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ANGPT2 Antibody (TA806426) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ANGPT2 (RC217216, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-ANGPT. Positive lysates LY400459 (100 µg) and LC400459 (20 µg) can be purchased separately from OriGene.
Product Details
TA806426
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI4A3
Immunogen
Human recombinant protein fragment corresponding to amino acids 19-496 of human ANGPT2 produced in E.coli.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 1% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
ANG-2 binds to the endothelial cell specific receptor Tie-2, but, in contrast to ANG-1, does not induce tyrosine phosphorylation. Consequently, ANG-2 modulates ANG-1 activation of Tie-2 and, depending on the physiological and biochemical environment, can act either as an agonist or antagonist of Tie-2 induced angiogenesis. The signaling interactions of ANG-1, ANG-2 and Tie-2, along with less characterized ANG-3 and ANG-4, are required for embryonic and adult angiogenesis. Physiologically, ANG-1 and ANG-2 are associated with sprouting, tube formation, and structural integrity of newly formed blood vessels. Mature human ANG-2 is a secreted protein containing 480 amino acid residues. ANG-2 is composed of an alpha-helix-rich coiled coil N-terminal domain and fibrinogen-like C-terminal domain. ANG-2 exists predominantly in the form of a disulfide-linked dimer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ANG-2; Angiopoietin-2; angiopoietin-2a; angiopoietin-2B; Tie2-ligand
Gene Aliases: AGPT2; ANG2; ANGPT2
UniProt ID: (Human) O15123
Entrez Gene ID: (Human) 285
angiogenesis
response to hypoxia
signal transduction
germ cell development
response to radiation
response to mechanical stimulus
response to glucose
negative regulation of cell-substrate adhesion
response to organic cyclic compound
response to activity
negative regulation of angiogenesis
organ regeneration
negative regulation of blood vessel endothelial cell migration
positive regulation of angiogenesis
Tie signaling pathway
leukocyte migration
negative regulation of positive chemotaxis
maternal process involved in female pregnancy
cellular response to growth factor stimulus
glomerulus vasculature development
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