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Invitrogen
B-Raf Monoclonal Antibody (1H12)
Product Details
MA5-15495
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
1H12
Immunogen
Purified recombinant fragment of human BRAF expressed in E. Coli.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.03% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_10986987
Product Specific Information
MA5-15495 targets BRAF in indirect ELISA, IF, IHC, and WB applications and shows reactivity with Human and mouse samples.
The MA5-15495 immunogen is purified recombinant fragment of human BRAF expressed in E. Coli.
.
MA5-15495 detects BRAF which has a predicted molecular weight of approximately 87kDa.
Target Information
BRAF is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It is activated by somatic point mutation in human cancer, and is thought to play a role in the postsynaptic responses of hippocampal neuron. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in B-Raf have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for B-Raf.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 94 kDa B-raf protein; B-Raf proto-oncogene serine/threonine-protein kinase (p94); FLJ95109; MGC126806; murine sarcoma viral (v-raf) oncogene homolog B1; p94; Proto-oncogene B-Raf; Serine/threonine-protein kinase B-raf; v-raf murine sarcoma viral oncogene homolog B; v-Raf murine sarcoma viral oncogene homolog B1
Gene Aliases: 9930012E13Rik; AA120551; AA387315; AA473386; B-raf; B-RAF1; BRAF; Braf-2; BRAF1; Braf2; C230098H17; C87398; D6Ertd631e; NS7; RAFB1
UniProt ID: (Human) P15056, (Mouse) P28028
Entrez Gene ID: (Human) 673, (Mouse) 109880
protein kinase activity
protein serine/threonine kinase activity
MAP kinase kinase kinase activity
calcium ion binding
protein binding
ATP binding
small GTPase binding
mitogen-activated protein kinase kinase binding
identical protein binding
protein heterodimerization activity
nucleotide binding
receptor signaling protein activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
metal ion binding
non-receptor serine/threonine protein kinase
MAPK cascade
activation of MAPKK activity
myeloid progenitor cell differentiation
protein phosphorylation
visual learning
organ morphogenesis
positive regulation of gene expression
negative regulation of fibroblast migration
glucose transport
thyroid gland development
positive regulation of peptidyl-serine phosphorylation
somatic stem cell population maintenance
cellular response to drug
regulation of cell proliferation
negative regulation of apoptotic process
CD4-positive, alpha-beta T cell differentiation
positive T cell selection
response to peptide hormone
negative regulation of neuron apoptotic process
thymus development
positive regulation of axon regeneration
positive regulation of axonogenesis
protein heterooligomerization
positive regulation of stress fiber assembly
response to cAMP
long-term synaptic potentiation
head morphogenesis
face development
positive regulation of ERK1 and ERK2 cascade
cellular response to calcium ion
establishment of protein localization to membrane
positive regulation of substrate adhesion-dependent cell spreading
negative regulation of synaptic vesicle exocytosis
negative regulation of endothelial cell apoptotic process
signal transduction
phosphorylation
cell differentiation
intracellular signal transduction
alpha-beta T cell differentiation
regulation of axon regeneration
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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