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Invitrogen
CXCL1 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Neutralization to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 4
CXCL1 Antibody (PA5-46960) in IHC (P)
Immunohistochemical analysis of CXCL1 in perfusion fixed paraffin-embedded sections of rat liver. Samples were incubated in CXCL1 polyclonal antibody (Product # PA5-46960) using a dilution of 15 µg/mL overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue).
Product Details
PA5-46960
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rat
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived recombinant rat CXCL1/GRO alpha/KC/CINC-1 Ala25-Lys96
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2609427
Product Specific Information
In direct ELISAs, less than 50% cross-reactivity with recombinant mouse (rm) KC is observed and less than 10% cross-reactivity with recombinant human (rh) GRO alpha, rhGRO beta, rhGRO gamma, rmMIP-2, recombinant rat (rr) CINC-2 alpha, rrCINC-2 beta, and rrCINC-3.
Reconstitute at 0.2 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
Target Information
CXCL1, CXCL2, and CXCL3, also known respectively as GRO alpha, GRO beta (MIP-2 alpha) and GRO gamma (MIP-2 beta), are members of the CXC subfamily of chemokines. Mature CXCL1/2/3 proteins bind with high affinity to the IL-8 receptor type B and are potent neutrophil attractants and activators. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 1; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha); CINC-1; CXC; CXC motif chemokine; CXCL; CXCL-1; Cytokine-induced neutrophil chemoattractant 1; gro; GRO a; GRO α; GROalpha; Growth-regulated alpha protein; GROα; NAP3; Platelet-derived growth factor-inducible protein KC
Gene Aliases: CINC-1; Cinc1; Cxcl1; Gro; Gro1; Scyb1
UniProt ID: (Rat) P14095
Entrez Gene ID: (Rat) 81503
response to amphetamine
response to molecule of bacterial origin
acute inflammatory response
positive regulation of leukocyte chemotaxis
immune response
G-protein coupled receptor signaling pathway
positive regulation of cytosolic calcium ion concentration
response to heat
response to gamma radiation
positive regulation of sodium ion transport
neutrophil chemotaxis
response to estradiol
response to lipopolysaccharide
positive regulation of superoxide anion generation
regulation of cell proliferation
positive regulation of potassium ion transport
response to glucocorticoid
cell chemotaxis
chemokine-mediated signaling pathway
positive regulation of neutrophil mediated killing of fungus
positive regulation of hematopoietic stem cell proliferation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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