Invitrogen

Carbonic Anhydrase IX Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

8 Published Figures

8 References

View all (64) Carbonic Anhydrase IX antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Carbonic Anhydrase IX Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 16

Carbonic Anhydrase IX Antibody (PA1-16592) in IHC (P)

Immunohistochemical analysis of Carbonic Anhydrase IX in formalin-fixed paraffin-embedded tissue section of human breast cancer. Samples were incubated in Carbonic Anhydrase IX polyclonal antibody (Product # PA1-16592) using a dilution of 1:1000 followed by HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin. This CAIX antibody generated an expected cytop... View More

Carbonic Anhydrase IX Antibody in Western Blot (WB)

Carbonic Anhydrase IX Antibody in Immunocytochemistry (ICC/IF)

Carbonic Anhydrase IX Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

PA1-16592

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:500

Immunohistochemistry (Frozen) (IHC (F))

1:200-1:500

Immunocytochemistry (ICC/IF)

2-5 µg/mL

View 2 publications 2 publications

Flow Cytometry (Flow)

1:1,000

ELISA (ELISA)

Assay-Dependent

ChIP assay (ChIP)

1:10-1:500

in situ PLA (PLA)

Assay-Dependent

Gel Shift (GS)

Assay-Dependent

Product Specifications

Species Reactivity

Plant, Rat, Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide derived from a C-terminal sequence of the human CA IX.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_568476

Product Specific Information

Suggested positive control: antigen standard for CA9 (transient overexpression lysate), Renal carcinoma.

Target Information

Carbonic anhydrase (CA) is an enzyme that assists rapid interconversion of carbon dioxide and water into carbonic acid, protons, and bicarbonate ions. It is abundant in all mammalian tissues. There are many genes that are inducible by hypoxia, via HIF-1 alpha. CA IX is one of the most inducible genes because of its stability and location within the membrane. Carbonic anhydrases have a widespread role in regulating pH in normal tissues, by regulating hydrogen ion (H+) flux. The pH is important in cell death under hypoxia, thus a blockade of CA IX results in increased cell death under hypoxia. Therefore, CA IX has become a reliable histochemical marker of hypoxia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Hypoxia-mediated stabilization of HIF1A in prostatic intraepithelial neoplasia promotes cell plasticity and malignant progression.

Science advances

Abu El Maaty MA,Terzic J,Keime C,Rovito D,Lutzing R,Yanushko D,Parisotto M,Grelet E,Namer IJ,Lindner V,Laverny G,Metzger D

PA1-16592 was used in ChIP Assay, ELISA, Flow Cytometry, Gel Shift, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Proximity Ligation Assay, Western Blot to characterize prostatic intraepithelial neoplasia (PIN) progression and demonstrate that HIF1A is a target for PCa prevention and that TGM2 is a promising prognostic biomarker of early relapse after prostatectomy.

Fri Jul 22 00:00:00 EDT 2022

Hypoxia-mediated stabilization of HIF1A in prostatic intraepithelial neoplasia promotes cell plasticity and malignant progression.

Science advances

Abu El Maaty MA,Terzic J,Keime C,Rovito D,Lutzing R,Yanushko D,Parisotto M,Grelet E,Namer IJ,Lindner V,Laverny G,Metzger D

Fri Jul 22 00:00:00 EDT 2022

Therapeutic Effects of Inhibition of Sphingosine-1-Phosphate Signaling in HIF-2α Inhibitor-Resistant Clear Cell Renal Cell Carcinoma.

Cancers

Hoefflin R,Harlander S,Abhari BA,Peighambari A,Adlesic M,Seidel P,Zodel K,Haug S,Göcmen B,Li Y,Lahrmann B,Grabe N,Heide D,Boerries M,Köttgen A,Heikenwalder M,Frew IJ

Published figure using Carbonic Anhydrase IX polyclonal antibody (Product # PA1-16592) in Immunohistochemistry

Sat Sep 25 00:00:00 EDT 2021

Hypoxic Jumbo Spheroids On-A-Chip (HOnAChip): Insights into Treatment Efficacy.

