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CRYAB Antibody (MA5-45201) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with Alpha B Crystallin monoclonal antibody (Product # MA5-45201) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000, 1:5,000 for 60 min RT, 5 min RT. Localization: Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-A... View More
Product Details
MA5-45201
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Pig,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
1A7.D5
Immunogen
Native Alpha B Crystallin
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
47.35mM phosphate/2.15mM MES, pH 7.2, with 2mM EDTA, 0.17066µM Pentachlorophenol, 0.8mM ethanol
Contains
no preservative
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2931655
Product Specific Information
0.5 µg/mL of MA5-45201 was sufficient for detection of 50 ng purified alpha B crystalline by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 20kDa (Predicted mol. weight is approximately 21kDa). Does not cross-react with alpha A-crystallin, beta L-crystallin, beta H-crystallin, gamma -crystallin, HSP25, HSP27 or HSP47 proteins.
Target Information
Lens proteins consist almost entirely of crystallins (about 95%). Crystallins are also found vertebrate skeletal muscle tissue. In the lens, their structural function is to assist in maintaining the proper refractive index of the lens. The mammalian lens contains 3 major classes of crystallins: alpha, beta, and gamma. Alpha-crystallin is the largest of the crystallins and is composed of 2 primary gene products-alpha-A and alpha-B. There are at least 5 different proteins comprising the beta-crystallins. The gamma-crystallins are monomeric, but there are at least 5 gamma crystallins identified in bovine and rat lens. Alpha-Crystallin comprises 40% of total lens protein composition. In addition to maintaining proper refractive index, it also functions in a chaperone like manner by preventing the formation of aggregates possibly leading to cataract formation. It is believed that the phosphorylated states of the alpha-crystallin occur in response to cellular stress and may serve a structural control function and play a role in protein maintenance. Alpha-B crystallin has been linked to Alexander's disease where it accumulates in brain cells of those afflicted.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: alpha crystallin B; Alpha(B)-crystallin; Alpha-crystallin B chain; CryAB antibody; crystallin, alpha B; crystallin, alpha polypeptide 2; epididymis secretory protein Li 101; Heat shock protein beta-5; Heat shock protein family B member 5; heat-shock 20 kD like-protein; HspB5; Renal carcinoma antigen NY-REN-27; Rosenthal fiber component
Gene Aliases: AACRYA; CMD1II; CRYA2; CRYAB; CTPP2; CTRCT16; HEL-S-101; HSPB5; MFM2
UniProt ID: (Human) P02511, (Bovine) P02510, (Pig) Q7M2W6, (Rat) P23928
Entrez Gene ID: (Human) 1410, (Bovine) 281719, (Pig) 100519789, (Rat) 25420
response to hypoxia
lens development in camera-type eye
protein folding
muscle contraction
tubulin complex assembly
muscle organ development
aging
negative regulation of gene expression
regulation of cell death
negative regulation of cell growth
microtubule polymerization or depolymerization
response to estradiol
negative regulation of intracellular transport
response to hydrogen peroxide
negative regulation of apoptotic process
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
protein homooligomerization
stress-activated MAPK cascade
apoptotic process involved in morphogenesis
cellular response to gamma radiation
regulation of cellular response to heat
negative regulation of reactive oxygen species metabolic process
negative regulation of transcription, DNA-templated
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