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FIGURE: 1 / 1
BAG5 Antibody (CF810640) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BAG5 (RC208518, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-BAG5. (1. Positive lysates LY417691 (100 µg) and LC417691 (20 µg) can be purchased separately from OriGene.
Product Details
CF810640
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI2H6
Immunogen
Full length human recombinant protein of human BAG5 produced in 293T.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
It has been hypothesizes that the BAG-5 protein will induce the death of nigral neurons through its predicted interaction with hsp70, which will cause increased protein aggregation and cell death by disinhibition of hsp70's anti-apoptotic function. It is believed that BAG-5 will play an important role in the mechanisms of neuronal death. BAG-5 may also be of interest due to its possible role as a modulator of the hsp70/hsp40 chaperone axis or its possible interaction and coordination of localization/ modulation of other BAG containing proteins via BAG-BAG heterodimerization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BAG family molecular chaperone regulator 5; BAG-5; BAG-family molecular chaperone regulator-5; BAG-family molecular chaperone regulator-5 (BAG-5); Bcl-2-associated athanogene 5; BCL2-associated athanogene 5
Gene Aliases: 1600025G07Rik; 4930405J06Rik; BAG-5; BAG5; KIAA0873
UniProt ID: (Human) Q9UL15, (Rat) Q5QJC9, (Mouse) Q8CI32
Entrez Gene ID: (Human) 9529, (Rat) 366734, (Mouse) 70369
negative regulation of protein ubiquitination
negative regulation of proteasomal ubiquitin-dependent protein catabolic process
negative regulation of ubiquitin-protein transferase activity
negative regulation of protein refolding
neuron death
regulation of inclusion body assembly
negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway
protein folding
Golgi organization
negative regulation of neuron projection development
regulation of ubiquitin-protein transferase activity
regulation of cellular response to heat
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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