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CLOCK Antibody (A302-618A) in WB
Detection of mouse CLOCK by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, CH27, CT26, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CLOCK antibody (Product # A302-618A lot 3) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
Product Details
A302-618A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 796 and 846 of human circadian locomoter output cycles kaput protein.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Wet ice
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Orangutan
Target Information
This gene encodes a protein that belongs to the basic helix-loop-helix (bHLH) family of transcription factors. Polymorphisms within the encoded protein have been associated with circadian rhythm sleep disorders. A similar protein in mice is a circadian regulator that acts as a transcription factor and forms a heterodimer with aryl hydrocarbon receptor nuclear translocator-like to activate transcription of mouse period 1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: bHLHe8; circadian locomoter output cycles kaput protein; Circadian locomoter output cycles protein kaput; Class E basic helix-loop-helix protein 8; clock homolog; hCLOCK; mCLOCK
Gene Aliases: 5330400M04Rik; BHLHE8; CLOCK; KAT13D; KIAA0334
UniProt ID: (Human) O15516, (Mouse) O08785
Entrez Gene ID: (Human) 9575, (Mouse) 12753
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding
core promoter sequence-specific DNA binding
core promoter binding
transcriptional activator activity, RNA polymerase II transcription factor binding
DNA binding
transcription factor activity, sequence-specific DNA binding
histone acetyltransferase activity
protein binding
chromatin DNA binding
sequence-specific DNA binding
protein dimerization activity
E-box binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
signal transducer activity
transcription factor binding
transferase activity
transferase activity, transferring acyl groups
DNA damage checkpoint
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
cellular response to DNA damage stimulus
signal transduction
spermatogenesis
circadian rhythm
histone acetylation
circadian regulation of gene expression
regulation of hair cycle
proteasome-mediated ubiquitin-dependent protein catabolic process
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
rhythmic process
positive regulation of inflammatory response
regulation of insulin secretion
positive regulation of NF-kappaB transcription factor activity
response to redox state
cellular response to ionizing radiation
regulation of type B pancreatic cell development
negative regulation of glucocorticoid receptor signaling pathway
regulation of transcription from RNA polymerase II promoter
photoperiodism
entrainment of circadian clock
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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