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BASP1 Antibody (PA5-114894) in ICC/IF
Immunocytochemistry analysis of BASP1 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-114894) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Product Details
PA5-114894
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human BASP1(Accession P80723), corresponding to amino acid residues M1-E39.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2899530
Product Specific Information
Antibody detects endogenous levels of total BASP1.
Target Information
BASP1 (brain acid soluble protein 1) is a membrane bound protein with several transient phosphorylation sites and PEST motifs. Conservation of proteins with PEST sequences among different species supports their functional significance. PEST sequences typically occur in proteins with high turnover rates. Immunological characteristics of this protein are species specific. This protein also undergoes N-terminal myristoylation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 22 kDa neuronal tissue-enriched acidic protein; brain abundant, membrane attached signal protein 1; Brain acid soluble protein 1; brain acid-soluble protein 1; brain acidic membrane protein; cytoskeleton associated protein 3; MGC8555; Neuronal axonal membrane protein NAP-22; neuronal tissue-enriched acidic protein
Gene Aliases: 2610024P12Rik; BASP1; CAP-23; CAP23; Ckap3; NAP-22; NAP22
UniProt ID: (Human) P80723, (Mouse) Q91XV3, (Rat) Q05175
Entrez Gene ID: (Human) 10409, (Mouse) 70350, (Rat) 64160
negative regulation of transcription, DNA-templated
glomerular visceral epithelial cell differentiation
thorax and anterior abdomen determination
gonad development
substantia nigra development
mesenchymal to epithelial transition
positive regulation of heart growth
diaphragm development
metanephric mesenchyme development
positive regulation of metanephric ureteric bud development
Performance Guarantee
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