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C9orf89 Antibody (PA5-89068) in WB
Western blot analysis of C9orf89 in extracts of various cell lines. Samples were incubated with C9orf89 Polyclonal antibody (Product # PA5-89068) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Product Details
PA5-89068
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human CARD19 (NP_1156863)
Conjugate
Form
Liquid
Concentration
1.48 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2805333
Product Specific Information
Immunogen sequence: MTDQTYCDRL VQDTPFLTGH GRLSEQQVDR IILQLNRYYP QILTNKEAEK FRNPKASLRV RLCDLLSHLQ RSGERDCQEF YRALYIHAQP LHSRLPSRHA; Positive Samples: 293T, A-549, HeLa, A-431, Mouse kidney; Cellular Location: Endoplasmic reticulum membrane, Mitochondrion membrane, Nucleus, Single-pass membrane protein
Target Information
Plays a role in inhibiting the effects of BCL10-induced activation of NF-kappa-B. May inhibit the phosphorylation of BCL10 in a CARD-dependent manner.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Bcl10-interacting CARD protein; Bcl10-interacting protein with CARD; BinCARD; caspase recruitment domain family, member 19; Caspase recruitment domain-containing protein 19
Gene Aliases: AI851695; BinCARD; C9orf89; CARD19
UniProt ID: (Mouse) Q9D1I2, (Human) Q96LW7
Entrez Gene ID: (Mouse) 68480, (Human) 84270
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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