Invitrogen

CCL2 (MCP-1) Monoclonal Antibody (2H5), PE, eBioscience™

1 Published Figure
2 References
View all (67) CCL2 (MCP-1) antibodies
Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

12-7096-82

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

0.25 µg/test
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Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Armenian hamster / IgG

Recommended Isotype Control

Armenian Hamster IgG Isotype Control (eBio299Arm), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

2H5

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466171

Product Specific Information

Description: The 2H5 monoclonal antibody reacts with mouse, rat, and human monocyte chemoattractant protein-1 (MCP-1), also known as CCL2 and MCAF.

Applications Reported:The 2H5 antibody has been reported for use in ELISA, intracellular staining for flow cytometric analysis, and neutralization. (Please use Functional Grade purified 2H5 (Product # 16-7096-81) in functional assays).

Applications Tested: This 2H5 antibody is tested by flow cytometric analysis of fixed permabilized LPS-stimulated mouse thioglycolate-elicited peritoneal exudate cells. Has been tested by flow cytometric analysis of fixed permabilized LPS-stimulated mouse thioglycolate-elicited peritoneal exudate cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Bioinformatics

Protein Aliases: C-C motif chemokine 2; chemokine (C-C motif) ligand 2; H-MCP-1; HC11; immediate-early serum-responsive JE protein; Immediate-early serum-responsive protein JE; MCAF; MCP-1; MGC9434; Monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; Monocyte chemotactic and activating factor; Monocyte chemotactic protein 1; Monocyte secretory protein JE; Platelet-derived growth factor-inducible protein JE; RP23-350G1.3; small inducible cytokine A2; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je); small inducible cytokine subfamily A (Cys-Cys), member 2; small inducible gene JE; Small-inducible cytokine A2

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Gene Aliases: AI323594; CCL2; GDCF-2; HC11; HSMCR30; JE; MCAF; MCP-1; MCP1; SCYA2; Sigje; SMC-CF

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UniProt ID: (Human) P13500, (Rat) P14844, (Mouse) P10148

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Entrez Gene ID: (Human) 6347, (Rat) 24770, (Mouse) 20296

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Function(s)
protein kinase activity receptor binding chemokine activity heparin binding CCR2 chemokine receptor binding G-protein coupled receptor binding cytokine activity protein binding cytokine intercellular signal molecule
Process(es)
MAPK cascade angiogenesis response to hypoxia positive regulation of leukocyte mediated cytotoxicity positive regulation of endothelial cell proliferation leukocyte migration involved in inflammatory response monocyte chemotaxis positive regulation of cellular extravasation protein phosphorylation cellular calcium ion homeostasis chemotaxis inflammatory response humoral immune response cytoskeleton organization cell adhesion signal transduction cell surface receptor signaling pathway transforming growth factor beta receptor signaling pathway G-protein coupled receptor signaling pathway G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger JAK-STAT cascade aging regulation of cell shape response to heat response to wounding response to mechanical stimulus response to bacterium organ morphogenesis response to gamma radiation regulation of vascular endothelial growth factor production positive regulation of macrophage chemotaxis response to activity negative regulation of angiogenesis viral genome replication cytokine-mediated signaling pathway cellular homeostasis neutrophil chemotaxis organ regeneration lipopolysaccharide-mediated signaling pathway response to progesterone positive regulation of tumor necrosis factor production cellular response to insulin stimulus positive regulation of collagen biosynthetic process response to vitamin B3 negative regulation of glial cell apoptotic process helper T cell extravasation cellular response to drug cellular response to macrophage colony-stimulating factor stimulus cellular response to platelet-derived growth factor stimulus PERK-mediated unfolded protein response response to amino acid protein kinase B signaling negative regulation of neuron apoptotic process positive regulation of GTPase activity astrocyte cell migration cellular response to fibroblast growth factor stimulus response to ethanol response to antibiotic vascular endothelial growth factor receptor signaling pathway macrophage chemotaxis lymphocyte chemotaxis positive regulation of inflammatory response positive regulation of synaptic transmission positive regulation of T cell activation positive regulation of nitric-oxide synthase biosynthetic process maternal process involved in female pregnancy maternal process involved in parturition chemokine-mediated signaling pathway positive regulation of ERK1 and ERK2 cascade cellular response to lipopolysaccharide cellular response to retinoic acid cellular response to ATP cellular response to interferon-gamma cellular response to interleukin-1 cellular response to interleukin-6 cellular response to tumor necrosis factor cellular response to high density lipoprotein particle stimulus cellular response to organic cyclic compound cellular response to dexamethasone stimulus positive regulation of monocyte chemotaxis positive regulation of immune complex clearance by monocytes and macrophages positive regulation of calcium ion import positive regulation of protein targeting to membrane positive regulation of apoptotic cell clearance negative regulation of natural killer cell chemotaxis positive regulation of leukocyte migration glial cell migration positive regulation of cell-cell adhesion response to lipopolysaccharide chemokinesis response to drug positive regulation of cell adhesion response to glucocorticoid cellular response to lipoprotein particle stimulus immune response monocyte homeostasis eosinophil chemotaxis
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