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Invitrogen
CD85k (ILT3) Monoclonal Antibody (ZM4.1), PE-Cyanine7, eBioscience™
FIGURE: 1 / 1
CD85k (ILT3) Antibody (25-5139-42) in Flow
Normal human peripheral blood cells were stained with CD123 Monoclonal Antibody, Super Bright 702 (Product # 67-1239-42) and Mouse IgG1 kappa Isotype Control, PE-Cyanine7 (Product # 25-4714-80) (left) or CD85k Monoclonal Antibody, PE-Cyanine7 (right). Cells in the monocyte gate were used for analysis.
Product Details
25-5139-42
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
ZM4.1
Conjugate
Excitation/Emission Max
569/780 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2802255
Product Specific Information
Description: The monoclonal antibody ZM4.1 recognizes ILT3, also known as CD85k. ILT3 is a member of the large family of ILT molecules (Immunologulin-like Transcripts, Leucocyte Ig-like Receptors (LIRs) and Monocyte/Macrophage Ig-like Receptors (MIRs). ILT3 is a 60kDa transmembrane protein with two Ig domains and ITIMs. Expression is found on monocytes, macrophages, and dendritic cells. High levels of ILT3 expression is also found on plasmacytoid dendritic cells (pDCs). ILT3 expression by DCs has been shown to be required to induce Regulatory Tcells (tregs). Addition of ZM4.1, like ZM3.8, blocks the suppressive function of the Tregs in in vitro culture systems with allogenic DC's.
Applications Reported: This ZM4.1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This ZM4.1 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Target Information
This gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4. The encoded protein belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. The receptor can also function in antigen capture and presentation. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B4; CD85 antigen-like family member K; CD85k; ILT-3; Immunoglobulin-like transcript 3; leucocyte Ig-like receptor B4; Leukocyte immunoglobulin-like receptor 5; Leukocyte immunoglobulin-like receptor subfamily B member 4; leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 4; LIR-5; Monocyte inhibitory receptor HM18
Gene Aliases: CD85K; ILT-3; ILT3; LILRB4; LIR-5; LIR5
UniProt ID: (Human) Q8NHJ6
Entrez Gene ID: (Human) 11006
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