Invitrogen

c-Kit Monoclonal Antibody (104D2), APC

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Published Figures (1)

FIGURE: 1 / 1

c-Kit Antibody (CD11705) in Flow

PloS one 2017 - 10.1371/journal.pone.0164457.g002 Fig 2 The efficiency of Definitive Endoderm (DE) and Gut Tube Endoderm formation at the stage 1 and 2 of the differentiation protocol. (A) Flow cytometry, and immunofluorescence staining for DE-specific markers in the differentiated H1 ES cells. (B) Quantitative RT-PCR results for Gut Tube Endoderm-specific markers are shown in (B), showing genes up-regulated in the stage 1... View More

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Product Details

CD11705

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

Assay-Dependent

View 12 publications 12 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

104D2

Immunogen

MOLM-1 megakaryocytic cells

View immunogen

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Purification

purified

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536476

Product Specific Information

Allophycocyanin (APC) is a stable and highly soluble phycobiliprotein that provides maximal absorbance and fluorescence without susceptibility to internal or external fluorescence quenching, thus providing exceptional quantum yields and molar extinction coefficients.

Target Information

KIT (c-KIT) is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). KIT was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT together with its ligand regulates growth and activation of a variety of hemopoietic and non-hemopoietic cells. Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Recently, deregulation of the KIT receptor TK by the prevalent activation loop mutation D816V has served as a focal point in therapeutic strategies aimed at curbing neoplastic mast cell growth. c-Kit is expressed in hematopoietic stem cells, germ cells, mast cells and gastrointestinal tract cajal cells. Upon binding of its ligand stem cell factor (SCF), c-kit dimerizes, resulting in receptor activation and autophosphorylation of various tyrosine residues including tyrosine 703 located on the cytoplasmic domain of the receptor. This modification allows docking of Grb2 and activation of the Ras/ERK signaling pathway. SCF/c-kit can activate multiple downstream signaling pathways including PI3K, PLC-gamma and JAK/STAT. c-kit receptor activation is essential for hematopoiesis, stem cell maintenance and gametogenesis.

Analyte Specific Reagent

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

CRISPR interference interrogation of COPD GWAS genes reveals the functional significance of desmoplakin in iPSC-derived alveolar epithelial cells.

Science advances

Werder RB,Liu T,Abo KM,Lindstrom-Vautrin J,Villacorta-Martin C,Huang J,Hinds A,Boyer N,Bullitt E,Liesa M,Silverman EK,Kotton DN,Cho MH,Zhou X,Wilson AA

CD11705 was used in Flow cytometry/Cell sorting to apply CRISPR interference to interrogate the function of nine genes implicated in COPD by GWAS in induced pluripotent stem cell-derived type 2 alveolar epithelial cells.

Fri Jul 15 00:00:00 EDT 2022

Modeling plasticity and dysplasia of pancreatic ductal organoids derived from human pluripotent stem cells.

Cell stem cell

Breunig M,Merkle J,Wagner M,Melzer MK,Barth TFE,Engleitner T,Krumm J,Wiedenmann S,Cohrs CM,Perkhofer L,Jain G,Krüger J,Hermann PC,Schmid M,Madácsy T,Varga Á,Griger J,Azoitei N,Müller M,Wessely O,Robey PG,Heller S,Dantes Z,Reichert M,Günes C,Bolenz C,Kuhn F,Maléth J,Speier S,Liebau S,Sipos B,Kuster B,Seufferlein T,Rad R,Meier M,Hohwieler M,Kleger A

CD11705 was used in Flow cytometry/Cell sorting to show that PDLOs enable in vitro and in vivo studies of pancreatic plasticity, dysplasia, and cancer formation from a genetically defined background.

Thu Jun 03 00:00:00 EDT 2021

Adenine base editing reduces misfolded protein accumulation and toxicity in alpha-1 antitrypsin deficient patient iPSC-hepatocytes.

Molecular therapy : the journal of the American Society of Gene Therapy

Werder RB,Kaserman JE,Packer MS,Lindstrom-Vautrin J,Villacorta-Martin C,Young LE,Aratyn-Schaus Y,Gregoire F,Wilson AA

CD11705 was used in Flow cytometry/Cell sorting to apply adenine base editors to correct the Z mutation, a single-base substitution that leads to AAT protein misfolding and associated liver and lung disease, in patient induced pluripotent stem cells (iPSCs) and iPSC-derived hepatocytes (iHeps).

Wed Nov 03 00:00:00 EDT 2021

Transcriptional changes and the role of ONECUT1 in hPSC pancreatic differentiation.

Communications biology

Heller S,Li Z,Lin Q,Geusz R,Breunig M,Hohwieler M,Zhang X,Nair GG,Seufferlein T,Hebrok M,Sander M,Julier C,Kleger A,Costa IG

CD11705 was used in Flow Cytometry to suggest that ONECUT1 is also involved in preparing pancreatic progenitors for later endocrine specification.

Wed Nov 17 00:00:00 EST 2021

CDKN2A-Mutated Pancreatic Ductal Organoids from Induced Pluripotent Stem Cells to Model a Cancer Predisposition Syndrome.

Cancers

Merkle J,Breunig M,Schmid M,Allgöwer C,Krüger J,Melzer MK,Bens S,Siebert R,Perkhofer L,Azoitei N,Seufferlein T,Heller S,Meier M,Müller M,Kleger A,Hohwieler M

CD11705 was used in Flow Cytometry to provide a model for hereditary human pancreatic cancer that enables dissection of tumor initiation and early development starting from patient-specific CDKN2A-mutated pluripotent stem cells.

