang X,Mu J,Sayed M,Hu X,Lei C,Sriwastva M,Kumar A,Sundaram K,Zhang L,Park JW,Chen SY,Zhang S,Yan J,Merchant ML,Zhang X,McClain CJ,Wolfe JK,Adcock RS,Chung D,Palmer KE,Zhang HG

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-5791-80) in Flow Cytometry

Wed Aug 04 00:00:00 EDT 2021

Progastrin production transitions from Bmi1⁺/Prox1⁺ to Lgr5^high cells during early intestinal tumorigenesis.

Translational oncology

Giraud J,Foroutan M,Boubaker-Vitre J,Grillet F,Homayed Z,Jadhav U,Crespy P,Breuker C,Bourgaux JF,Hazerbroucq J,Pignodel C,Brulin B,Shivdasani RA,Jay P,Hollande F,Pannequin J

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-5791-80) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

miR-802 regulates Paneth cell function and enterocyte differentiation in the mouse small intestine.

Nature communications

Goga A,Yagabasan B,Herrmanns K,Godbersen S,Silva PN,Denzler R,Zünd M,Furter M,Schwank G,Sunagawa S,Hardt WD,Stoffel M

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-5791-80) in Flow Cytometry

Mon Jun 07 00:00:00 EDT 2021

IGF1R controls mechanosignaling in myofibroblasts required for pulmonary alveologenesis.

JCI insight

He H,Snowball J,Sun F,Na CL,Whitsett JA

25-5791-80 was used in Flow cytometry/Cell sorting to demonstrate that IGF1 receptor gene (Igf1r) expression by a subset of pulmonary fibroblasts is required for normal alveologenesis in mice.

Mon Mar 22 00:00:00 EDT 2021

Isolation and culture of mouse alveolar type II cells to study type II to type I cell differentiation.

STAR protocols

Chen Q,Liu Y

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-5791-80) in Flow Cytometry

Fri Mar 19 00:00:00 EDT 2021

Evolution of fibroblasts in the lung metastatic microenvironment is driven by stage-specific transcriptional plasticity.

eLife

Shani O,Raz Y,Monteran L,Scharff Y,Levi-Galibov O,Megides O,Shacham H,Cohen N,Silverbush D,Avivi C,Sharan R,Madi A,Scherz-Shouval R,Barshack I,Tsarfaty I,Erez N

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-5791-80) in Flow Cytometry

Fri Jun 25 00:00:00 EDT 2021

Establishment of a fluorescent reporter of RNA-polymerase II activity to identify dormant cells.

Nature communications

Freter R,Falletta P,Omrani O,Rasa M,Herbert K,Annunziata F,Minetti A,Krepelova A,Adam L,Käppel S,Rüdiger T,Wang ZQ,Goding CR,Neri F

25-5791-80 was used in Flow Cytometry to identify a dormant OSCARhigh cell population in the small intestine.

Thu Jun 03 00:00:00 EDT 2021

Lef1 restricts ectopic crypt formation and tumor cell growth in intestinal adenomas.

Science advances

Heino S,Fang S,Lähde M,Högström J,Nassiri S,Campbell A,Flanagan D,Raven A,Hodder M,Nasreddin N,Xue HH,Delorenzi M,Leedham S,Petrova TV,Sansom O,Alitalo K

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-5791-80) in Flow Cytometry

Fri Nov 19 00:00:00 EST 2021

Serum Amyloid A Proteins Induce Pathogenic Th17 Cells and Promote Inflammatory Disease.

Cell

Lee JY,Hall JA,Kroehling L,Wu L,Najar T,Nguyen HH,Lin WY,Yeung ST,Silva HM,Li D,Hine A,Loke P,Hudesman D,Martin JC,Kenigsberg E,Merad M,Khanna KM,Littman DR

25-5791 was used in Flow cytometry/Cell sorting to suggest that serum amyloid A proteins direct a pathogenic pro-inflammatory Th17 cell differentiation program and promote Th17-mediated inflammatory diseases.

