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FIGURE: 1 / 3
Angiopoietin 2 Antibody (100-401-402) in ICC/IF
Immunocytochemistry of anti-ANG2 Antibody in PC12 cells. Tissue: Human prostate cancer cell line (PC12). Fixation: 5 min in 100% methanol. Antigen Retrieval/permeabilization: 1hr 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween. Primary antibody: 1:200 dilution overnight at +4°C . Secondary Antibody: Gt anti-rabbit 488 at 1:1000 for 1 hr RT. Staining: Ang2 stained yellow, plasma membranes sta... View More
Product Details
100-401-402
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-Angiopoietin 2 Antiserum was prepared by repeated immunizations with a synthetic peptide, corresponding to a region near the N-terminus of mouse angiopoietin-2 protein, conjugated to KLH using maleimide.
Conjugate
Form
Liquid
Concentration
80 mg/mL
Storage buffer
0.02M potassium phosphate/whole serum, pH 7.2, with 0.15M NaCl
Contains
0.1% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Angiopoietin 2 Antibody antiserum is directed against mouse angiopoietin-2 and shows no reactivity with angiopoietin-1 from mouse-derived proteins. This reagent cross-reacts with human angiopoietin-2. Partially cross-reactivity is noted with human angiopoietin-1.
Target Information
ANG-2 binds to the endothelial cell specific receptor Tie-2, but, in contrast to ANG-1, does not induce tyrosine phosphorylation. Consequently, ANG-2 modulates ANG-1 activation of Tie-2 and, depending on the physiological and biochemical environment, can act either as an agonist or antagonist of Tie-2 induced angiogenesis. The signaling interactions of ANG-1, ANG-2 and Tie-2, along with less characterized ANG-3 and ANG-4, are required for embryonic and adult angiogenesis. Physiologically, ANG-1 and ANG-2 are associated with sprouting, tube formation, and structural integrity of newly formed blood vessels. Mature human ANG-2 is a secreted protein containing 480 amino acid residues. ANG-2 is composed of an alpha-helix-rich coiled coil N-terminal domain and fibrinogen-like C-terminal domain. ANG-2 exists predominantly in the form of a disulfide-linked dimer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ANG-2; Angiopoietin-2; angiopoietin-2a; angiopoietin-2B; Tie2-ligand
Gene Aliases: AGPT2; Ang-2; ANG2; ANGPT2
UniProt ID: (Human) O15123, (Mouse) O35608
Entrez Gene ID: (Human) 285, (Mouse) 11601
angiogenesis
response to hypoxia
signal transduction
germ cell development
response to radiation
response to mechanical stimulus
response to glucose
negative regulation of cell-substrate adhesion
response to organic cyclic compound
response to activity
negative regulation of angiogenesis
organ regeneration
negative regulation of blood vessel endothelial cell migration
positive regulation of angiogenesis
Tie signaling pathway
leukocyte migration
negative regulation of positive chemotaxis
maternal process involved in female pregnancy
cellular response to growth factor stimulus
glomerulus vasculature development
blood vessel remodeling
transmembrane receptor protein tyrosine kinase signaling pathway
multicellular organism development
endoderm development
hemopoiesis
cell differentiation
regulation of angiogenesis
blood vessel morphogenesis
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