Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 2
Alpha A Crystallin Antibody (PA5-120942) in IHC (P)
Immunohistochemistry (Immunofluorescence) analysis of Alpha A Crystallin in rat eye. Samples were incubated with Alpha A Crystallin Polyclonal antibody (Product # PA5-120942) using a dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
Product Details
PA5-120942
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat,
Zebrafish
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-173 of human CRYAA (NP_000385.1).
Conjugate
Form
Liquid
Concentration
1.07 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2914514
Product Specific Information
Positive test controls include: Mouse kidney, Mouse liver, Mouse heart. The target is usually found in the following locations: Cytoplasm, Nucleus.
Immunogen sequence: MDVTIQHPWF KRTLGPFYPS RLFDQFFGEG LFEYDLLPFL SSTISPYYRQ SLFRTVLDSG ISEVRSDRDK FVIFLDVKHF SPEDLTVKVQ DDFVEIHGKH NERQDDHGYI SREFHRRYRL PSNVDQSALS CSLSADGMLT FCGPKIQTGL DATHAERAIP VSREEKPTSA PSS
Target Information
Lens proteins consist almost entirely of crystallins (about 95%). Crystallins are also found vertebrate skeletal muscle tissue. In the lens, their structural function is to assist in maintaining the proper refractive index of the lens. The mammalian lens contains 3 major classes of crystallins: alpha, beta, and gamma. Alpha-crystallin is the largest of the crystallins and is composed of 2 primary gene products-alpha-A and alpha-B. There are at least 5 different proteins comprising the beta-crystallins. The gamma-crystallins are monomeric, but there are at least 5 gamma crystallins identified in bovine and rat lens. Alpha-Crystallin comprises 40% of total lens protein composition. In addition to maintaining proper refractive index, it also functions in a chaperone like manner by preventing the formation of aggregates possibly leading to cataract formation. It is believed that the phosphorylated states of the alpha-crystallin occur in response to cellular stress and may serve a structural control function and play a role in protein maintenance. Alpha-B crystallin has been linked to Alexander's disease where it accumulates in brain cells of those afflicted.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: [a]A-crystallin; Acry 1; alpha crystallin A chain; Alpha-crystallin A chain; CRYA 1; Crystallin Alpha 1; crystallin, alpha 1; crystallin, alpha A; Crystallin, alpha polypeptide A; crystallin, alpha-1; Heat shock protein beta-4; Heat shock protein family B member 4; HSPB 4; HspB4; human alphaA-crystallin (CRYA1); lens opacity 18; small heat shock protein
Gene Aliases: Acry-1; Crya-1; CRYA1; CRYAA; CTRCT9; DAcry-1; HSPB4; lop18; wu:fc06h04; zgc:92036
UniProt ID: (Human) P02489, (Rat) P24623, (Mouse) P24622
Entrez Gene ID: (Human) 1409, (Rat) 24273, (Mouse) 12954, (Human) 102724652, (Zebrafish) 100000769
response to hypoxia
positive regulation of protein phosphorylation
lens development in camera-type eye
lens morphogenesis in camera-type eye
protein folding
apoptotic process
mitochondrion organization
actin filament organization
microtubule-based process
tubulin complex assembly
visual perception
response to lead ion
negative regulation of gene expression
positive regulation of cell growth
negative regulation of intracellular transport
response to drug
response to hydrogen peroxide
camera-type eye development
negative regulation of apoptotic process
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
embryonic camera-type eye morphogenesis
protein homooligomerization
response to glucocorticoid
apoptotic process involved in morphogenesis
response to UV-A
lens fiber cell morphogenesis
protein refolding
response to stimulus
eye development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support