Invitrogen

Apolipoprotein O Monoclonal Antibody (2F1)

6 Published Figures

5 References

View all (5) Apolipoprotein O antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (2)
Published Figures (6)

Apolipoprotein O Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 8

Apolipoprotein O Antibody (MA5-15493) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human testis tissues using ApoO monoclonal antibody (Product # MA5-15493).

Apolipoprotein O Antibody in Western Blot (WB)

Product Image Gallery

Product Details

MA5-15493

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

ELISA (ELISA)

1:10,000

Immunoprecipitation (IP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2F1

Immunogen

Purified recombinant fragment of ApoO expressed in E. Coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_10982238

Product Specific Information

MA5-15493 targets ApoO in indirect ELISA, IHC and WB applications and shows reactivity with Human and mouse samples.

The MA5-15493 immunogen is purified recombinant fragment of ApoO expressed in E. Coli.
.

MA5-15493 detects ApoO which has a predicted molecular weight of approximately 22.2kDa.

Target Information

This gene is a member of the apolipoprotein family. Members of this protein family are involved in the transport and metabolism of lipids. The encoded protein associates with HDL, LDL and VLDL lipoproteins and is characterized by chondroitin-sulfate glycosylation. This protein may be involved in preventing lipid accumulation in the myocardium in obese and diabetic patients. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 3, 4, 5, 12 and 16.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

MIC26 and MIC27 are bona fide subunits of the MICOS complex in mitochondria and do not exist as glycosylated apolipoproteins.

PloS one

Lubeck M,Derkum NH,Naha R,Strohm R,Driessen MD,Belgardt BF,Roden M,Stühler K,Anand R,Reichert AS,Kondadi AK

Published figure using Apolipoprotein O monoclonal antibody (Product # MA5-15493) in Western Blot

Thu Jun 08 00:00:00 EDT 2023

Cristae undergo continuous cycles of membrane remodelling in a MICOS-dependent manner.

EMBO reports

Kondadi AK,Anand R,Hänsch S,Urbach J,Zobel T,Wolf DM,Segawa M,Liesa M,Shirihai OS,Weidtkamp-Peters S,Reichert AS

Published figure using Apolipoprotein O monoclonal antibody (Product # MA5-15493) in Western Blot

Wed Mar 04 00:00:00 EST 2020

MIC26 and MIC27 cooperate to regulate cardiolipin levels and the landscape of OXPHOS complexes.

Life science alliance

Anand R,Kondadi AK,Meisterknecht J,Golombek M,Nortmann O,Riedel J,Peifer-Weiß L,Brocke-Ahmadinejad N,Schlütermann D,Stork B,Eichmann TO,Wittig I,Reichert AS

MA5-15493 was used in Western Blotting to propose that MIC26 and MIC27 apolipoproteins work cooperatively to modulate cardiolipin levels in order to regulate OXPHOS complexes.

Thu Oct 01 00:00:00 EDT 2020

MIC26 and MIC27 cooperate to regulate cardiolipin levels and the landscape of OXPHOS complexes.

Life science alliance

Anand R,Kondadi AK,Meisterknecht J,Golombek M,Nortmann O,Riedel J,Peifer-Weiß L,Brocke-Ahmadinejad N,Schlütermann D,Stork B,Eichmann TO,Wittig I,Reichert AS

MA5-15493 was used in Western Blotting to propose that MIC26 and MIC27 apolipoproteins work cooperatively to modulate cardiolipin levels in order to regulate OXPHOS complexes.

Thu Oct 01 00:00:00 EDT 2020

Mic13 Is Essential for Formation of Crista Junctions in Mammalian Cells.

PloS one

Anand R,Strecker V,Urbach J,Wittig I,Reichert AS

Published figure using Apolipoprotein O monoclonal antibody (Product # MA5-15493) in Western Blot

Tue Aug 22 00:00:00 EDT 2017

The non-glycosylated isoform of MIC26 is a constituent of the mammalian MICOS complex and promotes formation of crista junctions.

Biochimica et biophysica acta

Koob S,Barrera M,Anand R,Reichert AS

MA5-15493 was used in immunoprecipitation and western blot to study the functional role of MIC26 in mitochondria

Wed Jul 01 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: Apolipoprotein O; brain my025; family with sequence similarity 121B; MGC4825; MICOS complex subunit MIC23; MICOS complex subunit MIC26; MYO25; Protein FAM121B

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Gene Aliases: 0610008C08Rik; 1110019O03Rik; APOO; FAM121B; MIC23; MIC26; My025; UNQ1866/PRO4302

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UniProt ID: (Human) Q9BUR5, (Mouse) Q9DCZ4

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Entrez Gene ID: (Human) 79135, (Mouse) 68316

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Function(s)

protein binding molecular_function

Process(es)

biological_process lipid transport cristae formation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Apolipoprotein O Monoclonal Antibody (2F1)

Apolipoprotein O Antibody (MA5-15493) in IHC (P)

Product Details

MA5-15493

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

ELISA (ELISA)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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