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BRD4-S Antibody (PA5-116913) in WB
Western blot analysis of BRD4-S in extracts from normal (control) and BRD4 Rabbit pAb knockout (KO) HeLa cells. Samples were incubated with BRD4-S Polyclonal antibody (Product # PA5-116913) using a dilution of 1:500, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
Product Details
PA5-116913
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 600-722 of human BRD4 (NP_0551141)
Conjugate
Form
Liquid
Concentration
1.65 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2901543
Product Specific Information
Positive Samples: HeLa, HeLa-BRD4-FL, HeLa-BRD4-S(a), HeLa-BRD4-S(b)
Immunogen sequence: ESEEEDKCKP MSYEEKRQLS LDINKLPGEK LGRVVHIIQS REPSLKNSNP DEIEIDFETL KPSTLRELER YVTSCLRKKR KPQAEKVDVI AGSSKMKGFS SSESESSSES SSSDSEDSET GPA
Target Information
The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: bromodomain-containing 4; Bromodomain-containing protein 4; chromosome-associated protein; Protein HUNK1
Gene Aliases: BRD4; CAP; HUNK1; HUNKI; MCAP
UniProt ID: (Human) O60885
Entrez Gene ID: (Human) 23476, (Rat) 362844
regulation of transcription involved in G1/S transition of mitotic cell cycle
transcription, DNA-templated
cellular response to DNA damage stimulus
positive regulation of G2/M transition of mitotic cell cycle
viral process
covalent chromatin modification
positive regulation of transcription elongation from RNA polymerase II promoter
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of transcription from RNA polymerase II promoter
regulation of inflammatory response
regulation of phosphorylation of RNA polymerase II C-terminal domain
inner cell mass cell proliferation
chromatin remodeling
protein phosphorylation
chromosome segregation
positive regulation of DNA binding
histone H4-K12 acetylation
histone H3-K14 acetylation
negative regulation of DNA damage checkpoint
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