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Product Details
CF806213
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI3H7
Immunogen
Human recombinant protein fragment corresponding to amino acids 19-496 of human ANGPT2 produced in E.coli.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
ANG-2 binds to the endothelial cell specific receptor Tie-2, but, in contrast to ANG-1, does not induce tyrosine phosphorylation. Consequently, ANG-2 modulates ANG-1 activation of Tie-2 and, depending on the physiological and biochemical environment, can act either as an agonist or antagonist of Tie-2 induced angiogenesis. The signaling interactions of ANG-1, ANG-2 and Tie-2, along with less characterized ANG-3 and ANG-4, are required for embryonic and adult angiogenesis. Physiologically, ANG-1 and ANG-2 are associated with sprouting, tube formation, and structural integrity of newly formed blood vessels. Mature human ANG-2 is a secreted protein containing 480 amino acid residues. ANG-2 is composed of an alpha-helix-rich coiled coil N-terminal domain and fibrinogen-like C-terminal domain. ANG-2 exists predominantly in the form of a disulfide-linked dimer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ANG-2; Angiopoietin-2; angiopoietin-2a; angiopoietin-2B; Tie2-ligand
Gene Aliases: AGPT2; ANG2; ANGPT2
UniProt ID: (Human) O15123
Entrez Gene ID: (Human) 285
angiogenesis
response to hypoxia
signal transduction
germ cell development
response to radiation
response to mechanical stimulus
response to glucose
negative regulation of cell-substrate adhesion
response to organic cyclic compound
response to activity
negative regulation of angiogenesis
organ regeneration
negative regulation of blood vessel endothelial cell migration
positive regulation of angiogenesis
Tie signaling pathway
leukocyte migration
negative regulation of positive chemotaxis
maternal process involved in female pregnancy
cellular response to growth factor stimulus
glomerulus vasculature development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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