Cancers

Refet-Mollof E,Najyb O,Chermat R,Glory A,Lafontaine J,Wong P,Gervais T

Published figure using Carbonic Anhydrase IX polyclonal antibody (Product # PA1-16592) in Immunocytochemistry

Wed Aug 11 00:00:00 EDT 2021

HIF-1α and HIF-2α differently regulate tumour development and inflammation of clear cell renal cell carcinoma in mice.

Nature communications

Hoefflin R,Harlander S,Schäfer S,Metzger P,Kuo F,Schönenberger D,Adlesic M,Peighambari A,Seidel P,Chen CY,Consenza-Contreras M,Jud A,Lahrmann B,Grabe N,Heide D,Uhl FM,Chan TA,Duyster J,Zeiser R,Schell C,Heikenwalder M,Schilling O,Hakimi AA,Boerries M,Frew IJ

PA1-16592 was used in Immunohistochemistry to show using an autochthonous ccRCC model that Hif1a is essential for tumour formation whereas Hif2a deletion has only minor effects on tumour initiation and growth.

Mon Aug 17 00:00:00 EDT 2020

Deletion of the von Hippel-Lindau Gene in Hemangioblasts Causes Hemangioblastoma-like Lesions in Murine Retina.

Cancer research

Wang H,Shepard MJ,Zhang C,Dong L,Walker D,Guedez L,Park S,Wang Y,Chen S,Pang Y,Zhang Q,Gao C,Wong WT,Wiley H,Pacak K,Chew EY,Zhuang Z,Chan CC

PA1-16592 was used in Western Blotting to provide a phenotypic recapitulation of von Hippel-Lindau (VHL)-associated retinal capillary hemangioblastomas (RCH) in a murine model that may be useful to study RCH pathogenesis and therapeutics aimed at treating ocular VHL.

Thu Mar 01 00:00:00 EST 2018

Mixing and delivery of multiple controlled oxygen environments to a single multiwell culture plate.

American journal of physiology. Cell physiology

Yao M,Sattler T,Rabbani ZN,Pulliam T,Walker G,Gamcsik MP

PA1-16592 was used in Immunohistochemistry-immunofluorescence to demonstrate a design which is capable of mixing and delivering differing oxygen environments to separate rows of a single multiwell culture plate.

Thu Nov 01 00:00:00 EDT 2018

Mixing and delivery of multiple controlled oxygen environments to a single multiwell culture plate.

American journal of physiology. Cell physiology

Yao M,Sattler T,Rabbani ZN,Pulliam T,Walker G,Gamcsik MP

Thu Nov 01 00:00:00 EDT 2018

Modeling Renal Cell Carcinoma in Mice: Bap1 and Pbrm1 Inactivation Drive Tumor Grade.

Cancer discovery

Gu YF,Cohn S,Christie A,McKenzie T,Wolff N,Do QN,Madhuranthakam AJ,Pedrosa I,Wang T,Dey A,Busslinger M,Xie XJ,Hammer RE,McKay RM,Kapur P,Brugarolas J

Published figure using Carbonic Anhydrase IX polyclonal antibody (Product # PA1-16592) in Immunohistochemistry

Tue Aug 01 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: CA-9; CA-IX; Carbonate dehydratase IX; Carbonic anhydrase 9; Carbonic anhydrase IX; carbonic dehydratase; Membrane antigen MN; Membrane antigen MN homolog; P54 / 58N; P54/58N; pMW1; RCC-associated antigen G250; RCC-associated protein G250; Renal cell carcinoma-associated antigen G250

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Gene Aliases: CA9; CAIX; Car9; G250; MN; MN/CA9

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UniProt ID: (Human) Q16790, (Mouse) Q8VHB5

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Entrez Gene ID: (Human) 768, (Mouse) 230099, (Rat) 313495

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Function(s)

carbonate dehydratase activity zinc ion binding lyase activity metal ion binding molecular_function

Process(es)

morphogenesis of an epithelium one-carbon metabolic process secretion response to hypoxia response to testosterone response to drug bicarbonate transport regulation of transcription from RNA polymerase II promoter in response to hypoxia

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Carbonic Anhydrase IX Polyclonal Antibody

Carbonic Anhydrase IX Antibody (PA1-16592) in IHC (P)

Product Details

PA1-16592

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

ChIP assay (ChIP)

in situ PLA (PLA)

Gel Shift (GS)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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