Thu Jan 09 00:00:00 EST 2020

Comparative analysis of cell lineage differentiation during hepatogenesis in humans and mice at the single-cell transcriptome level.

Cell research

Wang X,Yang L,Wang YC,Xu ZR,Feng Y,Zhang J,Wang Y,Xu CR

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-5791-80) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2020

Legionella-Infected Macrophages Engage the Alveolar Epithelium to Metabolically Reprogram Myeloid Cells and Promote Antibacterial Inflammation.

Cell host & microbe

Liu X,Boyer MA,Holmgren AM,Shin S

25-5791-80 was used in Flow Cytometry to reveal that alveolar macrophages engage alveolar epithelial signals to metabolically reprogram monocytes for antibacterial inflammation.

Wed Nov 11 00:00:00 EST 2020

Transfer of metastatic traits via miR-200c in extracellular vesicles derived from colorectal cancer stem cells is inhibited by atractylenolide I.

Clinical and translational medicine

Tang D,Xu X,Ying J,Xie T,Cao G

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-5791-80) in Flow Cytometry

Sat Aug 01 00:00:00 EDT 2020

Impact of a high‑fat diet on intestinal stem cells and epithelial barrier function in middle‑aged female mice.

Molecular medicine reports

Xie Y,Ding F,Di W,Lv Y,Xia F,Sheng Y,Yu J,Ding G

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-5791-80) in Flow Cytometry

Sun Mar 01 00:00:00 EST 2020

Smarcad1 mediates microbiota-induced inflammation in mouse and coordinates gene expression in the intestinal epithelium.

Genome biology

Kazakevych J,Denizot J,Liebert A,Portovedo M,Mosavie M,Jain P,Stellato C,Fraser C,Corrêa RO,Célestine M,Mattiuz R,Okkenhaug H,Miller JR,Vinolo MAR,Veldhoen M,Varga-Weisz P

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-5791-80) in Flow Cytometry

Wed Mar 11 00:00:00 EDT 2020

Dietary Glucose Consumption Promotes RALDH Activity in Small Intestinal CD103⁺CD11b⁺ Dendritic Cells.

Frontiers in immunology

Ko HJ,Hong SW,Verma R,Jung J,Lee M,Kim N,Kim D,Surh CD,Kim KS,Rudra D,Im SH

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-5791-80) in Flow Cytometry

Mon Apr 19 00:00:00 EDT 2021

Rare Pulmonary Neuroendocrine Cells Are Stem Cells Regulated by Rb, p53, and Notch.

Cell

Ouadah Y,Rojas ER,Riordan DP,Capostagno S,Kuo CS,Krasnow MA

25-5791 was used in Flow cytometry/Cell sorting to propose that neuroendocrine stem cells give rise to small cell lung cancer , and transformation results from constitutive activation of stem cell renewal and inhibition of deprogramming.

Thu Oct 03 00:00:00 EDT 2019

Logic-Gated ROR1 Chimeric Antigen Receptor Expression Rescues T Cell-Mediated Toxicity to Normal Tissues and Enables Selective Tumor Targeting.

Cancer cell

Srivastava S,Salter AI,Liggitt D,Yechan-Gunja S,Sarvothama M,Cooper K,Smythe KS,Dudakov JA,Pierce RH,Rader C,Riddell SR

25-5791 was used in Flow cytometry/Cell sorting to demonstrate the use of “AND” logic gates as a strategy to increase the portfolio of cell surface molecules on solid tumors that can be targeted safely with CAR-T cells.

Mon Mar 18 00:00:00 EDT 2019

The MMTV-Wnt1 murine model produces two phenotypically distinct subtypes of mammary tumors with unique therapeutic responses to an EGFR inhibitor.

Disease models & mechanisms

Pfefferle AD,Darr DB,Calhoun BC,Mott KR,Rosen JM,Perou CM

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-5791-80) in Flow Cytometry

Fri Jul 05 00:00:00 EDT 2019

Cellular Plasticity of Defa4^Cre-Expressing Paneth Cells in Response to Notch Activation and Intestinal Injury.

