Invitrogen

B-Raf Polyclonal Antibody

View all (97) B-Raf antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-85114

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:10,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000
-

Immunoprecipitation (IP)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human B-Raf.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.21 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2792262

Product Specific Information

Keep as concentrated solution.

Positive Control: A549, H1299, HCT116, PC-12.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

BRAF is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It is activated by somatic point mutation in human cancer, and is thought to play a role in the postsynaptic responses of hippocampal neuron. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in B-Raf have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for B-Raf.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 94 kDa B-raf protein; B-Raf proto-oncogene serine/threonine-protein kinase (p94); FLJ95109; MGC126806; murine sarcoma viral (v-raf) oncogene homolog B1; p94; Proto-oncogene B-Raf; Serine/threonine-protein kinase B-raf; v-raf murine sarcoma viral oncogene homolog B; v-Raf murine sarcoma viral oncogene homolog B1

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Gene Aliases: 9930012E13Rik; AA120551; AA387315; AA473386; B-raf; B-RAF1; BRAF; Braf-2; BRAF1; Braf2; C230098H17; C87398; D6Ertd631e; NS7; RAFB1

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UniProt ID: (Human) P15056, (Mouse) P28028

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Entrez Gene ID: (Human) 673, (Rat) 114486, (Mouse) 109880

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Function(s)
protein kinase activity protein serine/threonine kinase activity MAP kinase kinase kinase activity calcium ion binding protein binding ATP binding small GTPase binding mitogen-activated protein kinase kinase binding identical protein binding protein heterodimerization activity nucleotide binding receptor signaling protein activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups metal ion binding non-receptor serine/threonine protein kinase
Process(es)
MAPK cascade activation of MAPKK activity myeloid progenitor cell differentiation protein phosphorylation visual learning organ morphogenesis positive regulation of gene expression negative regulation of fibroblast migration glucose transport thyroid gland development positive regulation of peptidyl-serine phosphorylation somatic stem cell population maintenance cellular response to drug regulation of cell proliferation negative regulation of apoptotic process CD4-positive, alpha-beta T cell differentiation positive T cell selection response to peptide hormone negative regulation of neuron apoptotic process thymus development positive regulation of axon regeneration positive regulation of axonogenesis protein heterooligomerization positive regulation of stress fiber assembly response to cAMP long-term synaptic potentiation head morphogenesis face development positive regulation of ERK1 and ERK2 cascade cellular response to calcium ion establishment of protein localization to membrane positive regulation of substrate adhesion-dependent cell spreading negative regulation of synaptic vesicle exocytosis negative regulation of endothelial cell apoptotic process cell differentiation alpha-beta T cell differentiation regulation of axon regeneration signal transduction phosphorylation intracellular signal transduction
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