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BAF53A/BAF53B Antibody (PA5-18272) in ICC/IF
Immunocytochemistry analysis of BAF53A/BAF53B using BAF53A/BAF53B Polyclonal Antibody (Product # PA5-18272) in paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmu... View More
Product Details
PA5-18272
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide sequence (YEEGGKQCVERKCP) corresponding to the C-terminus amino acids of ACTL6A / ACTL6B
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Ammonium sulfate precipitation
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_10985710
Product Specific Information
This antibody is predicted to react with bovine, canine, mouse, porcine and rat based on sequence homology.
This antibody is tested in Peptide ELISA: antibody detection limit dilution 16,000.
Target Information
The protein encoded by this gene is a member of a family of actin-related proteins which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a subunit of the BAF complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. This subunit may be involved in the regulation of genes by structural modulation of their chromatin, specifically in the brain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 53 kDa BRG1-associated factor A; 53 kDa BRG1-associated factor B; actin-like 6; actin-like 6A; Actin-like protein 6A; Actin-like protein 6B; actin-like protein 6B {ECO:0000250|UniProtKB:O94805}; actin-related protein 4; Actin-related protein Baf53a; Actin-related protein Baf53b; actl6b {ECO:0000250|UniProtKB:O94805}; ArpN-alpha; ArpNa; ArpNalpha; ArpNbeta; BAF complex 53 kDa subunit; BAF53; BAF53A; BAF53B; BRG1-associated factor 53A; BRG1-associated factor 53B; BRG1/brm-associated factor 53A; hArpN alpha; hArpN beta; INO80 complex subunit K; MGC5382 Arp4
Gene Aliases: 2810432C06Rik; ACTL6; ACTL6A; ACTL6B; AI851094; ARP4; ARPN-BETA; ArpNa; arpNalpha; BAF53; BAF53A; BAF53B; C79802; INO80K; RGD1307747
UniProt ID: (Human) O96019, (Human) O94805, (Mouse) Q9Z2N8, (Rat) P86173, (Mouse) Q99MR0
Entrez Gene ID: (Human) 86, (Human) 51412, (Mouse) 56456, (Rat) 361925, (Rat) 288563, (Mouse) 83766
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II distal enhancer sequence-specific DNA binding
chromatin binding
transcription coactivator activity
protein binding
nucleosomal DNA binding
structural constituent of cytoskeleton
molecular_function
actin and actin related protein
actin or actin-binding cytoskeletal protein
cytoskeletal protein
neural retina development
DNA repair
DNA recombination
chromatin remodeling
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
signal transduction
spinal cord development
regulation of growth
ATP-dependent chromatin remodeling
histone H4 acetylation
histone H2A acetylation
regulation of mitophagy
positive regulation of protein targeting to mitochondrion
chromatin organization
cytoskeleton organization
covalent chromatin modification
regulation of transcription, DNA-templated
cellular response to DNA damage stimulus
nervous system development
chromatin modification
chromatin assembly or disassembly
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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