Cellular and molecular gastroenterology and hepatology

Jones JC,Brindley CD,Elder NH,Myers MG,Rajala MW,Dekaney CM,McNamee EN,Frey MR,Shroyer NF,Dempsey PJ

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-5791-80) in Flow Cytometry

Tue Jun 04 00:00:00 EDT 2019

Procr-expressing progenitor cells are responsible for murine ovulatory rupture repair of ovarian surface epithelium.

Nature communications

Wang J,Wang D,Chu K,Li W,Zeng YA

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-5791-80) in Flow Cytometry

Thu Oct 31 00:00:00 EDT 2019

Potent Antitumor Activity of Liposomal Irinotecan in an Organoid- and CRISPR-Cas9-Based Murine Model of Gallbladder Cancer.

Cancers

Erlangga Z,Wolff K,Poth T,Peltzer A,Nahnsen S,Spielberg S,Timrott K,Woller N,Kühnel F,Manns MP,Saborowski A,Vogel A,Saborowski M

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-5791-80) in Flow Cytometry

Fri Nov 29 00:00:00 EST 2019

Murine Liver Organoids as a Genetically Flexible System to Study Liver Cancer In Vivo and In Vitro.

Hepatology communications

Saborowski A,Wolff K,Spielberg S,Beer B,Hartleben B,Erlangga Z,Becker D,Dow LE,Marhenke S,Woller N,Unger K,Schirmacher P,Manns MP,Marquardt JU,Vogel A,Saborowski M

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-5791-80) in Flow Cytometry

Fri Mar 01 00:00:00 EST 2019

DC3 is a method for deconvolution and coupled clustering from bulk and single-cell genomics data.

Nature communications

Zeng W,Chen X,Duren Z,Wang Y,Jiang R,Wong WH

25-5791-80 was used in Flow cytometry/Cell sorting to show that the subpopulation-specific profiles of gene expression, chromatin accessibility and enhancer-promoter contact obtained by DC3 provide a comprehensive characterization of the gene regulatory system in each subpopulation.

Thu Oct 10 00:00:00 EDT 2019

Microbiota-derived acetate protects against respiratory syncytial virus infection through a GPR43-type 1 interferon response.

Nature communications

Antunes KH,Fachi JL,de Paula R,da Silva EF,Pral LP,Dos Santos AÁ,Dias GBM,Vargas JE,Puga R,Mayer FQ,Maito F,Zárate-Bladés CR,Ajami NJ,Sant'Ana MR,Candreva T,Rodrigues HG,Schmiele M,Silva Clerici MTP,Proença-Modena JL,Vieira AT,Mackay CR,Mansur D,Caballero MT,Marzec J,Li J,Wang X,Bell D,Polack FP,Kleeberger SR,Stein RT,Vinolo MAR,de Souza APD

25-5791-80 was used in Flow Cytometry to reveal antiviral effects of acetate involving IFN-β in lung epithelial cells and engagement of GPR43 and IFNAR.

Mon Jul 22 00:00:00 EDT 2019

A revised airway epithelial hierarchy includes CFTR-expressing ionocytes.

Nature

Montoro DT,Haber AL,Biton M,Vinarsky V,Lin B,Birket SE,Yuan F,Chen S,Leung HM,Villoria J,Rogel N,Burgin G,Tsankov AM,Waghray A,Slyper M,Waldman J,Nguyen L,Dionne D,Rozenblatt-Rosen O,Tata PR,Mou H,Shivaraju M,Bihler H,Mense M,Tearney GJ,Rowe SM,Engelhardt JF,Regev A,Rajagopal J

25-5791-80 was used in Flow Cytometry to study the cellular composition and hierarchy of the murine tracheal epithelium.

