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Alpha E catenin Polyclonal Antibody

Alpha E catenin Antibody in Immunocytochemistry (ICC/IF)

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Alpha E catenin Antibody (12831-1-AP) in ICC/IF

Immunofluorescent analysis of A431 cells using 12831-1-AP (Alpha E catenin antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IGG.

Alpha E catenin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Alpha E catenin Antibody in Western Blot (WB)

Alpha E catenin Antibody in Flow Cytometry (Flow)

Alpha E catenin Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

12831-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 11 publications 11 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

View 2 publications 2 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Not applicable (N/A)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Alpha E catenin Fusion Protein Ag3565 (188-536 aa encoded by BC031262)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.41 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: SEMDNYEPG VYTEKVLEAT KLLSNTVMPR FTEQVEAAVE ALSSDPAQPM DENEFIDASR LVYDGIRDIR KAVLMIRTPE ELDDSDFETE DFDVRSRTSV QTEDDQLIAG QSARAIMAQL PQEQKAKIAE QVASFQEEKS KLDAEVSKWD DSGNDIIVLA KQMCMIMMEM TDFTRGKGPL KNTSDVISAA KKIAEAGSRM DKLGRTIADH CPDSACKQDL LAYLQRIALY CHQLNICSKV KAEVQNLGGE LVVSGVDSAM SLIQAAKNLM NAVVQTVKAS YVASTKYQKS QGMASLNLPA VSWKMKAPEK KPLVKREKQD ETQTKIKRAS QKKHVNPVQA LSEFKAMDSI (188-536 aa encoded by BC031262)

Target Information

Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherence junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Scientific reports

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Wed Jun 17 00:00:00 EDT 2020

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Experimental and therapeutic medicine

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GATA4 is upregulated in nasopharyngeal cancer and facilitates epithelial-mesenchymal transition and metastasis through regulation of SLUG.

Experimental and therapeutic medicine

Zhou Y,Chang H,Yang B

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Transducin (β)-like 1 X-linked receptor 1 promotes gastric cancer progression via the ERK1/2 pathway.

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Cancer science

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Wnt signaling regulation of stem-like properties in human lung adenocarcinoma cell lines.

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Wnt signaling regulation of stem-like properties in human lung adenocarcinoma cell lines.

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Helicobacter pylori dwelling on the apical surface of gastrointestinal epithelium damages the mucosal barrier through direct contact.

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Bioinformatics

Protein Aliases: 102 kDa cadherin-associated protein; alpha E catenin; Alpha E-catenin; alpha(E)-catenin; alpha-E-catenin; cadherin associated protein; Cadherin-associated protein; CAP102; catenin (cadherin associated protein), alpha 1; catenin (cadherin-associated protein), alpha 1, 102kDa; catenin alpha 1; Catenin alpha-1; catenin, alpha 1; Renal carcinoma antigen NY-REN-13

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Gene Aliases: 2010010M04Rik; AA517462; AI988031; CAP102; Catna1; CTNNA1; MDPT2

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UniProt ID: (Human) P35221, (Mouse) P26231

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Entrez Gene ID: (Human) 1495, (Rat) 307505, (Mouse) 12385

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Function(s)

structural molecule activity protein binding beta-catenin binding vinculin binding poly(A) RNA binding gamma-catenin binding cadherin binding actin filament binding cadherin binding involved in cell-cell adhesion non-motor actin binding protein

Process(es)

ovarian follicle development actin filament organization cell adhesion establishment or maintenance of cell polarity negative regulation of neuroblast proliferation aging male gonad development gap junction assembly axon regeneration adherens junction organization cellular protein localization odontogenesis of dentin-containing tooth apical junction assembly response to estrogen positive regulation of smoothened signaling pathway positive regulation of muscle cell differentiation protein heterooligomerization cellular response to indole-3-methanol epithelial cell-cell adhesion negative regulation of cell motility negative regulation of integrin-mediated signaling pathway negative regulation of extrinsic apoptotic signaling pathway in absence of ligand positive regulation of extrinsic apoptotic signaling pathway in absence of ligand response to organic cyclic compound regulation of cell proliferation negative regulation of apoptotic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Alpha E catenin Polyclonal Antibody

Alpha E catenin Antibody (12831-1-AP) in ICC/IF

Product Details

12831-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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