Invitrogen

ATP1A1 Monoclonal Antibody (M7-PB-E9)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

6 Published Figures

33 References

View all (40) ATP1A1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

ATP1A1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 18

ATP1A1 Antibody (MA3-928) in ICC/IF

Immunofluorescent analysis of Sodiµm/Potassium ATPase alpha using Sodiµm/Potassium ATPase alpha Monoclonal Antibody (M7-PB-E9) (Product # MA3-928) shows staining in MCF-7 Cells. Sodiµm/Potassium ATPase alpha (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Sodiµm/Potassium ATPase alpha (Product # MA3-928) at a dilution of 1:20 over night at 4 °C, washed wi... View More

ATP1A1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ATP1A1 Antibody in Immunohistochemistry (IHC)

ATP1A1 Antibody in Western Blot, Immunoprecipitation (WB, IP)

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Product Details

MA3-928

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:5,000

View 16 publications 16 publications

Immunohistochemistry (IHC)

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

Assay-dependent

View 4 publications 4 publications

Flow Cytometry (Flow)

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

Immunomicroscopy (IM)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Dog, Chicken, Human, Mouse, Sheep, Pig, Rat

Published species

Dog, Human, Mouse, Pig, Rabbit, Rat, Sheep

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

M7-PB-E9

Immunogen

Purified alpha sodium/potassium ATPase from sheep kidney.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.87 mg/mL

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2060976

Product Specific Information

MA3-928 detects sodium/potassium ATPase alpha from human, mouse, bovine, sheep, canine, chicken and porcine tissues. This antibody detects the alpha 3 isoform in rat only.

MA3-928 has been successfully used in Western blot, immunohistochemistry, immunoprecipitation, immunocytochemistry, immunofluorescence, and ELISA procedures. By Western blot, this antibody detects an ~110 kDa protein representing alpha sodium/potassium ATPase from MDCK cell extract. Immunohistochemical staining of alpha sodium/potassium ATPase in porcine heart with MA3-928 yields a pattern consistent with the plasma membrane localization.

The MA3-928 antigen is purified sheep kidney alpha sodium/potassium ATPase. This antibody recognizes an epitope between amino acid residues 646 and 652 of the sheep kidney alpha sodium/potassium ATPase.

Target Information

The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Deletion of Emc1 in photoreceptor cells causes retinal degeneration in mice.

The FEBS journal

Li X,Jiang Z,Su Y,Wang K,Jiang X,Sun K,Yang Y,Zhou Y,Zhu X,Zhang L

MA3-928 was used in Immunohistochemistry-immunofluorescence to demonstrate the essential roles of Emc1 in photoreceptor cells, and reveals the mechanism through which EMC1 mutations are linked to retinitis pigmentosa.

Fri Sep 01 00:00:00 EDT 2023

Norepinephrine activates β₁ -adrenergic receptors at the inner nuclear membrane in astrocytes.

Glia

Benton KC,Wheeler DS,Kurtoglu B,Ansari MBZ,Cibich DP,Gonzalez DA,Herbst MR,Khursheed S,Knorr RC,Lobner D,Maglasang JG,Rohr KE,Taylor A,Twining RC,Witt PJ,Gasser PJ

MA3-928 was used in Western Blotting to reveal a novel mechanism by which norepinephrine may directly influence nuclear processes.

Thu Sep 01 00:00:00 EDT 2022

Targeting cholesteryl ester accumulation in the heart improves cardiac insulin response.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Actis Dato V,Benitez-Amaro A,Garcia E,Claudi L,Lhoëst MTL,Iborra A,Escola-Gil JC,Guerra JM,Samouillan V,Enrich C,Chiabrando G,Llorente-Cortés V

MA3-928 was used in Western Blot to evaluate the effects of P3 peptide immunization on the alterations induced by a high-fat diet (HFD) in cardiac insulin response.

Mon Aug 01 00:00:00 EDT 2022

G_i/o protein-coupled receptor inhibition of beta-cell electrical excitability and insulin secretion depends on Na⁺/K⁺ ATPase activation.

