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Angiopoietin 2 Antibody (711186) in WB
Western blot analysis was performed on tissue extracts (30 µg lysate) of Mouse Brain (Lane 1) and Mouse Liver (Lane 2). The blots were probed with Anti- ANGPT2 Recombinant Rabbit Polyclonal Antibody (Product # 711186, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 57 kDa band corresponding to ANGPT2 was observed specifically in the relevant liver tissue and not in other tissues tested. Known quantity of pr... View More
Product Details
711186
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Immunogen
Protein corresponding to Human ANGPT2 (aa 250-496)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2633130
Product Specific Information
This antibody is predicted to react with Monkey, Horse, Pig and Rabbit
Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
Target Information
ANG-2 binds to the endothelial cell specific receptor Tie-2, but, in contrast to ANG-1, does not induce tyrosine phosphorylation. Consequently, ANG-2 modulates ANG-1 activation of Tie-2 and, depending on the physiological and biochemical environment, can act either as an agonist or antagonist of Tie-2 induced angiogenesis. The signaling interactions of ANG-1, ANG-2 and Tie-2, along with less characterized ANG-3 and ANG-4, are required for embryonic and adult angiogenesis. Physiologically, ANG-1 and ANG-2 are associated with sprouting, tube formation, and structural integrity of newly formed blood vessels. Mature human ANG-2 is a secreted protein containing 480 amino acid residues. ANG-2 is composed of an alpha-helix-rich coiled coil N-terminal domain and fibrinogen-like C-terminal domain. ANG-2 exists predominantly in the form of a disulfide-linked dimer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ANG-2; Angiopoietin-2; angiopoietin-2a; angiopoietin-2B; Tie2-ligand
Gene Aliases: AGPT2; Ang-2; ANG2; ANGPT2
UniProt ID: (Human) O15123, (Mouse) O35608
Entrez Gene ID: (Human) 285, (Mouse) 11601
angiogenesis
response to hypoxia
signal transduction
germ cell development
response to radiation
response to mechanical stimulus
response to glucose
negative regulation of cell-substrate adhesion
response to organic cyclic compound
response to activity
negative regulation of angiogenesis
organ regeneration
negative regulation of blood vessel endothelial cell migration
positive regulation of angiogenesis
Tie signaling pathway
leukocyte migration
negative regulation of positive chemotaxis
maternal process involved in female pregnancy
cellular response to growth factor stimulus
glomerulus vasculature development
blood vessel remodeling
transmembrane receptor protein tyrosine kinase signaling pathway
multicellular organism development
endoderm development
hemopoiesis
cell differentiation
regulation of angiogenesis
blood vessel morphogenesis
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