Invitrogen

CREB Monoclonal Antibody (LB9)

Advanced Verification

IP-mass spectrometry

3 Published Figures

8 References

View all (63) CREB antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

CREB Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 14

CREB Antibody (MA1-083) in ICC/IF

Immunofluorescence analysis of CREB was performed using 70% confluent log phase MDA-MB-231 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CREB Monoclonal Antibody (Product # MA1-083) at 5µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a di... View More

CREB Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CREB Antibody in Immunoprecipitation (IP)

Product Image Gallery

CREB Antibody — IP-MS enrichment of CREB1 (LFQ intensity): CREB1 was enriched 113-fold from A549 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Product # 90409) and CREB1 antibody (Product # MA1-083). The STRING database (www.string-db.org) was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity. {IP-MS}

Product Details

MA1-083

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:200

Immunocytochemistry (ICC/IF)

5 µg/mL

View 1 publication 1 publication

ELISA (ELISA)

0.1-1.0 µg/mL

Immunoprecipitation (IP)

2 µg

ChIP assay (ChIP)

2.5 µg/1x10^6 cells

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

LB9

Immunogen

Recombinant human CREB-A protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_558523

Product Specific Information

MA1-083 detects the CREB in human, monkey, rat and mouse cells.

MA1-083 has been successfully used in immunofluorescence, Western blot, immunoprecipitation, immunohistochemistry, and ELISA procedures. By Western blot, this antibody detects a ~43 kDa protein.

The MA1-083 immunogen is human recombinant CREB-A protein.

Target Information

CREB (Cyclic AMP response element binding protein) is a 43 kDa basic/leucine zipper transcription factor that binds the cyclic AMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth and neurotrophic factors. Activation of CREB is dependent upon the phosphorylation of serine 133. Phosphorylation of CREB occurs via p44/42 MAP kinase and p90RSK, and via p38 MAP kinase and MSK1. Although CREB will bind DNA independent of its phosphorylation state, only the phosphorylated form is competent as a transcription factor. CREB binding protein (CBP), a transcriptional coactivator that directly interacts with CREB, binds to CREB in the region of serine 133. CREB is involved in different cellular processes including the synchronization of circadian rhythmicity, differentiation of adipose cells, and learning and memory. In humans, the gene is located on the q arm of chromosome 2. Diseases associated with CREB dysfunction include Alzheimer’s Disease, histiocytoma and soft tissue melanoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Binding of YY1/CREB to an Enhancer Region Triggers Claudin 6 Expression in H. pylori LPS-Stimulated AGS Cells.

International journal of molecular sciences

Romero-Estrada JH,Montaño LF,Rendón-Huerta EP

Published figure using CREB monoclonal antibody (Product # MA1-083) in ChIP Assay

Tue Sep 12 00:00:00 EDT 2023

Jacob-induced transcriptional inactivation of CREB promotes Aβ-induced synapse loss in Alzheimer's disease.

The EMBO journal

Grochowska KM,Gomes GM,Raman R,Kaushik R,Sosulina L,Kaneko H,Oelschlegel AM,Yuanxiang P,Reyes-Resina I,Bayraktar G,Samer S,Spilker C,Woo MS,Morawski M,Goldschmidt J,Friese MA,Rossner S,Navarro G,Remy S,Reissner C,Karpova A,Kreutz MR

MA1-083 was used in Western Blotting to investigate β-amyloid peptide in synapses in Alzheimer's disease.

Wed Feb 15 00:00:00 EST 2023

Studying Circadian Clock Entrainment by Hormonal Signals.

Methods in molecular biology (Clifton, N.J.)

Pilorz V,Olejniczak I,Oster H

MA1-083 was used in ChIP Assay, ELISA, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot to propose an experimental procedure based on an in vitro setup consisting of several consecutive steps in which organotypic tissue cultures or cells can be used.

