Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 9
CD34 Antibody (PA5-78978) in IHC (P)
Immunohistochemical analysis of CD34 in paraffin-embedded section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-CD34 antibody (Product # PA5-78978) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used... View More
Product Details
PA5-78978
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human CD34 (366-382aa QATSRNGHSARQHVVAD).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.01mg sodium azide, 0.05mg thimerosal
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2746094
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
CD34 is a highly glycosylated monomeric with a molecular weight range of 111-115 kDa surface protein that is present on many stem cell populations. CD34 is a stem cell marker although its expression on human hematopoietic stem cells is reversible. CD34 may serve as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions. CD34 is possibly an adhesion molecule with a putative role for mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Further, CD34 could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C suggesting a putative role in signal transduction. Diseases associated with CD34 dysfunction include dermatofibrosarcoma and neurofibroma.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD34; CD34 antigen; cluster designation 34; Hematopoietic progenitor cell antigen CD34; Mucosialin; RP11-328D5.2
Gene Aliases: AU040960; CD34
UniProt ID: (Human) P28906, (Mouse) Q64314
Entrez Gene ID: (Human) 947, (Rat) 305081, (Mouse) 12490
tissue homeostasis
endothelial cell proliferation
glomerular filtration
endothelium development
cell-matrix adhesion
signal transduction
regulation of blood pressure
cell proliferation
positive regulation of gene expression
negative regulation of gene expression
single organismal cell-cell adhesion
hemopoiesis
negative regulation of blood coagulation
negative regulation of tumor necrosis factor production
positive regulation of interleukin-10 production
mesangial cell-matrix adhesion
paracrine signaling
positive regulation of odontogenesis
negative regulation of nitric oxide biosynthetic process
positive regulation of angiogenesis
cell motility
regulation of immune response
leukocyte migration
transdifferentiation
vascular wound healing
hematopoietic stem cell proliferation
positive regulation of transforming growth factor beta production
positive regulation of granulocyte colony-stimulating factor production
extracellular exosome assembly
glomerular endothelium development
stem cell proliferation
metanephric glomerular mesangial cell differentiation
negative regulation of cellular response to heat
negative regulation of cellular response to hypoxia
negative regulation of interleukin-2 secretion
positive regulation of glial cell-derived neurotrophic factor secretion
negative regulation of neuron death
positive regulation of vasculogenesis
cell adhesion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support