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Invitrogen
CRBN Polyclonal Antibody
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 7
CRBN Antibody (PA5-98708) in ICC/IF
Immunofluorescence analysis of Protein cereblon was performed using HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CRBN Polyclonal Antibody (Product # PA5-98708) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000 d... View More
Product Details
PA5-98708
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Human Protein cereblon protein (1-280AA)
Conjugate
Form
Liquid
Concentration
0.59 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Wet ice
RRID
AB_2813321
Target Information
CRBN, a member of the Lon protease protein family, plays a role in brain development. It is a component of the DCX (DDB1-CUL4-X-box) E3 protein ligase complex, a complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins and is required for limb outgrowth and expression of the fibroblast growth factor FGF8. CRBN is thought to regulate the assembly and neuronal surface expression of large-conductance calcium-activated potassium channels in brain regions involved in memory and learning via its interaction with KCNT1. It is widely expressed and highly expressed in brain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp781K0715; MGC27358; novel lethal; Protein cereblon; Protein PiL; protein x 0001
Gene Aliases: 2610203G15Rik; 2900045O07Rik; AD-006; AF229032; AW108261; CRBN; MRT2; MRT2A; piL
UniProt ID: (Human) Q96SW2, (Mouse) Q8C7D2
Entrez Gene ID: (Human) 51185, (Mouse) 58799
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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