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Product Details
PA5-97015
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1650-1900 of human CAD (NP_0043322)
Conjugate
Form
Liquid
Concentration
1.36 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2808817
Product Specific Information
Immunogen sequence: LERLGPGKGE VRPELGSRQD VEALWENMAV IDCFASDHAP HTLEEKCGSR PPPGFPGLET MLPLLLTAVS EGRLSLDDLL QRLHHNPRRI FHLPPQEDTY VEVDLEHEWT IPSHMPFSKA HWTPFEGQKV KGTVRRVVLR GEVAYIDGQV LVPPGYGQDV RKWPQGAVPQ LPPSAPATSE MTTTPERPRR GIPGLPDGRF HLPPRIHRAS DPGLPAEEPK EKSSRKVAEP ELMGTPDGTC YPPPPVPRQA S; Positive Samples: SW620, HepG2, HeLa, HL-60; Cellular Location: Cytoplasm, Nucleus
Target Information
The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CAD protein; CAD trifunctional protein; Carbamoyl phosphate synthetase 2-aspartate transcarbamylase-dihydroorotase; carbamyl phosphatate synthetase 2; Multifunctional protein CAD
Gene Aliases: 2410008J01Rik; AU018859; CAD; CDG1Z; Cpad
UniProt ID: (Human) P27708, (Mouse) B2RQC6
Entrez Gene ID: (Human) 790, (Mouse) 69719, (Rat) 24240
aspartate carbamoyltransferase activity
carbamoyl-phosphate synthase (ammonia) activity
carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity
dihydroorotase activity
protein kinase activity
ATP binding
zinc ion binding
enzyme binding
identical protein binding
aspartate binding
nucleotide binding
UTP binding
catalytic activity
amino acid binding
transferase activity
carboxyl- or carbamoyltransferase activity
hydrolase activity
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides
ligase activity
metal ion binding
hydrolase
ligase
urea cycle
liver development
'de novo' pyrimidine nucleobase biosynthetic process
UTP biosynthetic process
arginine biosynthetic process
glutamine metabolic process
heart development
female pregnancy
lactation
response to amine
drug metabolic process
peptidyl-threonine phosphorylation
response to caffeine
organ regeneration
response to testosterone
cellular response to drug
'de novo' UMP biosynthetic process
pyrimidine nucleoside biosynthetic process
protein autophosphorylation
response to cortisol
cellular response to epidermal growth factor stimulus
pyrimidine nucleotide biosynthetic process
cellular amino acid metabolic process
nitrogen compound metabolic process
metabolic process
glutamine catabolic process
carbamoyl phosphate biosynthetic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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