Wed Aug 01 00:00:00 EDT 2018

Lung epithelial cell-derived IL-25 negatively regulates LPS-induced exosome release from macrophages.

Military Medical Research

Li ZG,Scott MJ,Brzóska T,Sundd P,Li YH,Billiar TR,Wilson MA,Wang P,Fan J

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-5791-80) in Flow Cytometry

Mon Jul 30 00:00:00 EDT 2018

YAP/TAZ-Dependent Reprogramming of Colonic Epithelium Links ECM Remodeling to Tissue Regeneration.

Cell stem cell

Yui S,Azzolin L,Maimets M,Pedersen MT,Fordham RP,Hansen SL,Larsen HL,Guiu J,Alves MRP,Rundsten CF,Johansen JV,Li Y,Madsen CD,Nakamura T,Watanabe M,Nielsen OH,Schweiger PJ,Piccolo S,Jensen KB

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-5791-80) in Flow Cytometry

Thu Jan 04 00:00:00 EST 2018

Lymphotoxin α fine-tunes T cell clonal deletion by regulating thymic entry of antigen-presenting cells.

Nature communications

Lopes N,Charaix J,Cédile O,Sergé A,Irla M

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-5791-80) in Flow Cytometry

Wed Mar 28 00:00:00 EDT 2018

Molecular Characterization of Gastric Epithelial Cells Using Flow Cytometry.

International journal of molecular sciences

Bockerstett KA,Wong CF,Koehm S,Ford EL,DiPaolo RJ

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-5791-80) in Flow Cytometry

Fri Apr 06 00:00:00 EDT 2018

Differential requirement of kindlin-3 for T cell progenitor homing to the non-vascularized and vascularized thymus.

eLife

Moretti FA,Klapproth S,Ruppert R,Margraf A,Weber J,Pick R,Scheiermann C,Sperandio M,Fässler R,Moser M

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-5791-80) in Flow Cytometry

Thu Sep 06 00:00:00 EDT 2018

ATF3 Sustains IL-22-Induced STAT3 Phosphorylation to Maintain Mucosal Immunity Through Inhibiting Phosphatases.

Frontiers in immunology

Glal D,Sudhakar JN,Lu HH,Liu MC,Chiang HY,Liu YC,Cheng CF,Shui JW

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-5791-80) in Flow Cytometry

Thu Sep 26 00:00:00 EDT 2019

Single-Cell RNA-Seq Reveals Dynamic Early Embryonic-like Programs during Chemical Reprogramming.

Cell stem cell

Zhao T,Fu Y,Zhu J,Liu Y,Zhang Q,Yi Z,Chen S,Jiao Z,Xu X,Xu J,Duo S,Bai Y,Tang C,Li C,Deng H

25-5791 was used in Flow cytometry/Cell sorting to profile 36,199 single-cell transcriptomes to reveal interactions during a highly efficient chemical reprogramming system.

Thu Jul 05 00:00:00 EDT 2018

DND1 maintains germline stem cells via recruitment of the CCR4-NOT complex to target mRNAs.

Nature

Yamaji M,Jishage M,Meyer C,Suryawanshi H,Der E,Yamaji M,Garzia A,Morozov P,Manickavel S,McFarland HL,Roeder RG,Hafner M,Tuschl T

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-5791-80) in Flow Cytometry

Thu Mar 23 00:00:00 EDT 2017

Effects of Orally Ingested Arsenic on Respiratory Epithelial Permeability to Bacteria and Small Molecules in Mice.

Environmental health perspectives

Henderson MW,Madenspacher JH,Whitehead GS,Thomas SY,Aloor JJ,Gowdy KM,Fessler MB

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-5791-80) in Flow Cytometry

Thu Sep 28 00:00:00 EDT 2017

An essential role of CBL and CBL-B ubiquitin ligases in mammary stem cell maintenance.