Nature communications

Dickerson MT,Dadi PK,Zaborska KE,Nakhe AY,Schaub CM,Dobson JR,Wright NM,Lynch JC,Scott CF,Robinson LD,Jacobson DA

MA3-928 was used in Immunohistochemistry (Paraffin) to reveal that NKA-mediated β-cell membrane potential hyperpolarization is the primary and conserved mechanism for Gi/o-GPCR control of electrical excitability, Ca2+ handling, and insulin secretion.

Sat Oct 29 00:00:00 EDT 2022

Deletion of Asrgl1 Leads to Photoreceptor Degeneration in Mice.

Frontiers in cell and developmental biology

Zhou Y,Tian W,Jiang X,Yang H,Jiang Z,Li X,Jiang D,Sun K,Yang Y,Liu W,Zhu X

MA3-928 was used in Immunohistochemistry to provide a knockout mouse model for a better understanding of the molecular mechanism for ASRGL1-related RP.

Sat Feb 05 00:00:00 EST 2022

Deletion of the Impg2 gene causes the degeneration of rod and cone cells in mice.

Human molecular genetics

Xu H,Qu C,Gan L,Sun K,Tan J,Liu X,Jiang Z,Tian W,Liu W,Zhang S,Yang Y,Jiang L,Zhu X,Zhang L

MA3-928 was used in Immunohistochemistry to develop two independent Impg2 knockout (KO) mouse models using the CRISPR/Cas9 technique to assess the in vivo functions of Impg2 in the retina.

Sat Jun 27 00:00:00 EDT 2020

Novel monoclonal antibodies against thymidine kinase 1 and their potential use for the immunotargeting of lung, breast and colon cancer cells.

Cancer cell international

Velazquez EJ,Brindley TD,Shrestha G,Bitter EE,Cress JD,Townsend MH,Berges BK,Robison RA,Weber KS,O'Neill KL

MA3-928 was used in Western Blotting to show potential to be used to detect and target TK1 on the membrane of various tumor cells.

Mon Nov 13 00:00:00 EST 2023

Novel monoclonal antibodies against thymidine kinase 1 and their potential use for the immunotargeting of lung, breast and colon cancer cells.

Cancer cell international

Velazquez EJ,Brindley TD,Shrestha G,Bitter EE,Cress JD,Townsend MH,Berges BK,Robison RA,Weber KS,O'Neill KL

MA3-928 was used in Western Blotting to show potential to be used to detect and target TK1 on the membrane of various tumor cells.

Mon Nov 13 00:00:00 EST 2023

Astrocyte deletion of α2-Na/K ATPase triggers episodic motor paralysis in mice via a metabolic pathway.

Nature communications

Smith SE,Chen X,Brier LM,Bumstead JR,Rensing NR,Ringel AE,Shin H,Oldenborg A,Crowley JR,Bice AR,Dikranian K,Ippolito JE,Haigis MC,Papouin T,Zhao G,Wong M,Culver JP,Bonni A

MA3-928 was used in Western Blotting to show episodic paralysis can be induced in mice with ion pump α2-Na/K ATPase deletion.

Wed Dec 02 00:00:00 EST 2020

GPR35 promotes glycolysis, proliferation, and oncogenic signaling by engaging with the sodium potassium pump.

Science signaling

Schneditz G,Elias JE,Pagano E,Zaeem Cader M,Saveljeva S,Long K,Mukhopadhyay S,Arasteh M,Lawley TD,Dougan G,Bassett A,Karlsen TH,Kaser A,Kaneider NC

Published figure using ATP1A1 monoclonal antibody (Product # MA3-928) in Western Blot

Tue Jan 01 00:00:00 EST 2019

GPR35 promotes glycolysis, proliferation, and oncogenic signaling by engaging with the sodium potassium pump.

Science signaling

Schneditz G,Elias JE,Pagano E,Zaeem Cader M,Saveljeva S,Long K,Mukhopadhyay S,Arasteh M,Lawley TD,Dougan G,Bassett A,Karlsen TH,Kaser A,Kaneider NC

Published figure using ATP1A1 monoclonal antibody (Product # MA3-928) in Western Blot

Tue Jan 01 00:00:00 EST 2019

Whole-exome sequencing revealed HKDC1 as a candidate gene associated with autosomal-recessive retinitis pigmentosa.

