Invitrogen

CXCL1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

4 Published Figures

7 References

View all (31) CXCL1 (GRO alpha) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

CXCL1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 11

CXCL1 Antibody (PA5-86508) in ICC/IF

Immunofluorescence analysis of Growth-regulated alpha protein was performed using 70% confluent log phase A-375 cells treated with 10 ng/mL TNF alpha for 24 hours and untreated A-375 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CXCL1 Polyclonal Antibody (Product # PA5-86508) at 1:100 dilution in 0.1% BSA, incubated at 4°C overnight and then labeled with Donkey anti-Rabbit IgG (H+L... View More

CXCL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CXCL1 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

CXCL1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. Relative expression of Growth-regulated alpha protein was observed in Caki-1 (positive model) and HT-29 (negative model) using Anti-CXCL1 Polyclonal Antibody (Product # PA5-86508) in Western Blot. {RE}

Product Details

PA5-86508

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide, corresponding to amino acids 41-90 of Human GROalpha.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2803283

Product Specific Information

Purity is > 95% by SDS-PAGE.

Target Information

CXCL1, CXCL2, and CXCL3, also known respectively as GRO alpha, GRO beta (MIP-2 alpha) and GRO gamma (MIP-2 beta), are members of the CXC subfamily of chemokines. Mature CXCL1/2/3 proteins bind with high affinity to the IL-8 receptor type B and are potent neutrophil attractants and activators. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

E3 ligase TRIM28 promotes anti-PD-1 resistance in non-small cell lung cancer by enhancing the recruitment of myeloid-derived suppressor cells.

Journal of experimental & clinical cancer research : CR

Liang M,Sun Z,Chen X,Wang L,Wang H,Qin L,Zhao W,Geng B

Published figure using CXCL1 polyclonal antibody (Product # PA5-86508) in Immunohistochemistry

Sat Oct 21 00:00:00 EDT 2023

Pharmacological perturbation of CXCL1 signaling alleviates neuropathogenesis in a model of HEVA71 infection.

Nature communications

Gunaseelan S,Ariffin MZ,Khanna S,Ooi MH,Perera D,Chu JJH,Chua JJE

PA5-86508 was used in Immunohistochemistry to highlight the CXCL1-CXCR2 signaling pathway as a potential target against HFMD neuropathogenesis.

Wed Feb 16 00:00:00 EST 2022

Associations of C-X-C motif chemokine ligands 1/2/8/13/14 with clinicopathological features and survival profile in patients with colorectal cancer.

Oncology letters

Luo X,Tai J,Zhao Y,Zhao P,Sun D,Wang L

PA5-86508 was used in Immunohistochemistry to assess the correlation of C-X-C motif chemokine ligand (CXCL)1, CXCL2, CXCL8, CXCL13 and CXCL14 with clinicopathological features and survival profile in patients with colorectal cancer (CRC).

Sat Oct 01 00:00:00 EDT 2022

LncRNA MEG3 ameliorates NiO nanoparticles-induced pulmonary inflammatory damage via suppressing the p38 mitogen activated protein kinases pathway.

Environmental toxicology

Yang M,Chang X,Gao Q,Gong X,Zheng J,Liu H,Li K,Zhan H,Wang X,Li S,Sun X,Feng S,Sun Y

PA5-86508 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to explore the roles of long noncoding RNA maternally expressed gene 3 (lncRNA MEG3) and p38 mitogen activated protein kinases (p38 MAPK) pathway in pulmonary inflammatory injury induced by NiO NPs.

Sun May 01 00:00:00 EDT 2022

Monovalent antibody-conjugated lipid-polymer nanohybrids for active targeting to desmoglein 3 of keratinocytes to attenuate psoriasiform inflammation.

Theranostics

Lin ZC,Hwang TL,Huang TH,Tahara K,Trousil J,Fang JY

PA5-86508 was used in Immunohistochemistry to develop actively targeted nanocarriers loaded with the phosphodiesterase 4 inhibitor AN2728.

