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CRY1 Antibody (PA1-527) in ICC/IF
Immunofluorescence analysis of Cryptochrome 1 was performed using 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Cryptochrome 1 Rabbit Polyclonal Antibody (Product # PA1-527) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 con... View More
Product Details
PA1-527
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic Peptide: C Q(594) S V G P K V Q R Q S S N(606)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2276576
Product Specific Information
PA1-527 detects recombinant human cryptochrome 1 (Cry1).
PA1-527 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~70 kDa protein representing full length recombinant human Cry1 protein.
PA1-527 immunizing peptide corresponds to amino acid residues 594-606 from mouse Cry1. PA1-527 immunizing peptide (Cat. # PEP-103) is available for use in neutralization and control procedures.
Target Information
Various biochemical, physiological and behavioral processes display circadian rhythms controlled by an internal biological clock. The central "gears" driving this clock appear to be composed of an autoregulatory transcription/post translation-based feedback loop. Cryptochrome 1 (CRY1) and 2 (CRY2) are DNA-binding flavoproteins that bear some homology to blue-light receptors and photolyases. In Drosophila, CRY is a photoreceptor for the circadian clock where it binds to the clock component TIM in a light-dependent fashion and blocks its function. Mammalian CRY1 and CRY2 function via light-independent interactions with circadian genes CLOCK and BMAL1, as well as with PER1, PER2, and TIM. They seem to act as light-independent components of the circadian clock and likely regulate Per1 transcriptional cycling via interactions with both the activator and its feedback inhibitors. Mutant mice not expressing the Cry1 or Cry2 protein display accelerated and delayed periodicity of locomotor activity, respectively. It appears that the combination of both proteins working together is essential to synchronize the organism to circadian phases. A critical balance between Cry1 and Cry2 is required for proper clock function; in complete darkness, double-mutant mice present with instantaneous arrhythmicity, indicating the absence of an internal circadian clock.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: cryptochrome 1 (photolyase-like); Cryptochrome-1
Gene Aliases: AU020726; AU021000; CRY1; PHLL1
UniProt ID: (Human) Q16526, (Mouse) P97784
Entrez Gene ID: (Human) 1407, (Mouse) 12952
nucleotide binding
transcription factor activity, transcription factor binding
core promoter sequence-specific DNA binding
core promoter binding
DNA binding
double-stranded DNA binding
deoxyribodipyrimidine photo-lyase activity
DNA (6-4) photolyase activity
protein binding
transcription factor binding
blue light photoreceptor activity
protein kinase binding
phosphatase binding
nuclear hormone receptor binding
histone deacetylase binding
ubiquitin binding
DNA photolyase activity
photoreceptor activity
kinase binding
DNA photolyase
negative regulation of transcription from RNA polymerase II promoter
gluconeogenesis
transcription, DNA-templated
DNA damage induced protein phosphorylation
blue light signaling pathway
protein-chromophore linkage
lipid storage
negative regulation of protein ubiquitination
response to insulin
circadian regulation of gene expression
response to glucagon
glucose homeostasis
regulation of circadian rhythm
negative regulation of circadian rhythm
entrainment of circadian clock by photoperiod
negative regulation of G-protein coupled receptor protein signaling pathway
negative regulation of transcription, DNA-templated
regulation of DNA damage checkpoint
negative regulation of glucocorticoid receptor signaling pathway
negative regulation of glucocorticoid secretion
DNA repair
regulation of transcription, DNA-templated
circadian rhythm
rhythmic process
response to stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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