Human molecular genetics

Zhang L,Sun Z,Zhao P,Huang L,Xu M,Yang Y,Chen X,Lu F,Zhang X,Wang H,Zhang S,Liu W,Jiang Z,Ma S,Chen R,Zhao C,Yang Z,Sui R,Zhu X

MA3-928 was used in Immunohistochemistry-immunofluorescence to demonstrate how HKDC1 is associated with autosomal recessively inherited retinitis pigmentosa.

Sat Dec 01 00:00:00 EST 2018

Targeting prohibitins with chemical ligands inhibits KRAS-mediated lung tumours.

Oncogene

Yurugi H,Marini F,Weber C,David K,Zhao Q,Binder H,Désaubry L,Rajalingam K

MA3928 was used in western blot to show that PHB1 is highly expressed in non-small cell lung cancers patients and correlates with poor survival

Thu Aug 17 00:00:00 EDT 2017

The subcellular localization of GABA transporters and its implication for seizure management.

Neurochemical research

Madsen KK,Hansen GH,Danielsen EM,Schousboe A

MA3-928 was used in western blot to study seizure management by subcellular localization of GABA transporters

Sun Feb 01 00:00:00 EST 2015

Flotillin-2 expression in the human gut: from a cell model to human tissue in health and inflammatory bowel diseases.

International journal of medical sciences

Gauss A,Buchholz I,Zahn A,Schmitz G,Stremmel W,Fuellekrug J,Ehehalt R

MA3-928 was used in immunocytochemistry to study of Flotillin-2 expression from a cell model to human tissue in health and inflammatory bowel disease

Mon Mar 17 00:00:00 EDT 2014

Flotillin-2 expression in the human gut: from a cell model to human tissue in health and inflammatory bowel diseases.

International journal of medical sciences

Gauss A,Buchholz I,Zahn A,Schmitz G,Stremmel W,Fuellekrug J,Ehehalt R

MA3-928 was used in immunocytochemistry to study of Flotillin-2 expression from a cell model to human tissue in health and inflammatory bowel disease

Mon Mar 17 00:00:00 EDT 2014

Targeted entry via somatostatin receptors using a novel modified retrovirus glycoprotein that delivers genes at levels comparable to those of wild-type viral glycoproteins.

Journal of virology

Li F,Ryu BY,Krueger RL,Heldt SA,Albritton LM

MA3-928 was used in western blot to evaluate a new gene transfection strategy based on a modified retrovirus glycoprotein

Sun Jan 01 00:00:00 EST 2012

Modulation of vH+-ATPase is part of the functional adaptation of sheep rumen epithelium to high-energy diet.

American journal of physiology. Regulatory, integrative and comparative physiology

Kuzinski J,Zitnan R,Albrecht E,Viergutz T,Schweigel-Röntgen M

MA3-928 was used in flow cytometry to study the role of the rumenal vacuolar H(+)-ATPase in the response of ovine rumen epithelial cells to an energy-rich dietary regimen

Thu Nov 01 00:00:00 EDT 2012

POST, partner of stromal interaction molecule 1 (STIM1), targets STIM1 to multiple transporters.

Proceedings of the National Academy of Sciences of the United States of America

Krapivinsky G,Krapivinsky L,Stotz SC,Manasian Y,Clapham DE

MA3-928 was used in western blot to investigate the function of POST in STIM1 localization

Tue Nov 29 00:00:00 EST 2011

Early uptake and continuous accumulation of thallium-201 chloride in a benign mixed tumor of soft tissue: case report.

Diagnostic pathology

Nagata S,Jin YF,Yoshizato K,Kitamura M,Iizuka N,Song M,Tomoeda M,Yuki M,Kubo C,Yoshizawa H,Outani H,Hamada K,Araki N,Funauchi M,Tomita Y

MA3-928 was used in immunohistochemistry to report a clinical case of benign mixed tumor of soft tissu with unique Tl-201 scintigraphic features

Sun May 30 00:00:00 EDT 2010

Contraceptive steroids from pharmaceutical waste perturbate junctional communication in Sertoli cells.

Biochimie

Tramoni M,Gilleron J,Tahiri K,Carette D,Corvol MT,Segretain D,Pointis G,Savouret JF

MA3-928 was used in immunohistochemistry to study the effect of contraceptive steroids on gap junction activity in Sertoli cells

Fri Apr 23 00:00:00 EDT 2010

Ion transport and barrier function in a telomerase-immortalized human nondysplastic, Barrett's cell line (BAR-T).

