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ACAT2 Antibody (MA5-42668) in WB
Western blot analysis of ACAT2 in extracts of various cell lines. Samples were incubated with ACAT2 Monoclonal antibody (Product # MA5-42668) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
MA5-42668
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
6Z5L1
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 150-250 of human ACAT2 (Q9BWD1).
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2911809
Product Specific Information
Positive test controls include: HeLa, SW620, HepG2, Mouse liver, Mouse brain, Mouse kidney, Rat brain, Rat kidney. The target is usually found in the following locations: Cytoplasm.
Immunogen sequence: GLTDAFHNCH MGITAENVAK KWQVSREDQD KVAVLSQNRT ENAQKAGHFD KEIVPVLVST RKGLIEVKTD EFPRHGSNIE AMSKLKPYFL TDGTGTVTPA N
Target Information
This gene represents a member of the 'frizzled' gene family, which encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The protein encoded by this family member contains a signal peptide, a cysteine-rich domain in the N-terminal extracellular region, and seven transmembrane domains, but unlike other family members, this protein does not contain a C-terminal PDZ domain-binding motif. This protein functions as a negative regulator of the canonical Wnt/beta-catenin signaling cascade, thereby inhibiting the processes that trigger oncogenic transformation, cell proliferation, and inhibition of apoptosis. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: acetoacetyl Coenzyme A thiolase; Acetyl-CoA acetyltransferase, cytosolic; Acetyl-CoA transferase-like protein; acetyl-Coenzyme A acetyltransferase 2; acetyl-Coenzyme A acetyltransferase 3; AE1; BND3; Cytosolic acetoacetyl-CoA thiolase; DI; EMPB3; EPB3; RTA1A; SW; t-complex protein 1, related sequence 1; WD; WD1; WR
Gene Aliases: Ab2-076; ACAT2; Acat3; ACTL; AW742799; Tcp-1x; Tcp1-rs1
UniProt ID: (Human) Q9BWD1, (Rat) Q5XI22, (Mouse) Q8CAY6
Entrez Gene ID: (Human) 39, (Rat) 308100, (Mouse) 110460
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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