Invitrogen

BRPF1 Polyclonal Antibody

1 Published Figure

5 References

View all (9) BRPF1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (3)
Published Figures (1)

BRPF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 4

BRPF1 Antibody (PA5-27783) in IHC (P)

BRPF1 Polyclonal Antibody detects Peregrin protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. Peregrin stained by BRPF1 Polyclonal Antibody (Product # PA5-27783) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

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Product Details

PA5-27783

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

ChIP assay (ChIP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 318 of Human BRPF1

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.19 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2545259

Product Specific Information

Recommended positive controls: 293T, BRPF1 tansfected 293T cell.

Predicted reactivity: Mouse (97%), Rat (96%), Chicken (89%), Bovine (99%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

BRPF1 (peregrin) is a component of the MOZ/MORF complex. This complex has histone H3 acetyltransferase activity. BRPF1 preferentially mediates histone H3-K23 acetylation and positively regulates the transcription of RUNX1 and RUNX2. Mutations affecting the gene can result in intellectual developmental disorder with dysmorphic facies and ptosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

MOZ and Menin-MLL Complexes Are Complementary Regulators of Chromatin Association and Transcriptional Output in Gastrointestinal Stromal Tumor.

Cancer discovery

Hemming ML,Benson MR,Loycano MA,Anderson JA,Andersen JL,Taddei ML,Krivtsov AV,Aubrey BJ,Cutler JA,Hatton C,Sicinska E,Armstrong SA

PA5-27783 was used in Immunohistochemistry (Paraffin), Western Blot, ChIP Assay to identify complementary chromatin-modifying complexes essential to GIST and characterize the consequences of their disruption, elucidating a novel therapeutic approach to this disease.

Wed Jul 06 00:00:00 EDT 2022

The transcriptional regulator Zfat is essential for maintenance and differentiation of the adipocytes.

Journal of cellular biochemistry

Ishikura S,Nagai M,Tsunoda T,Nishi K,Tanaka Y,Koyanagi M,Shirasawa S

PA5-27783 was used in Western Blotting to report important roles of Zfat in the adipocytes.

Tue Jun 01 00:00:00 EDT 2021

The transcriptional regulator Zfat is essential for maintenance and differentiation of the adipocytes.

Journal of cellular biochemistry

Ishikura S,Nagai M,Tsunoda T,Nishi K,Tanaka Y,Koyanagi M,Shirasawa S

PA5-27783 was used in Western Blotting to report important roles of Zfat in the adipocytes.

Tue Jun 01 00:00:00 EDT 2021

Truncated BRPF1 Cooperates with Smoothened to Promote Adult Shh Medulloblastoma.

Cell reports

Aiello G,Ballabio C,Ruggeri R,Fagnocchi L,Anderle M,Morassut I,Caron D,Garilli F,Gianno F,Giangaspero F,Piazza S,Romanel A,Zippo A,Tiberi L

PA5-27783 was used in Immunohistochemistry to investigate if Shh pathway activation through SmoM2 expression promotes postmitotic neurons dedifferentiation.

Tue Dec 17 00:00:00 EST 2019

Histone Acetyltransferase KAT6A Upregulates PI3K/AKT Signaling through TRIM24 Binding.

Cancer research

Lv D,Jia F,Hou Y,Sang Y,Alvarez AA,Zhang W,Gao WQ,Hu B,Cheng SY,Ge J,Li Y,Feng H

PA5-27783 was used in Western blotting to define an essential role of KAT6A in glioma formation, rationalizing its candidacy as a therapeutic target for GBM treatment.

Wed Nov 15 00:00:00 EST 2017

Molecular mechanisms of transcriptional regulation by the nuclear zinc-finger protein Zfat in T cells.

Biochimica et biophysica acta

Ishikura S,Tsunoda T,Nakabayashi K,Doi K,Koyanagi M,Hayashi K,Kawai T,Tanaka Y,Iwaihara Y,Luo H,Nishi K,Okamura T,Shirasawa S

PA527783 was used in western blot to elucidate the mechanisms by which Zfat alters gene expression in T cells

Tue Nov 01 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: Bromodomain and PHD finger-containing protein 1; bromodomain-containing protein, 140kD; Peregrin; Protein Br140

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Gene Aliases: BR140; BRPF1

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UniProt ID: (Human) P55201

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Entrez Gene ID: (Human) 7862

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Function(s)

DNA binding protein binding zinc ion binding DNA-binding transcription factor zinc finger transcription factor gene-specific transcriptional regulator

Process(es)

transcription, DNA-templated histone H3 acetylation positive regulation of transcription, DNA-templated regulation of signal transduction by p53 class mediator

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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BRPF1 Polyclonal Antibody

BRPF1 Antibody (PA5-27783) in IHC (P)

Product Details

PA5-27783

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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