Diseases of the esophagus : official journal of the International Society for Diseases of the Esophagus

Jovov B,Orlando GS,Tobey NA,Brown KL,Djukic Z,Carson JL,Brighton LE,Orlando RC

MA3-928 was used in immunocytochemistry to evaluate an immortalized human nondysplastic Barrett cell line for the study of epithelial functions

Thu Oct 29 00:00:00 EDT 2009

Leptin and the obesity receptor (OB-R) in the small intestine and colon: a colocalization study.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

Hansen GH,Niels-Christiansen LL,Danielsen EM

MA3-928 was used in immunohistochemistry to study the localization of the leptin and the obesity receptor in the small intestine and colon.

Tue Jul 01 00:00:00 EDT 2008

Lateral membrane biogenesis in human bronchial epithelial cells requires 190-kDa ankyrin-G.

The Journal of biological chemistry

Kizhatil K,Bennett V

MA3-928 was used in immunocytochemistry to investigate the role of 190-kDa ankyrin-G in the assembly of lateral membranes of bronchial epithelial cells.

Fri Apr 16 00:00:00 EDT 2004

Presenilins and gamma-secretase inhibitors affect intracellular trafficking and cell surface localization of the gamma-secretase complex components.

The Journal of biological chemistry

Wang H,Luo WJ,Zhang YW,Li YM,Thinakaran G,Greengard P,Xu H

MA3-928 was used in western blot to study the role of presenilins in intracellular trafficking of the gamma-secretase components.

Fri Sep 24 00:00:00 EDT 2004

Identification of a critical basic residue on the ecotropic murine leukemia virus receptor.

Journal of virology

Qian Z,Donald R,Wang H,Chen Q,Albritton LM

MA3-928 was used in western blot to identify the critical residues in ecotropic murine leukemia virus receptor influencing MLV binding and infection.

Fri Aug 01 00:00:00 EDT 2003

Coronin is involved in uptake of Mycobacterium bovis BCG in human macrophages but not in phagosome maintenance.

Cellular microbiology

Schüller S,Neefjes J,Ottenhoff T,Thole J,Young D

MA3-928 was used in western blot to study the role of coronin in Mycobacterium bovis BCG infection

Sat Dec 01 00:00:00 EST 2001

Plasma membrane depolarization without repolarization is an early molecular event in anti-Fas-induced apoptosis.

The Journal of biological chemistry

Bortner CD,Gomez-Angelats M,Cidlowski JA

MA3-928 was used in western blot to investigate the potential movement of monovalent ions in Jurkat cells in the early stage of anti-Fas-induced apoptosis.

Fri Feb 09 00:00:00 EST 2001

Loss of cyclosporin and azidopine binding are associated with altered ATPase activity by a mutant P-glycoprotein with deleted phe(335).

Molecular pharmacology

Chen KG,Lacayo NJ,Durán GE,Cohen D,Sikic BI

MA3-928 was used in western blot to investigate the phenotype caused by a P-glycoprotein with a deletion mutation of Phe335 and the possible roles of Phe335 in forming the major binding sites for cyclosporins and azidopine

Sat Apr 01 00:00:00 EST 2000

Intracellular redirection of plasma membrane trafficking after loss of epithelial cell polarity.

Molecular biology of the cell

Low SH,Miura M,Roche PA,Valdez AC,Mostov KE,Weimbs T

MA3-928 was used in immunocytochemistry to study the intracellular redirection of plasma membrane trafficking after loss of epithelial cell polarity.

Fri Sep 01 00:00:00 EDT 2000

Glut-1 and hexokinase expression: relationship with 2-fluoro-2-deoxy-D-glucose uptake in A431 and T47D cells in culture.

Cancer research

Aloj L,Caracó C,Jagoda E,Eckelman WC,Neumann RD

MA3-928 was used in western blot to study the glut-1 and hexokinase expression in six different cancer cell lines

Wed Sep 15 00:00:00 EDT 1999

Nitric oxide synthase in cardiac sarcoplasmic reticulum.

