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Invitrogen
CLOCK Recombinant Rabbit Monoclonal Antibody (JA12-33)
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 10
CLOCK Antibody (MA5-32713) in ICC/IF
Immunocytochemical analysis of CLOCK in SHG-44 cells using a CLOCK Monoclonal antibody (Product # MA5-32713) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Product Details
MA5-32713
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JA12-33
Immunogen
Synthetic peptide within Human CLOCK aa 140-189
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2809990
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
This gene encodes a protein that belongs to the basic helix-loop-helix (bHLH) family of transcription factors. Polymorphisms within the encoded protein have been associated with circadian rhythm sleep disorders. A similar protein in mice is a circadian regulator that acts as a transcription factor and forms a heterodimer with aryl hydrocarbon receptor nuclear translocator-like to activate transcription of mouse period 1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: bHLHe8; circadian locomoter output cycles kaput; circadian locomoter output cycles kaput protein; Circadian locomoter output cycles protein kaput; Class E basic helix-loop-helix protein 8; clock homolog; hCLOCK; mCLOCK; rCLOCK
Gene Aliases: 5330400M04Rik; BHLHE8; CLOCK; KAT13D; KIAA0334
UniProt ID: (Human) O15516, (Mouse) O08785, (Rat) Q9WVS9
Entrez Gene ID: (Human) 9575, (Mouse) 12753, (Rat) 60447
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding
core promoter sequence-specific DNA binding
core promoter binding
transcriptional activator activity, RNA polymerase II transcription factor binding
DNA binding
transcription factor activity, sequence-specific DNA binding
histone acetyltransferase activity
protein binding
chromatin DNA binding
sequence-specific DNA binding
protein dimerization activity
E-box binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
signal transducer activity
transcription factor binding
transferase activity
transferase activity, transferring acyl groups
DNA damage checkpoint
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
cellular response to DNA damage stimulus
signal transduction
spermatogenesis
circadian rhythm
histone acetylation
circadian regulation of gene expression
regulation of hair cycle
proteasome-mediated ubiquitin-dependent protein catabolic process
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
rhythmic process
positive regulation of inflammatory response
regulation of insulin secretion
positive regulation of NF-kappaB transcription factor activity
response to redox state
cellular response to ionizing radiation
regulation of type B pancreatic cell development
negative regulation of glucocorticoid receptor signaling pathway
regulation of transcription from RNA polymerase II promoter
photoperiodism
entrainment of circadian clock
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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