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BAG3 Antibody (MA5-35563) in IHC (P)
Immunohistochemistry analysis of BAG3 in paraffin-embedded mouse kidney. Samples were incubated with BAG3 Monoclonal antibody (Product # MA5-35563) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Product Details
MA5-35563
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
ARC1104
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human BAG3 (O95817)
Conjugate
Form
Liquid
Concentration
0.25 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2849463
Product Specific Information
Immunogen sequence: NDPRVPSEGP KETPSSANGP SREGSRLPPA REGHPVYPQL RPGYIPIPVL HEGAENRQVH PFHVYPQPGM QRFRTEAAAA APQRSQSPLR GMPETTQPDK Q
Target Information
BAG3 are among BAG proteins that compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BAG family molecular chaperone regulator 3; BAG-3; Bcl-2-associated athanogene 3; Bcl-2-binding protein Bis; Bcl-2-interacting death suppressor; BCL2-associated athanogene 3; BCL2-binding athanogene 3; DKFZp434E0610; Docking protein CAIR-1; MGC104307
Gene Aliases: AA407278; BAG-3; BAG3; BIS; CAIR-1; MFM6; mg638; MNCb-2243
UniProt ID: (Human) O95817, (Mouse) Q9JLV1
Entrez Gene ID: (Human) 9531, (Mouse) 29810
apoptotic process
extrinsic apoptotic signaling pathway via death domain receptors
negative regulation of striated muscle cell apoptotic process
protein stabilization
cellular response to mechanical stimulus
extrinsic apoptotic signaling pathway in absence of ligand
protein folding
brain development
spinal cord development
negative regulation of apoptotic process
regulation of catalytic activity
cell-cell adhesion
regulation of cellular response to heat
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