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Invitrogen
IgD Monoclonal Antibody (11-26c (11-26)), Super Bright™ 436, eBioscience™
FIGURE: 1 / 3
IgD Antibody (62-5993-82) in Flow
C57BL/6 mouse splenocytes were stained with CD45R (B220) Monoclonal Antibody, APC (Product # 17-0452-82) and 0.25 µg of Rat IgG2a kappa Isotype Control, Super Bright 436 (Product # 62-4321-82) (left) or 0.25 µg of IgD Monoclonal Antibody, Super Bright 436 (right). Cells in the lymphocyte gate were used for analysis.
Product Details
62-5993-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Not Applicable
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
11-26c (11-26)
Conjugate
Excitation/Emission Max
413/431 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2762759
Product Specific Information
Description: The 11-26c monoclonal antibody reacts with the delta heavy chain of mouse IgD. It does not react with other classes of mouse immunoglobulin including IgA, IgG or IgM. IgD is expressed by peripheral mature B cells. 11-26c does not activate B cells.
Applications Reported: This 11-26 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 11-26 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.
When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.
Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
This type I transmembrane protein is co-expressed on the surface of mature naive B cells with membrane IgM. IgD also exists as a soluble form in blood serum. Studies have demonstrated that IgD associates with the B cell receptor and participates in mediating signal transduction upon antigen binding.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Gene Aliases: IgD; Igh-5
Entrez Gene ID: (Mouse) 380797
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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