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ACAP2 Antibody (PA5-114808) in ICC/IF
Immunocytochemistry analysis of ACAP2 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-114808) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Product Details
PA5-114808
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human ACAP2(Accession Q15057), corresponding to amino acid residues G746-F778.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2899444
Product Specific Information
Antibody detects endogenous levels of total ACAP2.
Target Information
ACAP2 is a transport carrier belonging to the Centaurin family with three ANK repeats, an Arf-GAP domain, a BAR domain, and a PH domain. ACAP2, a GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) has robust constitutive ARF6 GAP activity, where the GAP activity is stimulated by phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidic acid but has little activity toward ARF1 and is known to control the return of ARF to the inactive GDB-bound shots. ACAP2, along with ACAP1, is targeted by phosphatidyl inositol (3, 4, 3) triphosphate inhibiting the formation of PDGF-induced dorsal membrane ruffles in NIH3T3 fibroblasts when overexpressed. ACAP2 is phosphorylated upon DNA damage, probably by ATM or ATR. Expression is wide-spread with the highest level in lung.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Arf GAP with coiled coil, ANK repeat and PH domains 2; Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2; centaurin beta2; centaurin, beta 2; Centaurin-beta-2; Cnt-b2
Gene Aliases: 4832442G16; 9530039J15Rik; ACAP2; CENTB2; CNT-B2; KIAA0041; mKIAA0041
UniProt ID: (Human) Q15057, (Mouse) Q6ZQK5, (Rat) Q5FVC7
Entrez Gene ID: (Human) 23527, (Mouse) 78618, (Rat) 619382
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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