Mon Aug 16 00:00:00 EDT 2021

Monovalent antibody-conjugated lipid-polymer nanohybrids for active targeting to desmoglein 3 of keratinocytes to attenuate psoriasiform inflammation.

Theranostics

Lin ZC,Hwang TL,Huang TH,Tahara K,Trousil J,Fang JY

PA5-86508 was used in Immunohistochemistry to develop actively targeted nanocarriers loaded with the phosphodiesterase 4 inhibitor AN2728.

Mon Aug 16 00:00:00 EDT 2021

CXCL1 Regulated by miR-302e Is Involved in Cell Viability and Motility of Colorectal Cancer via Inhibiting JAK-STAT Signaling Pathway.

Frontiers in oncology

Chen B,Song L,Nie X,Lin F,Yu Z,Kong W,Qi X,Wang W

PA5-86508 was used in Immunohistochemistry (Paraffin) to make a systemic description for the CXCL1-dependent regulatory mechanism in colorectal cancer.

Sat Apr 23 00:00:00 EDT 2022

CXCL1 Regulated by miR-302e Is Involved in Cell Viability and Motility of Colorectal Cancer via Inhibiting JAK-STAT Signaling Pathway.

Frontiers in oncology

Chen B,Song L,Nie X,Lin F,Yu Z,Kong W,Qi X,Wang W

PA5-86508 was used in Immunohistochemistry (Paraffin) to make a systemic description for the CXCL1-dependent regulatory mechanism in colorectal cancer.

Sat Apr 23 00:00:00 EDT 2022

Involvement of CXCL1/CXCR2 During Microglia Activation Following Inflammation-Sensitized Hypoxic-Ischemic Brain Injury in Neonatal Rats.

Frontiers in neurology

Serdar M,Kempe K,Herrmann R,Picard D,Remke M,Herz J,Bendix I,Felderhoff-Müser U,Sabir H

PA5-86508 was used in Western Blot to indicate that the CXCL1/CXCR2 pathway may be involved during pro-inflammatory microglia activation following inflammation-sensitized hypoxic-ischemic brain injury in neonatal rats.

Sat Oct 31 00:00:00 EDT 2020

Involvement of CXCL1/CXCR2 During Microglia Activation Following Inflammation-Sensitized Hypoxic-Ischemic Brain Injury in Neonatal Rats.

Frontiers in neurology

Serdar M,Kempe K,Herrmann R,Picard D,Remke M,Herz J,Bendix I,Felderhoff-Müser U,Sabir H

Sat Oct 31 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: alpha-chemokine; C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 1; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha); CINC-1; CXC; CXC motif chemokine; CXCL; CXCL-1; Cytokine-induced neutrophil chemoattractant 1; fibroblast secretory protein; gro; GRO a; GRO α; GRO-alpha(1-73); GRO1 oncogene; GRO1 oncogene (melanoma growth stimulating activity, alpha); GRO1 oncogene (melanoma growth-stimulating activity); GROalpha; Growth-regulated alpha protein; GROα; KC/GR)-alpha; KC/GRO-alpha; melanoma growth stimulating activity, alpha; Melanoma growth stimulatory activity; melanoma growth stimulatory activity alpha; MGSA; MGSA alpha; NAP-3; NAP3; Neutrophil-activating protein 3; Platelet-derived growth factor-inducible protein KC; Secretory protein N51

View more View less

Gene Aliases: CINC-1; Cinc1; CXCL1; FSP; GRO; GRO1; GROA; KC; MGSA; MGSA-a; N51; NAP-3; SCYB1

View more View less

UniProt ID: (Human) P09341, (Rat) P14095, (Mouse) P12850

View more View less

Entrez Gene ID: (Human) 2919, (Rat) 81503, (Mouse) 14825

View more View less

Function(s)

receptor binding chemokine activity enzyme activator activity growth factor activity CXCR chemokine receptor binding cytokine activity chemokine

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CXCL1 Polyclonal Antibody

CXCL1 Antibody (PA5-86508) in ICC/IF

Product Details

PA5-86508

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!