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CXCL1 Antibody (500-P115BT-25UG) in WB
Western Blot: To detect mKC by Western Blot analysis CXCL1 Polyclonal Antibody, Biotin (Product # 500-P115BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant mKC is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P115BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Murine KC (CXCL1)
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929286
Product Specific Information
AA Sequence of recombinant protein: APIANELRCQ CLQTMAGIHL KNIQSLKVLP SGPHCTQTEV IATLKNGREA CLDPEAPLVQ KIVQKMLKGV PK.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine KC (CXCL1). Anti-Murine KC (CXCL1)-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Murine KC (CXCL1) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine KC (CXCL1) (500-P115) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine KC (CXCL1).
Western Blot: To detect mKC by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant mKC is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P115BT-1MG will be provided as 2 x 500 µg
Target Information
CXCL1, CXCL2, and CXCL3, also known respectively as GRO alpha, GRO beta (MIP-2 alpha) and GRO gamma (MIP-2 beta), are members of the CXC subfamily of chemokines. Mature CXCL1/2/3 proteins bind with high affinity to the IL-8 receptor type B and are potent neutrophil attractants and activators. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: alpha-chemokine; C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 1; CC motif chemokine; CCmotif chemokine; CXC; CXC motif chemokine; CXCL; CXCL-1; GRO a; GRO α; GRO1 oncogene; GROalpha; Growth-regulated alpha protein; GROα; KC/GR)-alpha; KC/GRO-alpha; NAP3; Platelet-derived growth factor-inducible protein KC; Secretory protein N51
Gene Aliases: Cxcl1; Fsp; Gro; Gro1; KC; Mgsa; N51; Scyb1
UniProt ID: (Mouse) P12850
Entrez Gene ID: (Mouse) 14825
response to molecule of bacterial origin
acute inflammatory response
positive regulation of leukocyte chemotaxis
inflammatory response
immune response
G-protein coupled receptor signaling pathway
positive regulation of cytosolic calcium ion concentration
positive regulation of sodium ion transport
neutrophil chemotaxis
response to lipopolysaccharide
positive regulation of superoxide anion generation
positive regulation of potassium ion transport
cell chemotaxis
chemokine-mediated signaling pathway
positive regulation of neutrophil mediated killing of fungus
positive regulation of hematopoietic stem cell proliferation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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