Development (Cambridge, England)

Mohapatra B,Zutshi N,An W,Goetz B,Arya P,Bielecki TA,Mushtaq I,Storck MD,Meza JL,Band V,Band H

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-5791-80) in Flow Cytometry

Wed Mar 15 00:00:00 EDT 2017

ABCG1 regulates pulmonary surfactant metabolism in mice and men.

Journal of lipid research

de Aguiar Vallim TQ,Lee E,Merriott DJ,Goulbourne CN,Cheng J,Cheng A,Gonen A,Allen RM,Palladino END,Ford DA,Wang T,Baldán Á,Tarling EJ

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-5791-80) in Flow Cytometry

Mon May 01 00:00:00 EDT 2017

The C-terminus domain of the hepatitis B virus x protein stimulates the proliferation of mouse foetal hepatic progenitor cells, although it is not required for the formation of spheroids.

International journal of molecular medicine

Yu L,Chen S,Luo N,He S

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-5791-80) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2017

The role of TGFβ‑HGF‑Smad4 axis in regulating the proliferation of mouse airway progenitor cells.

Molecular medicine reports

Li X,Yang L,Sun X,Wu J,Li Y,Zhang Q,Zhang Y,Li K,Wu Q,Chen H

25-5791-80 was used in Flow cytometry/Cell sorting to suggest a role for the TGF‚β‚TGF‚βR1/2‚HGF‚Smad4 axis in airway epithelial homeostasis.

Fri Dec 01 00:00:00 EST 2017

Reconstructing Lineage Hierarchies of Mouse Uterus Epithelial Development Using Single-Cell Analysis.

Stem cell reports

Wu B,An C,Li Y,Yin Z,Gong L,Li Z,Liu Y,Heng BC,Zhang D,Ouyang H,Zou X

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-5791-80) in Flow Cytometry

Tue Jul 11 00:00:00 EDT 2017

Guanine nucleotide exchange factor RABGEF1 regulates keratinocyte-intrinsic signaling to maintain skin homeostasis.

The Journal of clinical investigation

Marichal T,Gaudenzio N,El Abbas S,Sibilano R,Zurek O,Starkl P,Reber LL,Pirottin D,Kim J,Chambon P,Roers A,Antoine N,Kawakami Y,Kawakami T,Bureau F,Tam SY,Tsai M,Galli SJ

25579180 was used in flow cytometry to show that deletion of the gene encoding RAB guanine nucleotide exchange factor 1 in keratinocytes severely impairs epidermal barrier function in mice

Thu Dec 01 00:00:00 EST 2016

Circulating tumor cell detection in hepatocellular carcinoma based on karyoplasmic ratios using imaging flow cytometry.

Scientific reports

Liu Z,Guo W,Zhang D,Pang Y,Shi J,Wan S,Cheng K,Wang J,Cheng S

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-5791-80) in Flow Cytometry

Fri Dec 23 00:00:00 EST 2016

Regulation of Prostate Development and Benign Prostatic Hyperplasia by Autocrine Cholinergic Signaling via Maintaining the Epithelial Progenitor Cells in Proliferating Status.

Stem cell reports

Wang N,Dong BJ,Quan Y,Chen Q,Chu M,Xu J,Xue W,Huang YR,Yang R,Gao WQ

25-5791 was used in Flow cytometry/Cell sorting to investigate the role of autocrine cholinergic signaling (ASC) in prostate development, showing that development is regulated by ASC.

Tue May 10 00:00:00 EDT 2016

Long-Term In Vitro Expansion of Salivary Gland Stem Cells Driven by Wnt Signals.

Stem cell reports

Maimets M,Rocchi C,Bron R,Pringle S,Kuipers J,Giepmans BN,Vries RG,Clevers H,de Haan G,van Os R,Coppes RP

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-5791-80) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2016

LTβR controls thymic portal endothelial cells for haematopoietic progenitor cell homing and T-cell regeneration.

