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CARMA1 Antibody (A302-542A) in WB
Detection of human CARMA1 by western blot and immunoprecipitation. Samples: Whole cell lysate from Ramos (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and K562 (K; 50 µg) cells. Antibodies: Affinity purified rabbit anti-CARMA1 antibody A302-542A used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 10 µg/mg lysate. CARMA1 was also immunoprecipitated by rabbit anti-CARMA1 antibodies A302-541A, A302-543A and A302-544A, which recognize other epitopes. Detection: Chemiluminescence with exposure t... View More
Product Details
A302-542A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 475 and 525 of human card-maguk protein 1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Wet ice
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Mouse
Target Information
The protein encoded by this gene belongs to the membrane-associated guanylate kinase (MAGUK) family, a class of proteins that functions as molecular scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. This protein is also a member of the CARD protein family, which is defined by carrying a characteristic caspase-associated recruitment domain (CARD). This protein has a domain structure similar to that of CARD14 protein. The CARD domains of both proteins have been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-kappaB activation. When expressed in cells, this protein activated NF-kappaB and induced the phosphorylation of BCL10.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: bcl10-interacting maguk protein 3; CARD-containing MAGUK protein 1; Carma 1; caspase recruitment domain family, member 11; Caspase recruitment domain-containing protein 11; MGC133069
Gene Aliases: BENTA; BIMP3; CARD11; CARMA1; IMD11; PPBL
UniProt ID: (Human) Q9BXL7
Entrez Gene ID: (Human) 84433
positive regulation of cytokine production
stimulatory C-type lectin receptor signaling pathway
positive regulation of B cell proliferation
T cell costimulation
Fc-epsilon receptor signaling pathway
positive regulation of T cell proliferation
regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
thymic T cell selection
positive regulation of interleukin-2 biosynthetic process
regulation of B cell differentiation
regulation of T cell differentiation
GMP metabolic process
GDP metabolic process
T cell receptor signaling pathway
positive regulation of NF-kappaB transcription factor activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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