Wed Oct 13 00:00:00 EDT 2021

Generation of pulmonary neuroendocrine cells and SCLC-like tumors from human embryonic stem cells.

The Journal of experimental medicine

Chen HJ,Poran A,Unni AM,Huang SX,Elemento O,Snoeck HW,Varmus H

CD11705 was used in Flow Cytometry to demonstrate that inhibition of NOTCH signaling induces up to 10% of lung progenitor cells to form pulmonary neuroendocrine cells (PNECs), putative precursors to small cell lung cancers (SCLCs), and PNECs can be increased by reducing levels of retinoblastoma (RB) proteins with inhibitory RNA.

Mon Mar 04 00:00:00 EST 2019

Essential Gene Profiles for Human Pluripotent Stem Cells Identify Uncharacterized Genes and Substrate Dependencies.

Cell reports

Mair B,Tomic J,Masud SN,Tonge P,Weiss A,Usaj M,Tong AHY,Kwan JJ,Brown KR,Titus E,Atkins M,Chan KSK,Munsie L,Habsid A,Han H,Kennedy M,Cohen B,Keller G,Moffat J

CD11705 was used in Flow cytometry/Cell sorting to establish parameters for genome-wide screens in hPSCs to facilitate the characterization of unappreciated genetic regulators of hPSC biology.

Tue Apr 09 00:00:00 EDT 2019

Notch and Aryl Hydrocarbon Receptor Signaling Impact Definitive Hematopoiesis from Human Pluripotent Stem Cells.

Stem cells (Dayton, Ohio)

Leung A,Zulick E,Skvir N,Vanuytsel K,Morrison TA,Naing ZH,Wang Z,Dai Y,Chui DHK,Steinberg MH,Sherr DH,Murphy GJ

CD11705 was used in Flow cytometry/Cell sorting to show that genetic or small molecule inhibition of the AHR results in the increased production of CD34+ CD45+ HSPCs while conversely, activation of the same receptor results in a block of hematopoietic cell emergence.

Sun Jul 01 00:00:00 EDT 2018

Derivation of Epithelial-Only Airway Organoids from Human Pluripotent Stem Cells.

Current protocols in stem cell biology

McCauley KB,Hawkins F,Kotton DN

CD11705 was used in Flow cytometry/Cell sorting to identify a way to efficiently generate expandable airway epithelial organoids from human pluripotent stem cells.

Tue May 01 00:00:00 EDT 2018

Derivation of Epithelial-Only Airway Organoids from Human Pluripotent Stem Cells.

Current protocols in stem cell biology

McCauley KB,Hawkins F,Kotton DN

CD11705 was used in Flow cytometry/Cell sorting to identify a way to efficiently generate expandable airway epithelial organoids from human pluripotent stem cells.

Tue May 01 00:00:00 EDT 2018

Generation of polyhormonal and multipotent pancreatic progenitor lineages from human pluripotent stem cells.

Methods (San Diego, Calif.)

Korytnikov R,Nostro MC

CD11705 was used in flow cytometry to describe protocols to generate specific pancreatic lineages from hPSCs

Sun May 15 00:00:00 EDT 2016

An Abbreviated Protocol for In Vitro Generation of Functional Human Embryonic Stem Cell-Derived Beta-Like Cells.

PloS one

Massumi M,Pourasgari F,Nalla A,Batchuluun B,Nagy K,Neely E,Gull R,Nagy A,Wheeler MB

CD11705 was used in flow cytometry to describe a method to produce human embryonic stem cell-derived Beta-like cells

Wed May 31 00:00:00 EDT 2017

An Abbreviated Protocol for In Vitro Generation of Functional Human Embryonic Stem Cell-Derived Beta-Like Cells.

PloS one

Massumi M,Pourasgari F,Nalla A,Batchuluun B,Nagy K,Neely E,Gull R,Nagy A,Wheeler MB

CD11705 was used in flow cytometry to describe a method to produce human embryonic stem cell-derived Beta-like cells

Wed May 31 00:00:00 EDT 2017

SOX17 is a critical specifier of human primordial germ cell fate.

Cell

Irie N,Weinberger L,Tang WW,Kobayashi T,Viukov S,Manor YS,Dietmann S,Hanna JH,Surani MA

CD11705 was used in flow cytometry to identify SOX17 as a key regulator of human primordial germ cell fate.

Thu Jan 15 00:00:00 EST 2015

The in vitro generation of lung and airway progenitor cells from human pluripotent stem cells.

Nature protocols

Huang SX,Green MD,de Carvalho AT,Mumau M,Chen YW,D'Souza SL,Snoeck HW

CD11705 was used in Immunofluorescence to describe a strategy for directed differentiation of hPSCs into developmental lung progenitors, and their subsequent differentiation into predominantly distal lung epithelial cells.

Sun Mar 01 00:00:00 EST 2015

Bioinformatics

Protein Aliases: CD117; ckit; Mast/stem cell growth factor receptor Kit; p145 c-kit; PBT; Piebald trait protein; Proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SCFR; soluble KIT variant 1; Tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

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Gene Aliases: C-Kit; CD117; KIT; PBT; SCFR

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UniProt ID: (Human) P10721

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Entrez Gene ID: (Human) 3815

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Function(s)

protease binding protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity stem cell factor receptor activity Ras guanyl-nucleotide exchange factor activity protein binding ATP binding cytokine binding protein homodimerization activity metal ion binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity transmembrane signal receptor

Process(es)

Performance Guarantee

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c-Kit Monoclonal Antibody (104D2), APC

c-Kit Antibody (CD11705) in Flow

Product Details

CD11705

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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