Nature communications

Shi Y,Wu W,Chai Q,Li Q,Hou Y,Xia H,Ren B,Xu H,Guo X,Jin C,Lv M,Wang Z,Fu YX,Zhu M

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-5791-80) in Flow Cytometry

Fri Aug 05 00:00:00 EDT 2016

A Serial shRNA Screen for Roadblocks to Reprogramming Identifies the Protein Modifier SUMO2.

Stem cell reports

Borkent M,Bennett BD,Lackford B,Bar-Nur O,Brumbaugh J,Wang L,Du Y,Fargo DC,Apostolou E,Cheloufi S,Maherali N,Elledge SJ,Hu G,Hochedlinger K

25-5791 was used in Flow cytometry/Cell sorting to identify potent repressors of somatic cell reprogramming into induced pluripotent stem cells.

Tue May 10 00:00:00 EDT 2016

Parent stem cells can serve as niches for their daughter cells.

Nature

Pardo-Saganta A,Tata PR,Law BM,Saez B,Chow RD,Prabhu M,Gridley T,Rajagopal J

25-5791 was used in Flow cytometry/Cell sorting to describe a signalling relay from adult mammalian progenitor cells and reveal it to be necessary for daughter cell maintenance.

Thu Jul 30 00:00:00 EDT 2015

Glycoprotein A33 deficiency: a new mouse model of impaired intestinal epithelial barrier function and inflammatory disease.

Disease models & mechanisms

Williams BB,Tebbutt NC,Buchert M,Putoczki TL,Doggett K,Bao S,Johnstone CN,Masson F,Hollande F,Burgess AW,Scott AM,Ernst M,Heath JK

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-5791-80) in Flow Cytometry

Sat Aug 01 00:00:00 EDT 2015

CARMA3 Is Critical for the Initiation of Allergic Airway Inflammation.

Journal of immunology (Baltimore, Md. : 1950)

Causton B,Ramadas RA,Cho JL,Jones K,Pardo-Saganta A,Rajagopal J,Xavier RJ,Medoff BD

25-5791 was used in Flow cytometry/Cell sorting to show that CARMA3 is a critical signalling molecule bridging the innate and adaptive immune responses in the lung.

Wed Jul 15 00:00:00 EDT 2015

Low- and high-LET radiation drives clonal expansion of lung progenitor cells in vivo.

Radiation research

Farin AM,Manzo ND,Kirsch DG,Stripp BR

25-5791 was used in Flow cytometry/Cell sorting to propose that radiation induces a dose-dependent decrease in progenitor cells, leaving fewer progenitors able to maintain the airway long-term.

Thu Jan 01 00:00:00 EST 2015

Live fibroblast harvest reveals surface marker shift in vitro.

Tissue engineering. Part C, Methods

Walmsley GG,Rinkevich Y,Hu MS,Montoro DT,Lo DD,McArdle A,Maan ZN,Morrison SD,Duscher D,Whittam AJ,Wong VW,Weissman IL,Gurtner GC,Longaker MT

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-5791-80) in Flow Cytometry

Sun Mar 01 00:00:00 EST 2015

Immune complexes stimulate CCR7-dependent dendritic cell migration to lymph nodes.

Nature medicine

Clatworthy MR,Aronin CE,Mathews RJ,Morgan NY,Smith KG,Germain RN

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-5791-80) in Flow Cytometry

Mon Dec 01 00:00:00 EST 2014

Identification of novel thymic epithelial cell subsets whose differentiation is regulated by RANKL and Traf6.

PloS one

Danzl NM,Jeong S,Choi Y,Alexandropoulos K

25-5791 was used in Flow cytometry/Cell sorting to examine the developmental stages of thymic epithelial cells, identifying novel thymic epithelial cell subsets whose differentiation is regulated by RANKL and Traf6.

Wed Oct 29 00:00:00 EDT 2014

Dedifferentiation of committed epithelial cells into stem cells in vivo.

Nature

Tata PR,Mou H,Pardo-Saganta A,Zhao R,Prabhu M,Law BM,Vinarsky V,Cho JL,Breton S,Sahay A,Medoff BD,Rajagopal J

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-5791-80) in Flow Cytometry

Thu Nov 14 00:00:00 EST 2013

KDR identifies a conserved human and murine hepatic progenitor and instructs early liver development.

