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BRD3 Antibody (PA5-30263) in ICC/IF
Immunofluorescence analysis of BRD3 was performed using 70% confluent log phase NTERA-2 cl.D1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with BRD3 Polyclonal Antibody (Product # PA5-30263) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then with Goat anti-Rabbit IgG (Heavy Chain), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 conjugate (Prod... View More
Product Details
PA5-30263
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein fragment corresponding to a region within amino acids 356 and 506 of Human BRD3
Conjugate
Form
Liquid
Concentration
0.54 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2547737
Product Specific Information
Recommended positive controls: HeLa nucleus, mouse Liver.
Predicted reactivity: Rat (93%), Rhesus Monkey (98%), Bovine (81%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
This gene was identified based on its homology to the gene encoding the RING3 protein, a serine/threonine kinase. The gene localizes to 9q34, a region which contains several major histocompatibility complex (MHC) genes. The function of the encoded protein is not known.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: bromodomain-containing 3; Bromodomain-containing FSH-like protein FSRG2; Bromodomain-containing protein 3; bromodomain-containing protein 3 short isoform; RING3-like protein
Gene Aliases: 2410084F24Rik; AW060456; BRD3; Fsrg2; KIAA0043; ORFX; RING3L; RINGL3
UniProt ID: (Human) Q15059, (Mouse) Q8K2F0
Entrez Gene ID: (Human) 8019, (Mouse) 67382
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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