Invitrogen

CYP8B1 Polyclonal Antibody

2 Published Figures

6 References

View all (4) CYP8B1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (2)
Published Figures (2)

FIGURE: 1 / 4

CYP8B1 Antibody (PA5-37088) in WB

Western blot analysis of CYP8B1 in Lane 1: HEK293T cell lysate, Lane 2: sp2/0 cell lysate, Lane 3: PC12 cell lysate. Samples were incubated with CYP8B1 polyclonal antibody (Product # PA5-37088) at a dilution of 1:500.

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Product Details

PA5-37088

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to amino acids 416-461 of human CYP8B1

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2553905

Product Specific Information

This antibody detects endogenous protein at a molecular weight of 58 kDa.

Purity is >95% by SDS-PAGE.

Target Information

CYP8B1 (sterol 12-alpha-hydroxylase) is a member of the cytochrome P450 superfamily of monooxygenase enzymes that are involved in the metabolism of a wide array of endogenous and xenobiotic compounds. CYP8B1 is highly expressed in liver and is an important enzyme for bile acid synthesis. Specifically, CYP8B1 moderates the ratio of cholic acid over chenodeoxycholic acid to control the solubility of cholesterol. The gene encoding human CYP8B1 maps to chromosome 3p22.1. The CYP8B1 gene encodes a 501-amino acid protein and does not contain any introns. The CYP8B1 gene promoter is transactivated by hepatocyte nuclear factor 4a. In mice, disruption of the CYP8B1 gene prevents the synthesis of cholate, which is a primary bile acid.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Akkermansia muciniphila protects mice against an emerging tick-borne viral pathogen.

Nature microbiology

Xie J,Li H,Zhang X,Yang T,Yue M,Zhang Y,Chen S,Cui N,Yuan C,Li J,Zhu SJ,Liu W

PA5-37088 was used in Immunohistochemistry (Paraffin), Western Blot to indicate the probiotic potential of A. muciniphila in mitigating SFTSV infection.

Sun Jan 01 00:00:00 EST 2023

The metabolic genomic atlas reveals potential drivers and clinically relevant insights into the etiology of esophageal squamous cell carcinoma.

Theranostics

Liu X,Hong R,Du P,Yang D,He M,Wu Q,Li L,Wang Y,Chen J,Min Q,Li J,Zhang W,Zhan Q

PA5-37088 was used in Immunohistochemistry and Western Blotting to provide a deep insight into the metabolic landscape in Esophageal squamous cell carcinoma (ESCC), extending our understanding of the metabolic reconfiguration underlying the genomic basis of ESCC.

Thu Sep 29 00:00:00 EDT 2022

The metabolic genomic atlas reveals potential drivers and clinically relevant insights into the etiology of esophageal squamous cell carcinoma.

Theranostics

Liu X,Hong R,Du P,Yang D,He M,Wu Q,Li L,Wang Y,Chen J,Min Q,Li J,Zhang W,Zhan Q

Thu Sep 29 00:00:00 EDT 2022

Multidrug Resistance-Associated Protein 2 Deficiency Aggravates Estrogen-Induced Impairment of Bile Acid Metabolomics in Rats.

Frontiers in physiology

Alaei Faradonbeh F,Lastuvkova H,Cermanova J,Hroch M,Nova Z,Uher M,Hirsova P,Pavek P,Micuda S

PA5-37088 was used in Immunohistochemistry (Paraffin), Western Blot to demonstrate that Mrp2-deficient organism is more sensitive to estrogen-induced cholestasis.

Sat Jul 16 00:00:00 EDT 2022

Hepatocyte activity of the cholesterol sensor smoothened regulates cholesterol and bile acid homeostasis in mice.

iScience

Dalton GD,Oh SH,Tang L,Zhang S,Brown AL,Varadharajan V,Baleanu-Gogonea C,Gogonea V,Pathak P,Brown JM,Diehl AM

PA5-37088 was used in Immunohistochemistry (Paraffin), Western Blot to demonstrate G-protein-coupled receptor Smoothened in hepatocytes plays a regulatory role in sensing and feedback regulation of cholesterol balance driven by excess dietary cholesterol.

Fri Sep 24 00:00:00 EDT 2021

Glucocorticoids regulate AKR1D1 activity in human liver in vitro and in vivo.

The Journal of endocrinology

Nikolaou N,Arvaniti A,Appanna N,Sharp A,Hughes BA,Digweed D,Whitaker MJ,Ross R,Arlt W,Penning TM,Morris K,George S,Keevil BG,Hodson L,Gathercole LL,Tomlinson JW

PA5-37088 was used in Western Blotting to examine the impact of glucocorticoids on Steroid 5β-reductase (AKR1D1) activity in human liver HepG2 and Huh7 cells.

Fri May 01 00:00:00 EDT 2020

Glucocorticoids regulate AKR1D1 activity in human liver in vitro and in vivo.

The Journal of endocrinology

Nikolaou N,Arvaniti A,Appanna N,Sharp A,Hughes BA,Digweed D,Whitaker MJ,Ross R,Arlt W,Penning TM,Morris K,George S,Keevil BG,Hodson L,Gathercole LL,Tomlinson JW

PA5-37088 was used in Western Blotting to examine the impact of glucocorticoids on Steroid 5β-reductase (AKR1D1) activity in human liver HepG2 and Huh7 cells.

Fri May 01 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: 7 alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase; 7-alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase; 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase; CYPVIIIB1; Cytochrome P450 8B1; cytochrome P450 8b1 sterol 12 alpha-hydrolase; cytochrome P450, 8b1, sterol 12 alpha-hydrolase; cytochrome P450, family 8, subfamily B, polypeptide 1; cytochrome P450, subfamily VIIIB (sterol 12-alpha-hydroxylase), polypeptide 1; sterol 12-alpha-hydrolase; Sterol 12-alpha-hydroxylase

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Gene Aliases: CP8B; CYP12; CYP8B1

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UniProt ID: (Human) Q9UNU6, (Mouse) O88962

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Entrez Gene ID: (Human) 1582, (Mouse) 13124, (Rat) 81924

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Function(s)

iron ion binding sterol 12-alpha-hydroxylase activity oxygen binding heme binding 7alpha-hydroxycholest-4-en-3-one 12alpha-hydroxylase activity monooxygenase activity oxidoreductase activity oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen metal ion binding

Process(es)

lipid metabolic process oxidation-reduction process bile acid biosynthetic process sterol metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CYP8B1 Polyclonal Antibody

CYP8B1 Antibody (PA5-37088) in WB

Product Details

PA5-37088

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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