Proteintech

CA9 Polyclonal Antibody

CA9 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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CA9 Antibody (11071-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human renal cell carcinoma tissue slide using 11071-1-AP ( CA IX antibody at dilution of 1:50 (under 40x lens).

CA9 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

11071-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 15 publications 15 publications

Immunohistochemistry (IHC)

View 22 publications 22 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

CA9 Fusion Protein Ag1540 (31-408 aa encoded by BC014950)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.27 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: LLMPVHPQR LPRMQEDSPL GGGSSGEDDP LGEEDLPSEE DSPREEDPPG EEDLPGEEDL PGEEDLPEVK PKSEEEGSLK LEDLPTVEAP GDPQEPQNNA HRDKEGDDQS HWRYGGDPPW PRVSPACAGR FQSPVDIRPQ LAAFCPALRP LELLGFQLPP LPELRLRNNG HSVQLTLPPG LEMALGPGRE YRALQLHLHW GAAGRPGSEH TVEGHRFPAE IHVVHLSTAF ARVDEALGRP GGLAVLAAFL EEGPEENSAY EQLLSRLEEI AEEGSETQVP GLDISALLPS DFSRYFQYEG SLTTPPCAQG VIWTVFNQTV MLSAKQLHTL SDTLWGPGDS RLQLNFRATQ PLNGRVIEAS FPAGVDSSPR AAEPVQLNS (31-408 aa encoded by BC014950)

Target Information

Carbonic anhydrase (CA) is an enzyme that assists rapid interconversion of carbon dioxide and water into carbonic acid, protons, and bicarbonate ions. It is abundant in all mammalian tissues. There are many genes that are inducible by hypoxia, via HIF-1 alpha. CA IX is one of the most inducible genes because of its stability and location within the membrane. Carbonic anhydrases have a widespread role in regulating pH in normal tissues, by regulating hydrogen ion (H+) flux. The pH is important in cell death under hypoxia, thus a blockade of CA IX results in increased cell death under hypoxia. Therefore, CA IX has become a reliable histochemical marker of hypoxia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CA-9; CA-IX; Carbonate dehydratase IX; Carbonic anhydrase 9; Carbonic anhydrase IX; carbonic dehydratase; Membrane antigen MN; Membrane antigen MN homolog; P54 / 58N; P54/58N; pMW1; RCC-associated antigen G250; RCC-associated protein G250; Renal cell carcinoma-associated antigen G250

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Gene Aliases: CA9; CAIX; Car9; G250; MN; MN/CA9

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UniProt ID: (Human) Q16790, (Mouse) Q8VHB5

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Entrez Gene ID: (Human) 768, (Mouse) 230099, (Rat) 313495

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Function(s)

carbonate dehydratase activity zinc ion binding lyase activity metal ion binding molecular_function

Process(es)

morphogenesis of an epithelium one-carbon metabolic process secretion response to hypoxia response to testosterone response to drug bicarbonate transport regulation of transcription from RNA polymerase II promoter in response to hypoxia

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CA9 Polyclonal Antibody

CA9 Antibody (11071-1-AP) in IHC (P)

Product Details

11071-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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