Proteintech

AMPK alpha Monoclonal Antibody (1H8E1)

AMPK alpha Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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AMPK alpha Antibody (66536-1-IG) in IHC (P)

Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using 66536-1-Ig (AMPK alpha antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

AMPK alpha Antibody in Western Blot (WB)

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Product Details

66536-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 51 publications 51 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Flow Cytometry (Flow)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Not Applicable

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1H8E1

Immunogen

AMPK alpha Fusion Protein Ag24744 (1-207 aa encoded by BC012622)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MATAEKQKH DGRVKIGHYI LGDTLGVGTF GKVKVGKHEL TGHKVAVKIL NRQKIRSLDV VGKIRREIQN LKLFRHPHII KLYQVISTPS DIFMVMEYVS GGELFDYICK NGRLDEKESR RLFQQILSGV DYCHRHMVVH RDLKPENVLL DAHMNAKIAD FGLSNMMSDG EFLRTSCGSP NYAAPEVISG RDCNIIRILT SQFTNYQH (1-207 aa encoded by BC012622)

Target Information

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 5'-AMP-activated protein kinase catalytic subunit alpha-1; 5'-AMP-activated protein kinase, catalytic alpha-1 chain; AAPK2; ACACA kinase; Acetyl-CoA carboxylase kinase; activated protein kinase; AMP -activate kinase alpha 1 subunit; AMP activated protein kinase; AMP-activated protein kinase, alpha 1 catalytic subunit; AMP-activated protein kinase, catalytic, alpha-1; AMPK alpha 1; AMPK alpha-1 chain; AMPK subunit alpha-1; AMPK subunit pAMPK; AMPK-alpha-2 chain; AMPK2; HMGCR kinase; Hydroxymethylglutaryl-CoA reductase kinase; MGC33776; MGC57364; pAMPK; PRKAA; PRKAA2; protein kinase, AMP-activated, alpha 1 catalytic subunit; Tau-protein kinase PRKAA1

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Gene Aliases: AI194361; AI450832; AL024255; AMPK; AMPK1; AMPKa1; AMPKalpha1; C130083N04Rik; PRKAA1

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UniProt ID: (Human) Q13131, (Rat) P54645, (Mouse) Q5EG47

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Entrez Gene ID: (Human) 5562, (Rat) 65248, (Mouse) 105787

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Function(s)

chromatin binding protein kinase activity AMP-activated protein kinase activity cAMP-dependent protein kinase activity protein binding ATP binding protein C-terminus binding kinase binding histone serine kinase activity metal ion binding [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase activity tau-protein kinase activity [acetyl-CoA carboxylase] kinase activity protein serine/threonine kinase activity eukaryotic elongation factor-2 kinase activator activity nucleotide binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups non-receptor serine/threonine protein kinase

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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AMPK alpha Monoclonal Antibody (1H8E1)

AMPK alpha Antibody (66536-1-IG) in IHC (P)

Product Details

66536-1-IG

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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