Thu May 26 00:00:00 EDT 2022

Lamin B1 Accumulation's Effects on Autosomal Dominant Leukodystrophy (ADLD): Induction of Reactivity in the Astrocytes.

Cells

Ratti S,Rusciano I,Mongiorgi S,Neri I,Cappellini A,Cortelli P,Suh PG,McCubrey JA,Manzoli L,Cocco L,Ramazzotti G

Published figure using CREB monoclonal antibody (Product # MA1-083) in Western Blot

Tue Sep 28 00:00:00 EDT 2021

The transcription factor CREB acts as an important regulator mediating oxidative stress-induced apoptosis by suppressing αB-crystallin expression.

Aging

Wang L,Nie Q,Gao M,Yang L,Xiang JW,Xiao Y,Liu FY,Gong XD,Fu JL,Wang Y,Nguyen QD,Liu Y,Liu M,Li DW

MA1-083 was used in Immunocytochemistry to demonstrate that CREB is an important transcription factor mediating stress response, and it promotes oxidative stress-induced apoptosis by suppressing αB-crystallin expression.

Wed Jun 17 00:00:00 EDT 2020

S-nitrosylation of E3 ubiquitin-protein ligase RNF213 alters non-canonical Wnt/Ca+2 signaling in the P301S mouse model of tauopathy.

Translational psychiatry

Amal H,Gong G,Gjoneska E,Lewis SM,Wishnok JS,Tsai LH,Tannenbaum SR

MA1-083 was used in Western Blotting to examine changes in the S-nitrosoproteome in 2-month-old transgenic P301S mice in order to detect molecular events corresponding to early stages of tauopathy progression.

Tue Jan 29 00:00:00 EST 2019

Chenodeoxycholic Acid Ameliorates AlCl₃-Induced Alzheimer's Disease Neurotoxicity and Cognitive Deterioration via Enhanced Insulin Signaling in Rats.

Molecules (Basel, Switzerland)

Bazzari FH,Abdallah DM,El-Abhar HS

MA1-083 was used in Western Blotting to show CDCA was able to improve insulin sensitivity in the hippocampi of AlCl3-treated rats, which highlights its potential in AD management.

Fri May 24 00:00:00 EDT 2019

Mitochondrial remodeling in mice with cardiomyocyte-specific lipid overload.

Journal of molecular and cellular cardiology

Elezaby A,Sverdlov AL,Tu VH,Soni K,Luptak I,Qin F,Liesa M,Shirihai OS,Rimer J,Schaffer JE,Colucci WS,Miller EJ

Published figure using CREB monoclonal antibody (Product # MA1-083) in Western Blot

Sun Feb 01 00:00:00 EST 2015

Bioinformatics

Protein Aliases: active transcription factor CREB; cAMP response element binding protein 1; cAMP-response element-binding protein-1; cAMP-responsive element-binding protein 1; CREB-1; cyclic adenosine 3',5'-monophosphate response element-binding protein CREB; Cyclic AMP-responsive element-binding protein 1; htCREB; MGC9284; OTTHUMP00000206661; OTTHUMP00000206662; OTTHUMP00000206667; transactivator protein; Y protein

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Gene Aliases: 2310001E10Rik; 3526402H21Rik; AV083133; CREB; CREB-1; CREB1

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UniProt ID: (Human) P16220, (Mouse) Q01147, (Rat) P15337

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Entrez Gene ID: (Human) 1385, (Mouse) 12912, (Rat) 81646

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II activating transcription factor binding transcriptional activator activity, RNA polymerase II transcription factor binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding transcription cofactor activity protein binding enzyme binding Hsp70 protein binding histone acetyltransferase binding cAMP response element binding identical protein binding arrestin family protein binding DNA binding double-stranded DNA binding transcription factor binding sequence-specific DNA binding transcription regulatory region DNA binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CREB Monoclonal Antibody (LB9)

CREB Antibody (MA1-083) in ICC/IF

Product Details

MA1-083

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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