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Invitrogen
CTCF Recombinant Rabbit Monoclonal Antibody (ARC0067)
Advanced Verification
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.
Product Details
MA5-35311
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
ARC0067
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 628-727 of human CTCF (P49711)
Conjugate
Form
Liquid
Concentration
0.4 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2849213
Product Specific Information
Immunogen sequence: PAVEIEPEPE PQPVTPAPPP AKKRRGRPPG RTNQPKQNQP TAIIQVEDQN TGAIENIIVE VKKEPDAEPA EGEEEEAQPA ATDAPNGDLT PEMILSMMDR
Target Information
Transcriptional factor CTCF is a ubiquitously expressed 11-zinc finger domain containing, DNA-binding nuclear phosphoprotein that is involved in multiple aspects of normal gene regulation including transcriptional repression and activation, gene silencing, chromatin insulation and regulation of imprinted sites. CTCF uses different combinations of ZF domains and interacts with the CCCTC motif on the DNA. It can bind to HAT- and HDAC-containing complexes thereby influencing transcriptional activation or repression respectively. It is a multifunctional protein involved in the transactivation of APPB gene, silencing of c-myc gene, and insulation of human beta-globin and DMI myotonic dystrophy locus and imprinting control of IGF2 and H19 regions. CTCF has also been shown to regulate the transcription of Pax6 and IRAK2 promoter thereby opening a therapeutic avenue. Mutations in this gene have been associated with invasive breast cancers, prostate cancers and Wilms' tumors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 11 zinc finger transcriptional repressor; 11-zinc finger protein; CCCTC-binding factor; CCCTC-binding factor (zinc finger protein); CTCFL paralog; OTTHUMP00000197610; Transcriptional repressor CTCF
Gene Aliases: AW108038; CTCF; MRD21
UniProt ID: (Human) P49711, (Rat) Q9R1D1, (Mouse) Q61164
Entrez Gene ID: (Human) 10664, (Rat) 83726, (Mouse) 13018
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
transcription corepressor activity
protein binding
zinc ion binding
chromatin insulator sequence binding
sequence-specific DNA binding
transcription regulatory region DNA binding
DNA binding
chromatin binding
metal ion binding
nucleic acid binding
C2H2 zinc finger transcription factor
negative regulation of transcription from RNA polymerase II promoter
DNA methylation
regulation of gene expression by genetic imprinting
transcription from RNA polymerase II promoter
chromosome segregation
maintenance of DNA methylation
positive regulation of gene expression
covalent chromatin modification
nucleosome positioning
regulation of histone methylation
regulation of histone acetylation
regulation of gene expression, epigenetic
regulation of molecular function, epigenetic
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
regulation of centromeric sister chromatid cohesion
transcription, DNA-templated
regulation of transcription, DNA-templated
negative regulation of gene expression
chromatin modification
dosage compensation by inactivation of X chromosome
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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