Invitrogen

AMPK alpha-1 Monoclonal Antibody (2B7)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

3 Published Figures

6 References

View all (72) AMPK alpha-1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

AMPK alpha-1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 11

AMPK alpha-1 Antibody (MA5-15815) in ICC/IF

Immunofluorescence analysis of AMPK alpha-1 was performed using 70% confluent log phase LNCap cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with AMPK alpha-1 Monoclonal Antibody (2B7)(Product # MA5-15815) at 1:100 dilution in 0.1% BSA and incubated overn... View More

AMPK alpha-1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

AMPK alpha-1 Antibody in Western Blot (WB)

AMPK alpha-1 Antibody in Flow Cytometry (Flow)

Product Image Gallery

Product Details

MA5-15815

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

Immunocytochemistry (ICC/IF)

1:200-1:1,000

Flow Cytometry (Flow)

1:200-1:400

ELISA (ELISA)

1:10,000

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2B7

Immunogen

Purified recombinant fragment of human PRKAA1 expressed in E. Coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_11152993

Product Specific Information

MA5-15815 targets PRKAA1 in indirect ELISA, FACS, IF, IHC, and WB applications and shows reactivity with Human, mouse, Non-human primate, and Rat samples.

The MA5-15815 immunogen is purified recombinant fragment of human PRKAA1 expressed in E. Coli.

MA5-15815 detects PRKAA1 which has a predicted molecular weight of approximately 64kDa.

Target Information

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Penta-O-Galloyl-β-D-Glucose in Pistacia integerrima Targets AMPK-ULK1 and ERK/STAT3 Signaling Axes to Induce ROS-Independent Autophagic Cell Death in Human Lung Cancer Cells.

Frontiers in pharmacology

Balkrishna A,Mulay VP,Verma S,Srivastava J,Lochab S,Varshney A

MA5-15815 was used in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to propose PGG as a potential AMPK activator and STAT3 inhibitor that can be exploited further in developing adjuvant chemotherapeutics against lung cancer.

Sat Aug 06 00:00:00 EDT 2022

ACSL4 contributes to sevoflurane-induced ferroptotic neuronal death in SH-SY5Y cells via the 5' AMP-activated protein kinase/mammalian target of rapamycin pathway.

Annals of translational medicine

Cheng L,Zhu X,Liu Y,Zhu K,Lin K,Li F

MA5-15815 was used in Western Blot to show that downregulation of ACSL4 restrained sev-induced ferroptotic cell death via AMPK/mTOR signaling, providing the basis for an approach to alleviate sev-induced postoperative cognitive dysfunction (POCD).

Wed Sep 01 00:00:00 EDT 2021

Antifungal itraconazole ameliorates experimental autoimmune encephalomyelitis through a novel mechanism of action.

Advances in clinical and experimental medicine : official organ Wroclaw Medical University

Huang H,Tian X,Peng X,Huang L,Mei L,Zhan Y,Chen S,Wu H,Wei G,Cai X

MA5-15815 was used in Western Blotting to reveal a new method for treating MS using currently approved antifungal drugs.

Fri May 01 00:00:00 EDT 2020

α1AMP-Activated Protein Kinase Protects against Lipopolysaccharide-Induced Endothelial Barrier Disruption via Junctional Reinforcement and Activation of the p38 MAPK/HSP27 Pathway.

International journal of molecular sciences

Angé M,Castanares-Zapatero D,De Poortere J,Dufeys C,Courtoy GE,Bouzin C,Quarck R,Bertrand L,Beauloye C,Horman S

Published figure using AMPK alpha-1 monoclonal antibody (Product # MA5-15815) in Western Blot

Tue Aug 04 00:00:00 EDT 2020

Rotating magnetic field delays human umbilical vein endothelial cell aging and prolongs the lifespan of Caenorhabditis elegans.

Aging

Xu J,Liu K,Chen T,Zhan T,Ouyang Z,Wang Y,Liu W,Zhang X,Sun Y,Xu G,Wang X

MA5-15815 was used in Western Blotting to investigate the effects of a rotating magnetic field (RMF; 0.2 T, 4 Hz) on the growth of human umbilical vein endothelial cells (HUVECs) and Caenorhabditis elegans.

Fri Nov 22 00:00:00 EST 2019

Phosphorylation of ULK1 by AMPK regulates translocation of ULK1 to mitochondria and mitophagy.

FEBS letters

Tian W,Li W,Chen Y,Yan Z,Huang X,Zhuang H,Zhong W,Chen Y,Wu W,Lin C,Chen H,Hou X,Zhang L,Sui S,Zhao B,Hu Z,Li L,Feng D

MA5-15815 was used in western blot to elucidate the function of ULK1 activation and translocation in mycophagy

Wed Jul 08 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: 5'-AMP-activated protein kinase catalytic subunit alpha-1; 5'-AMP-activated protein kinase, catalytic alpha-1 chain; AAPK2; ACACA kinase; Acetyl-CoA carboxylase kinase; activated protein kinase; AMP -activate kinase alpha 1 subunit; AMP activated protein kinase; AMP-activated protein kinase, alpha 1 catalytic subunit; AMP-activated protein kinase, catalytic, alpha-1; AMPK alpha 1; AMPK alpha-1 chain; AMPK subunit alpha-1; AMPK subunit pAMPK; AMPK-alpha-2 chain; AMPK2; HMGCR kinase; Hydroxymethylglutaryl-CoA reductase kinase; MGC33776; MGC57364; pAMPK; PRKAA; PRKAA2; protein kinase, AMP-activated, alpha 1 catalytic subunit; Tau-protein kinase PRKAA1

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Gene Aliases: AI194361; AI450832; AL024255; AMPK; AMPK1; AMPKa1; AMPKalpha1; C130083N04Rik; PRKAA1

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UniProt ID: (Human) Q13131, (Rat) P54645, (Mouse) Q5EG47

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Entrez Gene ID: (Human) 5562, (Rat) 65248, (Mouse) 105787

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Function(s)

chromatin binding protein kinase activity AMP-activated protein kinase activity cAMP-dependent protein kinase activity protein binding ATP binding protein C-terminus binding kinase binding histone serine kinase activity metal ion binding [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase activity tau-protein kinase activity [acetyl-CoA carboxylase] kinase activity protein serine/threonine kinase activity eukaryotic elongation factor-2 kinase activator activity nucleotide binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups non-receptor serine/threonine protein kinase

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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AMPK alpha-1 Monoclonal Antibody (2B7)

AMPK alpha-1 Antibody (MA5-15815) in ICC/IF

Product Details

MA5-15815

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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