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ApoA2 Antibody (MA5-42481) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of of Apolipoprotein A II in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with ApoA2 Recombinant Monoclonal Antibody (Product # MA5-42481) at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nu... View More
Product Details
MA5-42481
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JB70-35
Immunogen
Recombinant full length protein of human Apolipoprotein A II
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2911622
Product Specific Information
Positive Control: HepG2, LOVO, SH-SY5Y, rat large intestine tissue, rat liver tissue, human liver tissue, mouse large intestine tissue.
Subcellular Location: Secreted.
Target Information
At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL, and VLDL particles of human serum are often used to estimate an individual's relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins ( especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease that measurements of lipoprotein particles (especially HDL and LDL). The apolipoproteins are derived from human plasma by density gradient ultracentrifugation and HPLC.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: APO A2; Apo-AII; Apolipoprotein; Apolipoprotein A-II; Apolipoprotein A2; Apolipoprotein AII
Gene Aliases: Alp-2; Apo-AII; Apoa-2; ApoA-II; APOA2; APOAII; Hdl-1
UniProt ID: (Human) P02652, (Rat) P04638, (Mouse) P09813
Entrez Gene ID: (Human) 336, (Rat) 25649, (Mouse) 11807
lipid transporter activity
protein binding
phospholipid binding
high-density lipoprotein particle binding
lipid binding
cholesterol binding
cholesterol transporter activity
phosphatidylcholine binding
apolipoprotein receptor binding
protein homodimerization activity
protein heterodimerization activity
lipase inhibitor activity
phosphatidylcholine-sterol O-acyltransferase activator activity
high-density lipoprotein particle receptor binding
apolipoprotein
retinoid metabolic process
acute inflammatory response
negative regulation of cytokine secretion involved in immune response
triglyceride metabolic process
phosphatidylcholine biosynthetic process
cholesterol metabolic process
phospholipid catabolic process
response to glucose
positive regulation of cholesterol esterification
negative regulation of very-low-density lipoprotein particle remodeling
viral process
protein oxidation
peptidyl-methionine modification
regulation of intestinal cholesterol absorption
organ regeneration
regulation of protein stability
negative regulation of cholesterol transport
cholesterol efflux
phospholipid efflux
triglyceride-rich lipoprotein particle remodeling
low-density lipoprotein particle remodeling
high-density lipoprotein particle remodeling
high-density lipoprotein particle assembly
high-density lipoprotein particle clearance
lipoprotein metabolic process
lipoprotein biosynthetic process
response to drug
cholesterol homeostasis
positive regulation of catalytic activity
response to estrogen
reverse cholesterol transport
positive regulation of interleukin-8 biosynthetic process
diacylglycerol catabolic process
negative regulation of lipid catabolic process
positive regulation of lipid catabolic process
response to glucocorticoid
negative regulation of lipase activity
negative regulation of cholesterol import
negative regulation of cholesterol transporter activity
protein folding
lipid transport
cholesterol transport
fatty acid metabolic process
transport
beta-glucoside transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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