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BAG3 Antibody (PA5-144178) in IP
Immunoprecipitation analysis of BAG3 in K562 whole cell lysate; Lane 1: Rabbit control IgG (1 µg). Samples were incubated with BAG3 polyclonal antibody (Product # PA5-144178). For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: BAG3 (6 µg) + K562 whole cell lysate (500 µg). Lane 3: K562 whole cell lysate (10 µg).
Product Details
PA5-144178
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Mouse BAG family molecular chaperone regulator 3 protein (2-577AA)
Conjugate
Form
Liquid
Concentration
5 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.03% Proclin 300
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Wet ice
RRID
AB_3092319
Target Information
BAG3 are among BAG proteins that compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BAG family molecular chaperone regulator 3; BAG-3; Bcl-2-associated athanogene 3; Bcl-2-binding protein Bis; Bcl-2-interacting death suppressor; DKFZp434E0610; MGC104307
Gene Aliases: AA407278; Bag3; Bis; mg638; MNCb-2243
UniProt ID: (Mouse) Q9JLV1
Entrez Gene ID: (Mouse) 29810
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