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Invitrogen
Apolipoprotein B Monoclonal Antibody (F2C13)
FIGURE: 1 / 2
Apolipoprotein B Antibody (MA5-14741) in ICC/IF
Immunofluorescence analysis of Apolipoprotein B was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Apolipoprotein B (F2C13) Mouse Monoclonal Antibody (Product # MA5-14741) at 2 µg/mL in 0.1% BSA and incu... View More
Product Details
MA5-14741
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
F2C13
Immunogen
Purified human serum LDL
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Ion-exchange chromatography
Storage buffer
PBS, pH 7.4, with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10985017
Product Specific Information
MA5-14741 targets Apolipoprotein B in ELISA, IP, and RIA applications and shows reactivity with Human samples.
The MA5-14741 immunogen is purified human serum LDL.
MA5-14741 detects Apolipoprotein B which has a predicted molecular weight of approximately 513 kDa.
Product MA514741 is a smaller package size of MIA1610 (formerly sold as a Seradyn product).
Target Information
Apolipoprotein B (apo B) in human plasma is a major protein of low density lipoproteins (LDL) with a molecular mass of approximately 260-500 kDa. Apolipoprotein B binds to specific receptors on cell membranes and is involved in removal of LDL and very low density lipoprotein (VLDL) cholesterol from circulation. Low-density lipoprotein (LDL) is the carrier protein for cholesterol in the blood. LDL binds to its receptor on the capillary walls and thereby mediates the uptake and clearance of cholesterol from the circulation. In atherosclerotic lesions oxidatively modified LDL is found and oxidized LDL is specifically recognized and ingested by macrophages via scavenger receptor A and CD36. Oxidized LDL may be a marker of atherosclerosis but the precise changes in oxidized LDL are not well described. MDA-oxidized LDL appear to be different from LDL oxidized by other means. Apolipoprotein B is mostly synthesized in the liver, and is a major apolipoprotein of very low density, intermediate density and low density lipoproteins (LDL) as well as being a major component of lipoprotein (a). Apolipoprotein B is a ligand for the LDL receptor and elevated levels are associated with premature atherosclerosis. Normal plasma apolipoprotein B levels are around 800mg/L. Apolipoprotein B occurs in plasma as two main isoforms, apoB-48 and apoB-100. The intestinal and the hepatic forms of Apolipoprotein B are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in the Apolipoprotein B gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective Apolipoprotein B, diseases affecting plasma cholesterol and Apolipoprotein B levels.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Apo B-100; Apo B100; apo-B; ApoB 100; ApoB 48; apolipo b; apolipoprotein B (including Ag(x) antigen); Apolipoprotein B 100; Apolipoprotein B 48; Apolipoprotein B-100; Apolipoprotein B100; apolipoprotein B46; apolipoprotein B47; apolipoprotein B48; apolipoprotein B49; mCG_129875; MGC176318
Gene Aliases: APOB; apoB-100; apoB-48; FLDB; LDLCQ4
UniProt ID: (Human) P04114
Entrez Gene ID: (Human) 338
retinoid metabolic process
in utero embryonic development
triglyceride mobilization
receptor-mediated endocytosis
spermatogenesis
nervous system development
cholesterol metabolic process
fertilization
response to virus
response to carbohydrate
post-embryonic development
response to selenium ion
positive regulation of gene expression
positive regulation of macrophage derived foam cell differentiation
positive regulation of lipid storage
positive regulation of cholesterol storage
triglyceride catabolic process
cholesterol transport
sperm motility
response to lipopolysaccharide
cholesterol efflux
low-density lipoprotein particle remodeling
very-low-density lipoprotein particle assembly
low-density lipoprotein particle clearance
lipoprotein metabolic process
lipoprotein biosynthetic process
lipoprotein catabolic process
cholesterol homeostasis
lipoprotein transport
cellular protein catabolic process
regulation of cholesterol biosynthetic process
artery morphogenesis
leukocyte migration
cellular response to tumor necrosis factor
cellular response to prostaglandin stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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