Cell stem cell

Goldman O,Han S,Sourisseau M,Dziedzic N,Hamou W,Corneo B,D'Souza S,Sato T,Kotton DN,Bissig KD,Kalir T,Jacobs A,Evans T,Evans MJ,Gouon-Evans V

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-5791-80) in Flow Cytometry

Thu Jun 06 00:00:00 EDT 2013

Type 2 alveolar cells are stem cells in adult lung.

The Journal of clinical investigation

Barkauskas CE,Cronce MJ,Rackley CR,Bowie EJ,Keene DR,Stripp BR,Randell SH,Noble PW,Hogan BL

25-5791 was used in Flow cytometry/Cell sorting to obtain new information about the contribution of epithelial cells to alveolar maintenance and repair.

Mon Jul 01 00:00:00 EDT 2013

A multicenter study to standardize reporting and analyses of fluorescence-activated cell-sorted murine intestinal epithelial cells.

American journal of physiology. Gastrointestinal and liver physiology

Magness ST,Puthoff BJ,Crissey MA,Dunn J,Henning SJ,Houchen C,Kaddis JS,Kuo CJ,Li L,Lynch J,Martin MG,May R,Niland JC,Olack B,Qian D,Stelzner M,Swain JR,Wang F,Wang J,Wang X,Yan K,Yu J,Wong MH

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-5791-80) in Flow Cytometry

Tue Oct 15 00:00:00 EDT 2013

Gli1 deletion prevents Helicobacter-induced gastric metaplasia and expansion of myeloid cell subsets.

PloS one

El-Zaatari M,Kao JY,Tessier A,Bai L,Hayes MM,Fontaine C,Eaton KA,Merchant JL

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-5791-80) in Flow Cytometry

Tue Sep 10 00:00:00 EDT 2013

Smad1 signaling restricts hematopoietic potential after promoting hemangioblast commitment.

Blood

Cook BD,Liu S,Evans T

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-5791-80) in Flow Cytometry

Thu Jun 16 00:00:00 EDT 2011

Epidermal ablation of Dlx3 is linked to IL-17-associated skin inflammation.

Proceedings of the National Academy of Sciences of the United States of America

Hwang J,Kita R,Kwon HS,Choi EH,Lee SH,Udey MC,Morasso MI

25-5791 was used in Flow cytometry/Cell sorting to investigate the role of Distal-less 3 (Dlx3) in cutaneous biology and pathophysiology, showing that ablation is linked to IL-17-associated skin inflammation.

Tue Jul 12 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: CD326; EGP314; Ep-CAM; Epithelial cell adhesion molecule; Epithelial glycoprotein 314; hEGP314; lymphocyte antigen 74; mEGP314; panepithelial glycoprotein 314; Protein 289A; Trop-1 protein; Tumor-associated calcium signal transducer 1

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Gene Aliases: CD326; EGP; EGP-2; Egp314; Ep-CAM; Epcam; EpCAM1; GA733-2; gp40; Ly74; Tacsd1; Tacstd1; TROP1

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UniProt ID: (Mouse) Q99JW5

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Entrez Gene ID: (Mouse) 17075

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Function(s)

protein complex binding cadherin binding involved in cell-cell adhesion

Process(es)

positive regulation of cell proliferation signal transduction involved in regulation of gene expression negative regulation of apoptotic process positive regulation of transcription from RNA polymerase II promoter stem cell differentiation cell-cell adhesion negative regulation of cell-cell adhesion mediated by cadherin positive regulation of cell motility positive regulation of stem cell proliferation

Performance Guarantee

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CD326 (EpCAM) Monoclonal Antibody (G8.8), PE-Cyanine7, eBioscience™

CD326 (EpCAM) Antibody (25-5791-80) in Flow

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25-5791-80

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