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AMID Antibody (MA5-51255) in IHC (P)
Immunohistochemistry analysis of AMID in paraffin-embedded rat kidney. Samples were incubated with AMID Recombinant monoclonal antibody (Product # MA5-51255) using a dilution of 1:300 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Product Details
MA5-51255
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
7X9B2
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 43-371 of human AIFM2/FSP1 (NP_116186.1).
Conjugate
Form
Liquid
Concentration
0.25 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_3093789
Product Specific Information
Immunogen sequence: KDSFHHNVAA LRASVETGFA KKTFISYSVT FKDNFRQGLV VGIDLKNQMV LLQGGEALPF SHLILATGST GPFPGKFNEV SSQQAAIQAY EDMVRQVQRS RFIVVVGGGS AGVEMAAEIK TEYPEKEVTL IHSQVALADK ELLPSVRQEV KEILLRKGVQ LLLSERVSNL EELPLNEYRE YIKVQTDKGT EVATNLVILC TGIKINSSAY RKAFESRLAS SGALRVNEHL QVEGHSNVYA IGDCADVRTP KMAYLAGLHA NIAVANIVNS VKQRPLQAYK PGALTFLLSM GRNDGVGQIS GFYVGRLMVR LTKSRDLFVS TSWKTMRQS
Target Information
The p53 tumor suppressor protein induces cell cycle arrest or apoptosis in response to cellular stresses. PRG3 (p53-responsive gene 3), is induced specifically under p53-dependent apoptotic conditions in human colon cancer cells. PRG3 has significant homology to bacterial oxidoreductases and the apoptosis-inducing factor, AIF. Unlike AIF, PRG3 localizes in the cytoplasm and its ectopic expression induces apoptosis. The PRG3 gene is thus a novel p53 target gene in a p53-dependent apoptosis pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AMID; apoptosis-inducing factor (AIF)-homologous mitochondrion-associated inducer of death; apoptosis-inducing factor (AIF)-like mitochondrion-associated inducer of death; apoptosis-inducing factor 2; Apoptosis-inducing factor homologous mitochondrion-associated inducer of death; apoptosis-inducing factor, mitochondrion-associated, 2; apoptosis-inducing factor-like mitchondrion-associated inducer of death; apoptosis-inducing factor-like mitochondrion-associated inducer of death; Ferroptosis suppressor protein 1; FSP1; MBP2; MBPH; MGC126662; MGC141971; p53-responsive gene 3 protein; RP11-367H5.2
Gene Aliases: 5430437E11Rik; AIFM2; AMID; D730001I10Rik; PRG3
UniProt ID: (Human) Q9BRQ8, (Mouse) Q8BUE4
Entrez Gene ID: (Human) 84883, (Mouse) 71361, (Rat) 361843
DNA binding
electron-transferring-flavoprotein dehydrogenase activity
flavin adenine dinucleotide binding
NADH dehydrogenase (ubiquinone) activity
N-ethylmaleimide reductase activity
oxidoreductase activity
reduced coenzyme F420 dehydrogenase activity
sulfur oxygenase reductase activity
malolactic enzyme activity
NADPH:sulfur oxidoreductase activity
epoxyqueuosine reductase activity
oxidoreductase
metabolite interconversion enzyme
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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