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FIGURE: 1 / 1
BHMT Antibody (MA5-35657) in WB
Western blot analysis of BHMT in extracts of various cell lines. Samples were incubated with BHMT Monoclonal antibody (Product # MA5-35657) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Product Details
MA5-35657
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
ARC1249
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 300-406 of human BHMT (Q93088)
Conjugate
Form
Liquid
Concentration
0.56 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2849557
Product Specific Information
Immunogen sequence: CGFEPYHIRA IAEELAPERG FLPPASEKHG SWGSGLDMHT KPWVRARARK EYWENLRIAS GRPYNPSMSK PDGWGVTKGT AELMQQKEAT TEQQLKELFE KQKFKSQ
Target Information
This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Betaine--homocysteine S-methyltransferase 1; betaine-homocysteine methyltransferase; epididymis secretory sperm binding protein Li 61p
Gene Aliases: BHMT; BHMT1; HEL-S-61p
UniProt ID: (Human) Q93088, (Rat) O09171, (Mouse) O35490
Entrez Gene ID: (Human) 635, (Rat) 81508, (Mouse) 12116
sulfur amino acid metabolic process
protein methylation
amino-acid betaine metabolic process
amino-acid betaine catabolic process
choline catabolic process
S-adenosylmethionine metabolic process
regulation of homocysteine metabolic process
L-methionine salvage
methionine biosynthetic process
response to organonitrogen compound
S-methylmethionine cycle
methylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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