Proceedings of the National Academy of Sciences of the United States of America

Xu KY,Huso DL,Dawson TM,Bredt DS,Becker LC

Tue Jan 19 00:00:00 EST 1999

Nitric oxide synthase in cardiac sarcoplasmic reticulum.

Proceedings of the National Academy of Sciences of the United States of America

Xu KY,Huso DL,Dawson TM,Bredt DS,Becker LC

Tue Jan 19 00:00:00 EST 1999

Identification of the amino acids comprising a surface-exposed epitope within the nucleotide-binding domain of the Na+,K(+)-ATPase using a random peptide library.

Protein science : a publication of the Protein Society

Malik B,Jamieson GA,Ball WJ

MA3-928 was used in western blot to investigate the amino acid sequence of the surface-exposed epitope in sodium/potassium-ATPase's nucleotide-binding domain

Wed Dec 01 00:00:00 EST 1993

Bioinformatics

Protein Aliases: (Na+ + K+)-ATPase; (Na+, K+)-ATPase alpha-subunit; ATP1B; ATPase; ATPase, Na+/K+ transporting, alpha 1 polypeptide; ATPase, Na+K+ transporting, alpha 1; MGC1798; Na(+)/K(+) ATPase alpha-1 subunit; Na+ ,K+ -ATPase alpha subunit; Na+ /K+ ATPase alpha 1 subunit; Na+, K+-ATPase beta-subunit precursor; Na+/K+ ATPase 1; Na+/K+ ATPase Alpha; Na+/K+ transporting alpha 1 polypeptide; Na, K-ATPase, alpha-A catalytic polypeptide; Na,K-ATPase alpha-1 subunit; Na,K-ATPase catalytic subunit alpha-A protein; RP4-655J12.1; Sodium Potassium ATPase; sodium pump 1; Sodium pump subunit alpha-1; sodium-potassium ATPase catalytic subunit alpha-1; sodium-potassium-ATPase, alpha 1 polypeptide; sodium/potassium-transporting ATPase alpha-1 chain; Sodium/potassium-transporting ATPase subunit alpha-1; unnamed protein product

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Gene Aliases: ATP1A1; Atpa-1; BC010319; Nkaa1b

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UniProt ID: (Sheep) P04074, (Human) P05023, (Pig) P05024, (Bovine) Q08DA1, (Chicken) P09572, (Rat) P06685, (Mouse) Q8VDN2

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Entrez Gene ID: (Dog) 403992, (Sheep) 443381, (Human) 476, (Pig) 397481, (Bovine) 282144, (Chicken) 396530, (Rat) 24211, (Mouse) 11928

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Function(s)

sodium:potassium-exchanging ATPase activity ATP binding metal ion binding protein binding phosphatase activity protein kinase binding protein domain specific binding ankyrin binding potassium ion binding sodium ion binding ADP binding phosphatidylinositol 3-kinase binding chaperone binding steroid hormone binding ATPase binding nucleotide binding hydrolase activity primary active transporter

Process(es)

regulation of sodium ion transport sodium ion transport establishment or maintenance of transmembrane electrochemical gradient potassium ion transmembrane transport regulation of the force of heart contraction cellular sodium ion homeostasis regulation of blood pressure potassium ion import ATP hydrolysis coupled proton transport dephosphorylation cellular potassium ion homeostasis negative regulation of glucocorticoid biosynthetic process ion transmembrane transport sodium ion export from cell response to drug negative regulation of heart contraction positive regulation of heart contraction positive regulation of striated muscle contraction relaxation of cardiac muscle cardiac muscle contraction membrane hyperpolarization cellular response to mechanical stimulus cellular response to steroid hormone stimulus regulation of cardiac muscle cell contraction membrane repolarization membrane repolarization during cardiac muscle cell action potential cell communication by electrical coupling involved in cardiac conduction response to glycoside regulation of cardiac conduction potassium ion import across plasma membrane transmembrane transport potassium ion transport energy coupled proton transmembrane transport, against electrochemical gradient sodium ion transmembrane transport transport ion transport

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ATP1A1 Monoclonal Antibody (M7-PB-E9)

ATP1A1 Antibody (MA3-928) in ICC/IF

Product Details

MA3-928

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

Immunomicroscopy (IM)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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