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BAF53A Antibody (IHC-00287) in IHC
Detection of human BAF53A by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-BAF53A (Cat. No. IHC-00287) used at a dilution of 1:100. Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody used at a dilution of 1:100.
Product Details
IHC-00287
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Between 25 and 75
Conjugate
Form
Liquid
Concentration
0.20 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.0 to 8.0, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Wet ice
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Rat, Chicken, Turkey, Bovine, Dog, Horse, Rabbit, Guinea pig_10141, Pig, Panda, Orangutan, Monkey, Gorilla and Chimpanzee
Target Information
BAF53A is a family member of actin-related proteins (ARPs), which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a 53 kDa subunit protein of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix. Three transcript variants that encode two different protein isoforms have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 53 kDa BRG1-associated factor A; actin-like 6; actin-like 6A; actin-like 6B; Actin-like protein 6A; actin-related protein 4; Actin-related protein Baf53a; ACTL6B; ArpNbeta; BAF complex 53 kDa subunit; BAF53; BAF53A; BRG1-associated factor 53A; BRG1/brm-associated factor 53A; hArpN alpha; hArpN beta; INO80 complex subunit K; MGC5382; MGC5382 Arp4
Gene Aliases: 2810432C06Rik; ACTL6; ACTL6A; AI851094; ARP4; ARPN-BETA; BAF53; BAF53A; C79802; INO80K
UniProt ID: (Human) O96019, (Mouse) Q9Z2N8
Entrez Gene ID: (Human) 86, (Mouse) 56456
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II distal enhancer sequence-specific DNA binding
chromatin binding
transcription coactivator activity
protein binding
nucleosomal DNA binding
actin and actin related protein
actin or actin-binding cytoskeletal protein
cytoskeletal protein
neural retina development
DNA repair
DNA recombination
chromatin remodeling
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
signal transduction
spinal cord development
regulation of growth
ATP-dependent chromatin remodeling
histone H4 acetylation
histone H2A acetylation
regulation of mitophagy
positive regulation of protein targeting to mitochondrion
regulation of transcription, DNA-templated
cellular response to DNA damage stimulus
nervous system development
chromatin modification
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